miR-22 inhibited glioma cells proliferation by targeting MTDH

李荣国; 王剑; 杨少陵

doi:10.3969/j.issn.1007-3969.2014.06.001

您当前的位置：

首页 >

文章列表页 >

miR-22 inhibited glioma cells proliferation by targeting MTDH

更新时间：2025-12-31

- miR-22 inhibited glioma cells proliferation by targeting MTDH
- China Oncology Vol. 24, Issue 6, Pages: 401-405(2014)
- 作者机构：
  
  南华大学附属第一医院急诊科,湖南,衡阳,421001
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1007-3969.2014.06.001
  CLC：
- Published Online：29 July 2014，
  
  Published：29 July 2014
- 稿件说明：
移动端阅览
李荣国,王剑,杨少陵. miR-22通过靶向MTDH抑制胶质瘤细胞的生长[J]. 中国癌症杂志, 2014, 24(6): 401-405.

李荣国, 王剑, 杨少陵. miR-22 inhibited glioma cells proliferation by targeting MTDH[J]. China Oncology, 2014, 24(6): 401-405.
李荣国,王剑,杨少陵. miR-22通过靶向MTDH抑制胶质瘤细胞的生长[J]. 中国癌症杂志, 2014, 24(6): 401-405. DOI： 10.3969/j.issn.1007-3969.2014.06.001.

李荣国, 王剑, 杨少陵. miR-22 inhibited glioma cells proliferation by targeting MTDH[J]. China Oncology, 2014, 24(6): 401-405. DOI： 10.3969/j.issn.1007-3969.2014.06.001.

摘要

背景与目的：miR-22在胃癌、肺癌、结肠癌以及乳腺癌中表达下调，然而其在胶质瘤中的表达情况尚未明确。本研究旨在探讨miR-22是否通过靶向调控MTDH表达抑制胶质瘤细胞生长，从而进一步揭示miR-22的抑瘤机制。方法：运用实时定量PCR(quantitative real-time polymerase chain reaction，qRT-PCR)检测75例胶质瘤及17例正常大脑组织中miR-22的表达改变以及与胶质瘤患者预后关系；构建MTDH 3’UTR-荧光素酶报告载体，通过荧光素酶报告检测观察miR-22对MTDH 3’UTR-荧光素酶活性的影响；将miR-22 mimics转染胶质瘤细胞U251，以MTDH siRNA为阳性对照，采用蛋白质印迹法(Western blot)检测其对MTDH蛋白表达水平的影响；然后采用MTT法检测高表达miR-22对U251细胞生长的影响。结果：qRT-PCR检测结果显示，miR-22在75例胶质瘤组织中表达下调；Kaplan-Meier生存分析发现，miR-22表达越高，患者生存率越高(P0.05)；在双荧光素酶报告检测显示，miR-22能特异性地与MTDH 3’UTR结合，抑制其荧光素酶活性。Western blot检测结果显示，miR-22过表达或干扰MTDH可抑制MTDH蛋白的表达。MTT检测发现，miR-22过表达或干扰MTDH可抑制人U251细胞的生长，差异有统计学意义(P0.05)。结论：miR-22通过靶向调控MTDH的表达而抑制胶质瘤细胞的生长。

Abstract

Background and purpose: miR-22 has been reported to be down-regulated in gastric cancer

lung cancer

colorectal cancer

and breast cancer. However

its expression in glioma was still poorly known. This study aimed to explicit whether miR-22 suppresses cell proliferation by targeting MTDH

thus to reveal molecular mechanism that miR-22 functions as a tumor suppressor in glioma. Methods: Quantitative real-time polymerase chain reaction (qRTPCR) was conducted for detecting the expression of miR-22 in gliomas and normal brain tissues. MTDH 3’UTRluciferase vector was constructed and dual-luciferase reporter gene assay was employed to examine the effect of miR-22 on luciferase activity. U251 cells were transfected with miR-22 mimics

and MTDH siRNA as for postive control

then Western blot was performed to detect the expressions of MTDH protein. The proliferation ability of U251 cells was evaluated by MTT assay. Results: miR-22 was down-regulated in glioma tissues. Glioma patients with relatively high expression of miR-22 showed lower mortality compared with low expression of miR-22 by using Kaplan-Meier survival curves. We demonstrated miR-22 could bind to the 3’ untranslated region (UTR) of MTDH and inhibited the luciferase activity. Western blot showed that the expression of MTDH protein was inhibited by restored miR-22 or siR MTDH in U251 cells. Overexpression of miR-22 or siR MTDH inhibited the proliferation of U251 cells. Conclusion: miR-22 suppresses cell proliferation by targeting MTDH in glioma.

关键词

Keywords

references

Views

105839

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Retrospective study on MGMT methylation status and its clinical significance in gliomas

WTAP promotes proliferation and aerobic glycolysis via regulating m6A modification of BMI1 mRNA

Screening recurrent glioblastoma-related genes and analyzing their gene expressions in association with clinicopathological parameters and prognosis

Effect ofCHKAgene silencing on biological behavior of glioma cell and its mechanism

The prognostic value of microfluidic core technology in detecting circulating tumor cells in brain glioma

Related Author

Qiying LIU

Xiaoli ZHU

Qianming BAI

Min REN

Tian XUE

Heng CHANG

Xiaoyan ZHOU

Jing ZHANG

Related Institution

Department of Pathology, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College, Fudan University; Fudan University Cancer Institute

Department of Neurosurgery, Wu’an First People’s Hospital

Department of Neurosurgery, the First Affiliated Hospital of Nanyang Medical College

Department of Neuro-oncology, Cancer Center, Beijing Tiantan Hospital, Capital Medical University

宁夏医科大学总医院神经外科

AI问答

⁰