乳腺癌中心体调控蛋白SEC23B在肿瘤浸润转移中的作用及其机制研究

冷婕, 邱国春, 张菠, 蒲艳

Mechanism of breast cancer centrosome regulatory protein SEC23B on tumor invasion and metastasis

LENG Jie, QIU Guochun, ZHANG Bo, PU Yan

图4 ERK/mTOR信号转导通路参与SEC23B在乳腺癌细胞中诱导的自噬

Fig. 4 ERK/mTOR pathway is involved in SEC23B-induced autophagy in breast cancer cells A: Effect of SEC23B overexpression plasmid on ERK/mTOR pathway expression in MCF-7 cells detected by Western blot; B: Effect of SEC23B knockdown on ERK/mTOR pathway expression in MDA231-LM2 cells detected by Western blot; C: Effect of EGF on ERK/mTOR pathway expression in MCF-7 cells detected by Western blot, effects of EGF on ERK/mTOR pathway and p62, LCA/B expression in SEC23B overexpressing MCF-7 cells detected by Western blot; D: Representative images and quantitative analysis of the effects of EGF on autophagic granules in SEC23B overexpressing MCF-7 cells detected by immunofluorescence staining. **: P<0.01, compared with the control group; #: P<0.05, compared with the SEC23B group.