长链非编码RNA ARAP1-AS1在肾透明细胞癌中的表达及作用研究

雷坤阳; 谢文杰; 孙庭; 刘贻富; 王旭

doi:10.19401/j.cnki.1007-3639.2022.01.004

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长链非编码RNA ARAP1-AS1在肾透明细胞癌中的表达及作用研究

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- 长链非编码RNA ARAP1-AS1在肾透明细胞癌中的表达及作用研究
- Expression and effect of long non-coding RNA ARAP1-AS1 in clear cell renal cell carcinoma
- 中国癌症杂志 2022年32卷第1期页码：34-40
- 作者机构：
  
  1. 南昌大学第一附属医院泌尿外科,江西南昌 330006
  2. 南昌大学第一附属医院病理科,江西南昌 330006
- 作者简介：
  
  谢文杰 E-mail: doctorxiewenjie@163.com
- 基金信息：
  
  江西省卫生健康委科技计划项目(202210397)
- DOI：10.19401/j.cnki.1007-3639.2022.01.004
  中图分类号： R737.11
- 收稿：2021-10-07，
  
  修回：2021-11-25，
  
  纸质出版：2022-01-30
- 稿件说明：
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雷坤阳, 谢文杰, 孙庭, 等. 长链非编码RNA ARAP1-AS1在肾透明细胞癌中的表达及作用研究[J]. 中国癌症杂志, 2022,32(1):34-40.

Kunyang LEI, Wenjie XIE, Ting SUN, et al. Expression and effect of long non-coding RNA ARAP1-AS1 in clear cell renal cell carcinoma[J]. China Oncology, 2022, 32(1): 34-40.
雷坤阳, 谢文杰, 孙庭, 等. 长链非编码RNA ARAP1-AS1在肾透明细胞癌中的表达及作用研究[J]. 中国癌症杂志, 2022,32(1):34-40. DOI： 10.19401/j.cnki.1007-3639.2022.01.004.

Kunyang LEI, Wenjie XIE, Ting SUN, et al. Expression and effect of long non-coding RNA ARAP1-AS1 in clear cell renal cell carcinoma[J]. China Oncology, 2022, 32(1): 34-40. DOI： 10.19401/j.cnki.1007-3639.2022.01.004.

摘要

背景与目的：

长链非编码RNA（long non-coding RNA

lncRNA）ARAP1-AS1在多种肿瘤中异常表达

但其在肾

透明细胞癌（clear cell renal cell carcinoma

ccRCC）中的作用尚不清楚。探讨ARAP1-AS1在ccRCC中的生物学作用。

方法：

通过GEPIA数据库分析ARAP1-AS1在ccRCC组织中的表达及其与临床病理学特征及患者生存率的关系。采用实时荧光定量聚合酶链反应（real-time fluorescence quantitative polymerase chain reaction

RTFQ-PCR）检测ccRCC组织及邻近的非肿瘤组织中ARAP1-AS1的表达水平。将患者分为ARAP1-AS1高表达组和低表达组

分析ARAP1-AS1的表达水平与患者临床病理学特征之间的关系

并进行生存分析。通过细胞计数试剂盒-8（cell counting kit-8

CCK-8）实验、transwell迁移实验及侵袭实验检测ARAP1-AS1对ccRCC细胞体外增殖、迁移及侵袭能力的影响。采用蛋白质印迹法（Western blot）检测Wnt/

#x003b2;-catenin信号通路相关蛋白表达变化。采用BALB/c裸小鼠移植瘤模型分析ARAP1-AS1对ccRCC细胞体内成瘤能力的影响。

结果：

GEPIA数据库分析结果显示

ARAP1-AS1在ccRCC中高表达

且与患者肿瘤高分期及较差的生存率相关（

均

0.05）。RTFQ-PCR显示

ARAP1-AS1在ccRCC组织及细胞系中高表达

ARAP1-AS1的高表达与肿瘤大小和分期相关（

均

0.05）。ARAP1-AS1高表达患者的总生存率较差（

0.05）。沉默ARAP1-AS1的表达可以抑制ccRCC细胞增殖、迁移和侵袭（

均

0.05）。沉默ARAP1-AS1可以降低Wnt/

#x003b2;-catenin信号通路相关蛋白的表达水平（

均

0.05）。沉默ARAP1-AS1可使ccRCC细胞体内成瘤能力减弱

并使Ki-67增殖指数降低。

结论：

ARAP1-AS1可通过激活Wnt/

#x003b2;-catenin信号通路促进ccRCC的进展。

Abstract

Background and purpose:

Long non-coding RNA (lncRNA) ARAP1-AS1 is abnormally expressed in a variety of tumors

but its role in clear cell renal cell carcinoma (ccRCC) is unknown. This study aimed to explore the biological role of ARAP1-AS1 in ccRCC.

Methods:

The expression level of ARAP1-AS1 in ccRCC tissues and its relationship to patient

#x02019;s clinicopathological characteristics and survival rate were analyzed using the GEPIA database. The expression level of ARAP1-AS1 was measured in ccRCC and adjacent non-tumor tissues by real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR). Patients were divided into ARAP1-AS1 high and low expression groups

the relationship between ARAP1-AS1 expression level and patient

#x02019;s clinicopathological characteristics was analyzed

and survival analysis was performed. The effect of ARAP1-AS1

in vitro

proli

feration

migration and invasion ability of ccRCC cells was determined by cell counting kit-8 (CCK-8) assay

transwell migration and invasion assay. Changes in the expressions of Wnt/

#x003b2;-catenin signaling pathway-related proteins were detected by Western blot assay. The effect of ARAP1-AS1 on tumorigenic capacity in ccRCC cells

in vivo

was verified by tumor xenografts in nude mice.

Results:

The analysis of the GEPIA database showed that ARAP1-AS1 was highly expressed in ccRCC and associated with advanced tumor stage as well as poor survival in patients (all

0.05). The results of RTFQ-PCR showed that ARAP1-AS1 was highly expressed in ccRCC tissues and cell lines

and high expression correlated with tumor size and stage (all

0.05). The overall survival rate was poor in patients with high ARAP1-AS1 expression (

0.05). Knockdown of ARAP1-AS1 expression inhibited the proliferation

migration and invasion of ccRCC cells (all

0.05). Silencing of ARAP1-AS1 reduced the expression levels of the proteins involved in the Wnt/

#x003b2;-catenin signaling pathway (all

0.05). Silencing of ARAP1-AS1 attenuated tumorigenic capacity in ccRCC cells and reduced Ki-67 proliferation index (

0.05).

Conclusion:

ARAP1-AS1 promotes ccRCC progression through activation of the Wnt/

#x003b2;-catenin signaling pathway.

关键词

Keywords

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