Expression and effect of long non-coding RNA ARAP1-AS1 in clear cell renal cell carcinoma

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Expression and effect of long non-coding RNA ARAP1-AS1 in clear cell renal cell carcinoma

Article | 更新时间：2025-12-31

- Expression and effect of long non-coding RNA ARAP1-AS1 in clear cell renal cell carcinoma
- China Oncology Vol. 32, Issue 1, Pages: 34-40(2022)
- 作者机构：
  
  1. 南昌大学第一附属医院泌尿外科,江西南昌 330006
  2. 南昌大学第一附属医院病理科,江西南昌 330006
- 作者简介：
- 基金信息：
- DOI：10.19401/j.cnki.1007-3639.2022.01.004
  CLC： R737.11
- Received：07 October 2021，
  
  Revised：2021-11-25，
  
  Published：30 January 2022
- 稿件说明：
移动端阅览
Kunyang LEI, Wenjie XIE, Ting SUN, et al. Expression and effect of long non-coding RNA ARAP1-AS1 in clear cell renal cell carcinoma[J]. China Oncology, 2022, 32(1): 34-40.
DOI：

Kunyang LEI, Wenjie XIE, Ting SUN, et al. Expression and effect of long non-coding RNA ARAP1-AS1 in clear cell renal cell carcinoma[J]. China Oncology, 2022, 32(1): 34-40. DOI： 10.19401/j.cnki.1007-3639.2022.01.004.

摘要

背景与目的：

长链非编码RNA（long non-coding RNA

lncRNA）ARAP1-AS1在多种肿瘤中异常表达

但其在肾

透明细胞癌（clear cell renal cell carcinoma

ccRCC）中的作用尚不清楚。探讨ARAP1-AS1在ccRCC中的生物学作用。

方法：

通过GEPIA数据库分析ARAP1-AS1在ccRCC组织中的表达及其与临床病理学特征及患者生存率的关系。采用实时荧光定量聚合酶链反应（real-time fluorescence quantitative polymerase chain reaction

RTFQ-PCR）检测ccRCC组织及邻近的非肿瘤组织中ARAP1-AS1的表达水平。将患者分为ARAP1-AS1高表达组和低表达组

分析ARAP1-AS1的表达水平与患者临床病理学特征之间的关系

并进行生存分析。通过细胞计数试剂盒-8（cell counting kit-8

CCK-8）实验、transwell迁移实验及侵袭实验检测ARAP1-AS1对ccRCC细胞体外增殖、迁移及侵袭能力的影响。采用蛋白质印迹法（Western blot）检测Wnt/

&

#x003b2;-catenin信号通路相关蛋白表达变化。采用BALB/c裸小鼠移植瘤模型分析ARAP1-AS1对ccRCC细胞体内成瘤能力的影响。

结果：

GEPIA数据库分析结果显示

ARAP1-AS1在ccRCC中高表达

且与患者肿瘤高分期及较差的生存率相关（

P

均

<

0.05）。RTFQ-PCR显示

ARAP1-AS1在ccRCC组织及细胞系中高表达

ARAP1-AS1的高表达与肿瘤大小和分期相关（

P

均

<

0.05）。ARAP1-AS1高表达患者的总生存率较差（

P

<

0.05）。沉默ARAP1-AS1的表达可以抑制ccRCC细胞增殖、迁移和侵袭（

P

均

<

0.05）。沉默ARAP1-AS1可以降低Wnt/

&

#x003b2;-catenin信号通路相关蛋白的表达水平（

P

均

<

0.05）。沉默ARAP1-AS1可使ccRCC细胞体内成瘤能力减弱

并使Ki-67增殖指数降低。

结论：

ARAP1-AS1可通过激活Wnt/

&

#x003b2;-catenin信号通路促进ccRCC的进展。

Abstract

Background and purpose:

Long non-coding RNA (lncRNA) ARAP1-AS1 is abnormally expressed in a variety of tumors

but its role in clear cell renal cell carcinoma (ccRCC) is unknown. This study aimed to explore the biological role of ARAP1-AS1 in ccRCC.

Methods:

The expression level of ARAP1-AS1 in ccRCC tissues and its relationship to patient

&

#x02019;s clinicopathological characteristics and survival rate were analyzed using the GEPIA database. The expression level of ARAP1-AS1 was measured in ccRCC and adjacent non-tumor tissues by real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR). Patients were divided into ARAP1-AS1 high and low expression groups

the relationship between ARAP1-AS1 expression level and patient

&

#x02019;s clinicopathological characteristics was analyzed

and survival analysis was performed. The effect of ARAP1-AS1

in vitro

proli

feration

migration and invasion ability of ccRCC cells was determined by cell counting kit-8 (CCK-8) assay

transwell migration and invasion assay. Changes in the expressions of Wnt/

&

#x003b2;-catenin signaling pathway-related proteins were detected by Western blot assay. The effect of ARAP1-AS1 on tumorigenic capacity in ccRCC cells

in vivo

was verified by tumor xenografts in nude mice.

Results:

The analysis of the GEPIA database showed that ARAP1-AS1 was highly expressed in ccRCC and associated with advanced tumor stage as well as poor survival in patients (all

P

<

0.05). The results of RTFQ-PCR showed that ARAP1-AS1 was highly expressed in ccRCC tissues and cell lines

and high expression correlated with tumor size and stage (all

P

<

0.05). The overall survival rate was poor in patients with high ARAP1-AS1 expression (

P

<

0.05). Knockdown of ARAP1-AS1 expression inhibited the proliferation

migration and invasion of ccRCC cells (all

P

<

0.05). Silencing of ARAP1-AS1 reduced the expression levels of the proteins involved in the Wnt/

&

#x003b2;-catenin signaling pathway (all

P

<

0.05). Silencing of ARAP1-AS1 attenuated tumorigenic capacity in ccRCC cells and reduced Ki-67 proliferation index (

P

<

0.05).

Conclusion:

ARAP1-AS1 promotes ccRCC progression through activation of the Wnt/

&

#x003b2;-catenin signaling pathway.

关键词

Keywords

references

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