Background and purpose: p53 binding protein (53BP1) expresses in many normal and tumor cells. In vitro experiments have confirmed that inhibition of the protein expression of 53BP1 can effectively eliminate cycle arrest of tumor cells

and increase the radiosensitivity after irradiation. However

the in vivo experiment has not been reported. This study aimed to explore the effect of silenc

ing 53BP1 gene on the growth and radiosensitivity to esophageal cancer cell ECA109 xenograft in nude mice. Methods: Forty-eight male BALB/c/nu nude mice were randomly divided into 6 groups: ECA109

ECA109/R

ECA109/N

ECA109/NR

ECA109/B and ECA109/BR. Three kinds of prepared cells (ECA109

ECA109/N and ECA109/B) were subcutaneously injected into the paw pads of mice (2×10

6

/100 μL per mouse). The nude mice in ECA109/R

ECA109/NR

and ECA109/BR groups were irradiated with 15 Gy. Tumor growth was monitored every other day on the 6

th

day after injection. Tumor volume was measured with calipers. The expression levels of CHK1

CHK2 and phosphorylated CHK2-T68 protein were examined in different groups by Western blot. Apoptotic cell and cell cycle distribution were detected by flow cytometry assay. Results: Visible tumors were detectable by day 7 after implantation

and the tumor volumes showed no significant differences among all the groups (F=0.67

P=0.69). After irradiation with 15 Gy

tumor volumes in ECA109/BR group were smaller than those in other groups (P=0.03); the growth inhibition rate increased

but the relative growth rate decreased significantly (P=0.01). The q value which reflected the radiosensitizing effect in ECA109/BR group was 1.45. The expressions of CHK1 and CHK2 at protein level in ECA109/BR group were not influenced (P=0.71). However

the level of phosphorylated CHK2-T68 expression decreased significantly after irradiation with 15 Gy (P=0.03). Cell cycle distribution and apoptosis were not significantly different among all the groups (P=0.45). Conclusion: Silencing 53BP1 gene expression could inhibit the growth of esophageal cancer cell xenograft and increase the radiosensitivity to tumors in the nude mice.