Lymph node metastatic models of VX2 tumor in New Zealand white rabbits and their biological characteristics

孙轶群; 童　彤; 毛　健

doi:10.19401/j.cnki.1007-3639.2016.10.006

您当前的位置：

首页 >

文章列表页 >

Lymph node metastatic models of VX2 tumor in New Zealand white rabbits and their biological characteristics

更新时间：2025-12-31

- Lymph node metastatic models of VX2 tumor in New Zealand white rabbits and their biological characteristics
- China Oncology Vol. 26, Issue 10, Pages: 840-847(2016)
- 作者机构：
  
  复旦大学附属肿瘤医院放射诊断科，复旦大学上海医学院肿瘤学系,上海,200032
- 作者简介：
- 基金信息：
- DOI：10.19401/j.cnki.1007-3639.2016.10.006
  CLC：
- Published Online：17 November 2016，
  
  Published：17 November 2016
- 稿件说明：
移动端阅览
孙轶群,童　彤,毛　健,等. VX2移植性兔直肠癌淋巴结转移动物模型的建立及其生物学特性[J]. 中国癌症杂志, 2016, 26(10): 840-847.

孙轶群, 童　彤, 毛　健. Lymph node metastatic models of VX2 tumor in New Zealand white rabbits and their biological characteristics[J]. China Oncology, 2016, 26(10): 840-847.
孙轶群,童　彤,毛　健,等. VX2移植性兔直肠癌淋巴结转移动物模型的建立及其生物学特性[J]. 中国癌症杂志, 2016, 26(10): 840-847. DOI： 10.19401/j.cnki.1007-3639.2016.10.006.

孙轶群, 童　彤, 毛　健. Lymph node metastatic models of VX2 tumor in New Zealand white rabbits and their biological characteristics[J]. China Oncology, 2016, 26(10): 840-847. DOI： 10.19401/j.cnki.1007-3639.2016.10.006.

摘要

背景与目的：直肠癌淋巴结转移模型是研究肿瘤发生、发展、转移及抗肿瘤治疗的工具，但较大型动物模型鲜见报道。该研究旨在建立可行性强、重复率高的VX2移植性兔直肠癌淋巴结转移模型。方法：采用原位移植方法，将切好的小瘤块置入穿刺针内，并使用穿刺针将小瘤块推入到新西兰大白兔的直肠壁内。共制作模型20只。每周使用MR扫描2只实验兔，MR上观察肿瘤生长情况及肠周淋巴结个数，使用MR后处理软件测量实验兔的直肠壁肿瘤体积，扫描完成后进行详细解剖，切除直肠壁上肿瘤及肠周淋巴结，进行标本固定及HE染色，并探讨肿瘤体积与生长时间及转移淋巴结个数的关系。结果：成功制作模型13只，成功率为65%。于第4周开始在MR上可观察到局限于直肠壁肿块。随着时间增长，肿瘤体积不断增大，且不同时期(生长周数)肿瘤体积差异有统计学意义(F=52.865，P0.05)；进一步分析得出，肿瘤的平均体积与生长周数呈正线性相关(r=0.910)，差异有统计学意义(P0.05)。当肿瘤体积大于9 cm3时，实验兔开始出现转移性淋巴结，第9周开始转移淋巴结个数明显增多。统计分析得出肿瘤体积越大，转移性淋巴结个数就越多(F=92.531，P0.05)，且两者呈线性相关(r=0.945)，差异有统计学意义 (P0.05)。结论：本实验将组织学完整的VX2移植瘤组织块原位种植到新西兰大白兔直肠内，成功建立了VX2移植性兔直肠癌的淋巴结转移模型。本模型对于研究直肠癌的局部生长、浸润机制、淋巴结转移灶及生物学特性均有一定价值。

Abstract

Background and purpose: The lymph node metastatic model of rectal tumor is a useful tool for the research on tumor occurrence

development

metastasis and antineoplastic therapy. There are few reports about establishment of larger animal model. This study aimed to establish feasible and reproducible lymph node metastatic models of VX2 tumor in rabbits. Methods: The VX2 tumor tissue was put into the puncture needle. The VX2 tumor tissue in the needle was orthotopically transplanted into the rectal wall of the New Zealand white rabbits successfully. Twenty New Zealand white rabbits were transplanted. Two experimental rabbits were scanned by MR weekly. Tumor growth curve and lymph node numbers were observed on MR. Experimental rabbit tumor volumes were measured by MR post-processing software. The rectal tumor and surrounding lymph nodes were resected

and the specimens were fixed. The sections were stained with HE. We explored the relationship between tumor volume and growth time

the number of metastatic lymph nodes and tumor volume

respectively. Results: Thirteen models were successfully established with a rate of 65%. Tumors limited in the rectal wall were observed on the fourth week. Tumor size increased over time. There was significant difference in the tumor volume between different periods (growth cycle number) (F=52.865

P0.05). There was a significantly positive correlation between tumor volume and the growth cycle number (r=0.910

P0.05). The metastatic lymph nodes could be observed when VT9 cm3. The number of metastatic lymph node increased obviously from the ninth week. The more tumor volume

the greater the number of metastatic lymph nodes was observed (F=92.531

P0.05). There was a significantly positive correlation between the number of metastatic lymph nodes and the tumor volume (r=0.945

P0.05). Conclusion: Metastatic lymph node models of VX2 tumor in New Zealand white rabbits were established successfully. This model has some value in the research on local growth

invasion mechanism

lymph node metastasis and biological characteristics of rectal cancer.

关键词

Keywords

references

Views

2281

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

The predictive value and model establishment of body composition in the long-term prognosis of patients after rectal cancer surgery

Exploring innovative models of surgical treatment for rectal cancer

Analysis of quality of life of rectal cancer patients after transanal total mesorectal excision and laparoscopic total mesorectal excision: a retrospective cohort study

Relationship between quantitative analysis parameters of DCE-MRI and microangiogenesis in rectal cancer

Exploring the attenuating effect of amifostine on neoadjuvant radiotherapy with concurrent use of irinotecan for locally advanced rectal cancer: a retrospective cohort study of 154 cases

Related Author

Shuo LIU

Yun LU

Jilin HU

Yuan GAO

Yunzhi GAO

Zhaolin DU

Zheng MA

Zechen LU

Related Institution

Department of Gastrointestinal Surgery, Rizhao Traditional Chinese Medicine Hospital

Department of Gastrointestinal Surgery, The Affiliated Hospital of Qingdao University

Department of Colorectal Surgery, DivisionⅡ, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College, Fudan University

Department of General Surgery, Renhe Hospital, Shanghai University

Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University

AI问答

⁰