The research on apoptosis of human laryngeal cell line Hep-2 induced by 1,4-bis[2-(benzylselanyl) ethoxy] anthracene

李　莎; 张思楠; 秦大斌

doi:10.19401/j.cnki.1007-3639.2016.12.005

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The research on apoptosis of human laryngeal cell line Hep-2 induced by 1,4-bis[2-(benzylselanyl) ethoxy] anthracene

更新时间：2025-12-31

- The research on apoptosis of human laryngeal cell line Hep-2 induced by 1,4-bis[2-(benzylselanyl) ethoxy] anthracene
- China Oncology Vol. 26, Issue 12, Pages: 989-995(2016)
- 作者机构：
  
  1. 西华师范大学西南野生动植物资源保护省部共建教育部重点实验室,四川,南充,637009
  2. 西华师范大学化学化工学院,四川,南充,637009
- 作者简介：
- 基金信息：
- DOI：10.19401/j.cnki.1007-3639.2016.12.005
  CLC：
- Published Online：23 January 2017，
  
  Published：23 January 2016
- 稿件说明：
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李　莎,张思楠,秦大斌,等. 1,4-二(2-苄硒乙氧基)蒽诱导人喉癌Hep-2细胞凋亡的研究[J]. 中国癌症杂志, 2016, 26(12): 989-995.

李　莎, 张思楠, 秦大斌. The research on apoptosis of human laryngeal cell line Hep-2 induced by 1,4-bis[2-(benzylselanyl) ethoxy] anthracene[J]. China Oncology, 2016, 26(12): 989-995.
李　莎,张思楠,秦大斌,等. 1,4-二(2-苄硒乙氧基)蒽诱导人喉癌Hep-2细胞凋亡的研究[J]. 中国癌症杂志, 2016, 26(12): 989-995. DOI： 10.19401/j.cnki.1007-3639.2016.12.005.

李　莎, 张思楠, 秦大斌. The research on apoptosis of human laryngeal cell line Hep-2 induced by 1,4-bis[2-(benzylselanyl) ethoxy] anthracene[J]. China Oncology, 2016, 26(12): 989-995. DOI： 10.19401/j.cnki.1007-3639.2016.12.005.

摘要

背景与目的：硒是人类生命活动中必不可少的微量元素之一，对人类健康的巨大作用是其他物质无法替代的。硒的小分子化合物1

4-二(2-苄硒乙氧基)蒽{1

4-bis［2-(benzylselanyl)ethoxy］ anthracene，BSEA}具有杀菌消炎的作用，但BSEA的抗癌作用尚未见报道。该研究旨在探讨BSEA诱导人喉癌Hep-2细胞凋亡及其作用机制。方法：不同浓度的BSEA处理人喉癌Hep-2细胞24 h后，采用四甲基偶氮唑盐(methyl thiazolyl tetrazolium，MTT)法检测细胞生长抑制率；在荧光显微镜下观察细胞形态；Annexin Ⅴ-FITC法检测细胞凋亡；JC-1检测线粒体膜电位；酶标分析仪检测caspase-3和caspase-8活性；实时荧光定量聚合酶链反应(real-time fluorescent quantitative polymerase chain reaction，RTFQ-PCR)和蛋白［质］印迹法(Western blot)分别检测Bax、XIAPmRNA和蛋白表达水平。结果：BSEA处理人喉癌Hep-2细胞24 h后，BSEA对其生长抑制呈剂量依赖性，IC50为35.74 μmol/L；80 μmol/L BSEA处理的Hep-2细胞在荧光显微镜下可观察到明显的凋亡小体；磷脂酰丝氨酸外翻加剧；线粒体膜电位开始下降；caspase-3活性的影响呈剂量依赖趋势，而caspase-8活性未见显著变化；Bax的mRNA和蛋白表达水平上升，XIAP的mRNA和蛋白表达水平下降。结论：BSEA对人喉癌Hep-2细胞生长有明显的抑制作用，并能通过线粒体途径诱导细胞凋亡。

Abstract

Background and purpose: Selenium is one of the essential trace elements for human activities

and plays an incomparable role in maintaining human health. It was reported that selenium compound 1

4-bis［2-(benzylselanyl) ethoxy］ (BSEA) anthracene has antiseptic and antiphlogistic effects. However

the mechanisms underlying anticancer effects of BSEA are rarely reported. BSEA-induced apoptosis in human laryngeal carcinoma Hep-2 cells and its mechanisms were studied. Methods: Methyl thiazolyl tetrazolium (MTT) assay was used to determine inhibition ratio of Hep-2 cells 24 hours after Hep-2 cells were treated with different concentrations of BSEA. Fluorescence microscope was used to observe the morphology change of apoptosis in Hep-2 cells. The apoptosis was detected by Annexin Ⅴ-FITC. Mitochondrial membrane potential was assayed by JC-1. Microplate reader detected the activity of caspase-3 and caspase-8. The mRNA and protein levels of Bax and XIAP were measured by real-time fluorescent quantitative polymerase chain reaction (RTFQ-PCR) and Western blot. Results: The results showed that BSEA caused a dose-dependent inhibition of the growth of human laryngeal carcinoma cell line Hep-2 in vitro

and IC50 was 35.74 μmol/L. The apoptotic bodies were distinctly observed at a concentration of 80 μmol/L of BSEA by AO fluorescence staining. This study found that the eversion of phosphatidyl serine intensified

and mitochondrial membrane potential also began to decline. The activity of caspase-3 appeared the tendency of dependence on dosage

while the activity of caspase-8 did not change significantly. The mRNA and protein expression level of Bax increased

whereas the mRNA and protein expression level of XIAP decreased. Conclusion: Therefore

BSEA could obviously inhibit human laryngeal carcinoma Hep-2 cells proliferation and induce apoptosis via the mitochondrial pathway.

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