Effect of silencing LASP1 on proliferation, migration and invasion of human gastric cancer cell line BGC823

刘玉珍; 张梦雪; 吕世军

doi:10.19401/j.cnki.1007-3639.2017.11.002

您当前的位置：

首页 >

文章列表页 >

Effect of silencing LASP1 on proliferation, migration and invasion of human gastric cancer cell line BGC823

更新时间：2025-12-31

- Effect of silencing LASP1 on proliferation, migration and invasion of human gastric cancer cell line BGC823
- China Oncology Vol. 27, Issue 11, Pages: 847-852(2017)
- 作者机构：
  
  潍坊医学院临床病理系，神经疾病与再生修复实验室,山东,潍坊,261053
- 作者简介：
- 基金信息：
- DOI：10.19401/j.cnki.1007-3639.2017.11.002
  CLC：
- Published Online：12 December 2017，
  
  Published：12 December 2017
- 稿件说明：
移动端阅览
刘玉珍,张梦雪,吕世军,等. 干扰

刘玉珍, 张梦雪, 吕世军. Effect of silencing LASP1 on proliferation, migration and invasion of human gastric cancer cell line BGC823[J]. China Oncology, 2017, 27(11): 847-852.
刘玉珍,张梦雪,吕世军,等. 干扰 DOI： 10.19401/j.cnki.1007-3639.2017.11.002.

刘玉珍, 张梦雪, 吕世军. Effect of silencing LASP1 on proliferation, migration and invasion of human gastric cancer cell line BGC823[J]. China Oncology, 2017, 27(11): 847-852. DOI： 10.19401/j.cnki.1007-3639.2017.11.002.

摘要

背景与目的：LASP1是一种新的肌动蛋白结合蛋白，参与细胞骨架的重组调控及细胞迁移，在多种恶性肿瘤中高表达，并与肿瘤的增殖、侵袭和转移密切相关。但目前LASP1在胃癌发生、发展中的作用少见报道。该研究旨在探讨LASP1在胃癌增殖和侵袭中的作用及分子机制。方法：利用实时荧光定量聚合酶链反应(real-time fluorescent quantitative polymerase chain reaction，RTFQ-PCR)和蛋白［质］印迹法(Western blot)检测LASP1在不同胃癌细胞系中的表达，应用RNA干扰技术抑制BGC823细胞中LASP1的表达，采用RTFQ-PCR和Western blot检测转染后LASP1的表达，应用体外增殖、迁移和侵袭实验检测转染后细胞的增殖、迁移和侵袭能力，通过F-actin聚合实验检测细胞的F-actin的聚合能力，采用Western blot检测转染前后BGC823细胞中Akt的磷酸化情况。结果：LASP1在胃癌BGC823细胞中高表达。RNA干扰技术沉默LASP1基因后，干扰组与对照组相比，LASP1的mRNA和蛋白表达水平均明显下降。细胞增殖实验显示，干扰组细胞的增殖率低于对照组(P0.05)；Transwell迁移和侵袭实验发现，与对照组相比，干扰组细胞的迁移和侵袭能力均明显降低(P0.05)；在干扰组细胞内的F-actin聚合量比对照组减少(P0.05)；在干扰组细胞内Akt磷酸化受到抑制。结论：LASP1的表达降低可以抑制胃癌BGC823细胞的体外增殖、迁移和侵袭能力，LASP1通过调节F-actin的聚合和Akt磷酸化从而影响胃癌细胞的侵袭、转移。

Abstract

Background and purpose: LASP1 is a novel actin-binding protein and involved in the process of cytoskeleton reorganization and cell migration. Expression of LASP1 is significantly up-regulated in several malignant tumors and is closely related to the tumor proliferation

invasion and metastasis. However

the specific mechanism of LASP1 is not clear in gastric carcinogenesis. This study aimed to detect the effect and mechanism of LASP1 in the proliferation and invasion of gastric cancer cells. Methods: Real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR) and Western blot were employed to determine the expression of LASP1 in gastric cancer cell lines

as well as human normal gastric epithelial cell line GES-1. RNA interference (RNAi) was used to silence the expression of LASP1 in gastric cancer cell BGC823. RTFQ-PCR and Western blot were used to analyze the expression of LASP1 after siRNA transfection. In vitro proliferation

migration and invasion assay were performed to detect the effect of LASP1 on BGC823 cells. F-actin polymerization assay was used to detect F-actin recognition ability in cells. Western blot was used to analyze phosphorylation of Akt. Results: Overexpression of LASP1 was detected in human gastric cancer BGC823 cells. Down-regulated expression of LASP1 at both mRNA and protein level was detected after siRNA transfection. Cell proliferation assay showed that the growth rate was significantly reduced in RNAi group (P0.05). In transwell assay

the cell migration and invasion were significantly decreased (P0.05). F-actin content was reduced compared with the control group (P0.05). The phosphorylation of Akt was significantly reduced after siRNA transfection. Conclusion: LASP1 was highly expressed in gastric cancer cells. Down-regulation of LASP1 suppressed the capacities of proliferation

migration and invasion of human gastric cancer BGC823 cells in vitro. LASP1 can induce migration and invasion of gastric cancer through regulation of F-actin polymerization and Akt phosphorylation.

关键词

Keywords

references

Views

2328

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Mechanistic study on PLK4 regulation of invasion, proliferation and apoptosis in oral squamous cell carcinoma

PARP1 promotes the progression of hepatocellular carcinoma by regulating expression of POU2F2

Effects of TMCO1 on proliferation and migration of cervical cancer cells

CDC20 facilitates the proliferation of esophageal carcinoma cell by stabilizing NLRP3 expression

PRMT7 inhibits proliferation and migration of bladder cancer cells by regulating Notch signaling pathway

Related Author

Huan QIN

Jie WANG

Jie YANG

Yingjie HUA

Huijuan QU

Honghai JI

Ziqiang WEN

Junliang LAN

Related Institution

Department of Stomatology, Affiliated Hospital of Shandong Second Medical University

Shouguang Stomatological Hospital

School of Stomatology, Shandong Second Medical University

Department of Hepatobiliary Surgery, Linfen People’s Hospital, The Seventh Clinical Medical College of Shanxi Medical University

Department of Obstetrics and Gynecology, Zhongshan Hospital Affiliated to Xiamen University

AI问答

⁰