Effect of glioma-associated oncogene homolog 2 silencing on proliferation and apoptosis of colon cancer cell line SW620

KANG Qingjie; XIANG Zheng

doi:10.19401/j.cnki.1007-3639.2020.10.009

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Effect of glioma-associated oncogene homolog 2 silencing on proliferation and apoptosis of colon cancer cell line SW620

更新时间：2026-01-27

- Effect of glioma-associated oncogene homolog 2 silencing on proliferation and apoptosis of colon cancer cell line SW620
- China Oncology Vol. 30, Issue 10, Pages: 785-790(2020)
- 作者机构：
  
  重庆医科大学附属第一医院胃肠外科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.19401/j.cnki.1007-3639.2020.10.009
  CLC：
- Published Online：12 November 2020，
  
  Published：12 November 2020
- 稿件说明：
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康清杰, 向　征. 沉默胶质瘤相关癌基因同源物2基因对结肠癌细胞系SW620增殖和凋亡的影响[J]. 中国癌症杂志, 2020, 30(10): 785-790.

KANG Qingjie, XIANG Zheng. Effect of glioma-associated oncogene homolog 2 silencing on proliferation and apoptosis of colon cancer cell line SW620[J]. China Oncology, 2020, 30(10): 785-790.
康清杰, 向　征. 沉默胶质瘤相关癌基因同源物2基因对结肠癌细胞系SW620增殖和凋亡的影响[J]. 中国癌症杂志, 2020, 30(10): 785-790. DOI： 10.19401/j.cnki.1007-3639.2020.10.009.

KANG Qingjie, XIANG Zheng. Effect of glioma-associated oncogene homolog 2 silencing on proliferation and apoptosis of colon cancer cell line SW620[J]. China Oncology, 2020, 30(10): 785-790. DOI： 10.19401/j.cnki.1007-3639.2020.10.009.

摘要

背景与目的：胶质瘤相关癌基因同源物2（glioma-associated oncogene homolog 2，Gli2）是Hedgehog 信号通路重要的转录因子，它不仅与正常细胞的生长密切相关，而且在多种肿瘤细胞中异常激活。探讨Gli2对结肠癌细胞系SW620增殖和凋亡的影响及其可能机制。方法：以Gli2干扰慢病毒载体感染SW620细胞，实验设干扰组、空病毒载体组和对照组，采用实时荧光定量聚合酶链反应（real-time fluorescence quantitative polymerase chain reaction，RTFQ-PCR）和蛋白［质］印迹法（Western blot）检测细胞中Gli2 mRNA表达和蛋白水平，采用细胞计数试剂盒-8（cell counting kit-8，CCK-8）、集落形成实验和倍增时间检测细胞的增殖情况，流式细胞术（flow cytometry）检测各组细胞周期和凋亡情况，Western blot法检测细胞中ERK1/2、p-ERK1/2、Bcl-2、Bax和cyclin D1蛋白水平。结果：SW620细胞转染慢病毒载体72 h后，可见明显的荧光表达；与空病毒载体组和对照组相比，干扰组细胞的Gli2基因表达被有效抑制（P0.05）；细胞增殖能力明显降低（P0.05）；细胞周期阻滞，G

期细胞比例增高（P0.05）；细胞凋亡率明显增加（P0.05）；p-ERK1/2、Bcl-2和cyclin D1的蛋白表达量降低（P0.05），Bax表达增加（P0.05）。结论：沉默Gli2可以明显抑制结肠癌细胞系SW620的增殖，促进细胞凋亡，其机制可能与p-ERK1/2 、Bcl-2和cyclin D1表达的下调和Bax表达上调相关。

Abstract

Background and purpose: Glioma-associated oncogene homolog 2 (Gli2) is an important transcription factor of Hedgehog signaling pathway

which is closely related to not only the growth of normal cells

but also abnormal activation in a variety of tumor cells. This study aimed to explore the effect of Gli2 on the proliferation and apoptosis of colon cancer cell line SW620 and its possible mechanism. Methods: The SW620

cells were infected with Gli2 interference lentivirus. The expressions of Gli2 mRNA and protein were confirmed by real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR) and Western blot. Cell proliferation was detected by cell counting kit-8 (CCK-8) assay

colony formation assay and doubling time. Flow cytometry was used to detect the cell cycle and apoptosis of SW620. Western blot was used to detect the protein expressions of ERK1/2

p-ERK1/2

Bcl-2

Bax and cyclin D1. Results: After infection of SW620 with Gli2 interference lentivirus for 72 h

significant fluorescence expression was seen. Compared with the empty vector group and the control group

the expression of Gli2 in interference group was effectively inhibited (P0.05); cell proliferation was significantly reduced (P0.05); the cell cycle was arrested

and G

phase cell proportion was increased (P0.05); the apoptotic rate was significantly increased (P0.05); the protein expressions of ERK1/2

p-ERK1/2

Bcl-2 and cyclin D1 were decreased (P0.05)

and the expression of Bax was increased (P0.05). Conclusion: The siRNA-mediated Gli2 silencing significantly inhibited the proliferation of colon cancer cell line SW620 and promoted apoptosis

which may be related to the down-regulation of p-ERK1/2

Bcl-2 and cyclin D1 expressions and up-regulation of Bax expression.

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