乳腺癌中心体调控蛋白SEC23B在肿瘤浸润转移中的作用及其机制研究

冷婕, 邱国春, 张菠, 蒲艳

Mechanism of breast cancer centrosome regulatory protein SEC23B on tumor invasion and metastasis

LENG Jie, QIU Guochun, ZHANG Bo, PU Yan

图3 SEC23B诱导乳腺癌细胞自噬

Fig. 3 SEC23B induces autophagy in breast cancer cells A: Effect of SEC23B overexpression plasmid on p62, LCA/B expression in MCF-7 cells detected by Western blot; B: Representative images and quantitative analysis of the effect of SEC23B overexpression on autophagic granules in MCF-7 cells detected by immunofluorescence staining; C: Effect of SEC23B knockdown on p62, LCA/B expression in MDA231-LM2 cells by Western blot; D: Representative images and quantitative analysis of the effect of SEC23B knockdown on autophagic granules in MDA231-LM2 detected by immunofluorescence staining; E: Effect of CQ on migration of SEC23B overexpressing MCF-7 cells was assessed by transwell assay. **: P<0.01, compared with control or sh-NC groups; ***: P<0.001, compared with control or sh-NC groups.