Establishment and identification of human placental site trophoblastic tumor cell line

刘齐雨; 李　可; 胥　婧

doi:10.19401/j.cnki.1007-3639.2017.07.001

您当前的位置：

首页 >

文章列表页 >

Establishment and identification of human placental site trophoblastic tumor cell line

更新时间：2025-12-31

- Establishment and identification of human placental site trophoblastic tumor cell line
- China Oncology Vol. 27, Issue 7, Pages: 521-526(2017)
- 作者机构：
  
  1. 复旦大学附属妇产科医院中西医结合科,上海,200011
  2. 上海市女性生殖内分泌相关疾病重点实验室,上海,200011
- 作者简介：
- 基金信息：
- DOI：10.19401/j.cnki.1007-3639.2017.07.001
  CLC：
- Published Online：16 August 2017，
  
  Published：16 August 2017
- 稿件说明：
移动端阅览
刘齐雨,李　可,胥　婧,等. 人胎盘部位滋养细胞肿瘤细胞系的建立及其鉴定[J]. 中国癌症杂志, 2017, 27(7): 521-526.

刘齐雨, 李　可, 胥　婧. Establishment and identification of human placental site trophoblastic tumor cell line[J]. China Oncology, 2017, 27(7): 521-526.
刘齐雨,李　可,胥　婧,等. 人胎盘部位滋养细胞肿瘤细胞系的建立及其鉴定[J]. 中国癌症杂志, 2017, 27(7): 521-526. DOI： 10.19401/j.cnki.1007-3639.2017.07.001.

刘齐雨, 李　可, 胥　婧. Establishment and identification of human placental site trophoblastic tumor cell line[J]. China Oncology, 2017, 27(7): 521-526. DOI： 10.19401/j.cnki.1007-3639.2017.07.001.

摘要

背景与目的：胎盘部位滋养细胞肿瘤(placental site trophoblastic tumor，PSTT)是一种特殊类型的妊娠滋养细胞肿瘤。该研究旨在建立人PSTT细胞系，鉴定其基本特性。方法：通过手术切除的PSTT组织标本原代培养，纯化并传代，建立人PSTT细胞系。鉴定方法包括：观察细胞形态，CCK-8法绘制细胞生长曲线，进行染色体核型分析，细胞与肿瘤组织短串重复序列(short tandem repeat，STR)分析，以及免疫组织化学染色。结果：建立稳定传代的人PSTT细胞系PSTT-1。PSTT-1细胞单层贴壁生长，失去接触抑制性，呈多突起的纺锤形结构。体外培养生长良好，每5 d传代1次，目前已传至30余代。染色体核型分析结果为正常女性核型：46，XX。PSTT-1与肿瘤组织STR分析结果高度匹配。PSTT-1细胞表达β-catenin、CD146、人绒毛膜促性腺激素(human chorionic gonadotrophin，hCG)、人胎盘生乳素(human placental lactogen，hPL)和Muc4。结论：该研究成功建立人PSTT细胞系PSTT-1，为PSTT的研究提供了体外实验模型。

Abstract

Background and purpose: Placental site trophoblastic tumor (PSTT) is a special type of gestational trophoblastic tumor. It remains no general cell line for PSTT. The aim of this study was to establish and identify a human PSTT cell line. Methods: In this study

a PSTT cell line (PSTT-1) was derived from primary culture of a surgically resected PSTT tissue. We further identified the basic characteristics of PSTT-1

including cell morphology

cell growth curve

karyotype analysis

short tandem repeat (STR) profiling of PSTT-1 and its parental tumor

and immunohistochemistry analysis. Results: A human PSTT cell line named PSTT-1 was successfully established. PSTT-1 formed a monolayer growth and lost contact inhibition

and exhibited a pleomorphic morphology. PSTT-1 cells grew actively in vitro. PSTT-1 was passaged every 5 days

and maintained in culture for more than 30 passages. Karyotyping of PSTT-1 revealed normal female karyotype: 46

XX. STR profiling of PSTT-1 was nearly identical to its parental tumor. Immunohistochemistry analysis of PSTT-1 revealed expression of β-catenin

CD146

human chorionic gonadotropin (hCG)

human placental lactogen (hPL) and Muc4. Conclusion: This study established a PSTT cell line

which could be useful for further research on PSTT.

关键词

Keywords

references

Views

1915

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Advances in preclinical research models for gastroenteropancreatic neuroendocrine neoplasm

Related Author

Lingjun CUI

Chao TIAN

Zixuan CHENG

Jiabin ZHENG

Fei SU

Huangying TAN

Related Institution

Graduate School, Beijing University of Chinese Medicine

Department of Integrative Oncology, China-Japan Friendship Hospital

AI问答

⁰