不同级别子宫颈病变与lncRNA HOTTIP和H19单核苷酸多态性的关系研究

王春桃; 葛安兴; 吴红雁; 张学艳; 杨圣; 袁红香; 程艳萍; 冯延璐; 陆欣苑; 梁戈玉

doi:10.19401/j.cnki.1007-3639.2022.04.005

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不同级别子宫颈病变与lncRNA HOTTIP和H19单核苷酸多态性的关系研究

论著 | 更新时间：2025-12-31

- 不同级别子宫颈病变与lncRNA HOTTIP和H19单核苷酸多态性的关系研究
- The association between cervical lesions of different grades and lncRNA HOTTIP and H19 single nucleotide polymorphisms
- 中国癌症杂志 2022年32卷第4期页码：324-334
- 作者机构：
  
  1. 江苏医药职业学院,江苏盐城 224001
  2. 环境医学工程教育部重点实验室,东南大学公共卫生学院,江苏南京 210009
  3. 盐城市第一人民医院放疗科,江苏盐城 224001
- 作者简介：
  
  [ "王春桃（ORCID: 0000-0002-6391-3304）,硕士,副教授,江苏医药职业学院科技处副处长 E-mail: yammy_taotao@163.com" ]
  梁戈玉（ORCID: 0000-0002-5035-3088）,博士,教授,东南大学人事处副处长 E-mail: gyliang@seu.edu.cn
- 基金信息：
  
  国家自然科学基金(81673132);国家自然科学基金(81972998);江苏省卫健委项目(Z2018043);江苏省青蓝工程(2019);江苏省高职院校教师专业带头人高端研修项目资助(2021GRFX030);校级自然科学基金(20204103)
- DOI：10.19401/j.cnki.1007-3639.2022.04.005
  中图分类号： R737.33
- 收稿：2021-08-18，
  
  修回：2022-03-02，
  
  纸质出版：2022-04-30
- 稿件说明：
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王春桃, 葛安兴, 吴红雁, 等. 不同级别子宫颈病变与lncRNA HOTTIP和H19单核苷酸多态性的关系研究[J]. 中国癌症杂志, 2022,32(4):324-334.

Chuntao WANG, Anxing GE, Hongyan WU, et al. The association between cervical lesions of different grades and lncRNA HOTTIP and H19 single nucleotide polymorphisms[J]. China Oncology, 2022, 32(4): 324-334.
王春桃, 葛安兴, 吴红雁, 等. 不同级别子宫颈病变与lncRNA HOTTIP和H19单核苷酸多态性的关系研究[J]. 中国癌症杂志, 2022,32(4):324-334. DOI： 10.19401/j.cnki.1007-3639.2022.04.005.

Chuntao WANG, Anxing GE, Hongyan WU, et al. The association between cervical lesions of different grades and lncRNA HOTTIP and H19 single nucleotide polymorphisms[J]. China Oncology, 2022, 32(4): 324-334. DOI： 10.19401/j.cnki.1007-3639.2022.04.005.

摘要

背景与目的：

HOTTIP和H19都是lncRNA（long non-coding RNA

lncRNA）中的一员

在肿瘤的发生、发展和迁移过程中发挥重要作用。本研究探讨HOTTIP rs2067087、H19 rs2839698和H19 rs2107425单核苷酸多态性（single nucleotide polymorphism

SNP）与

不同级别子宫颈病变发病风险的关系。

方法：

收集2018年11月

#x02014;2020年12月盐城市第一人民医院收治的345例子宫颈病变患者的外周静脉血及相关临床资料

其中子宫颈上皮内瘤变（cervical intraepithelial neoplasia

CIN）220例

子宫颈癌（cervical cancer

CC）125例

选取同期住院的360例无子宫颈病变患者为对照。采用TaqMan实时荧光定量聚合酶链反应（real-time fluorescence quantitative polymerase chain reaction

RTFQ-PCR）方法对2组人群中的

HOTTIP

基因的SNP位点rs2067087和

19基因的SNP位点rs2839698、rs2107425进行基因分型

采用

检验和非条件logistic回归分析3个SNP位点与不同级别子宫颈病变的相关性。

结果：

与对照组相比

HOTTIP

rs2067087和

19 rs2839698在子宫颈病变患者中的基因型分布存在显著差异。在隐性模型中

携带

HOTTIP

rs2067087 GG基因型患者发生子宫颈病变的频率增加了52.2%（95% CI：1.021 ~ 2.269

0.05）

分层分析发现

该位点GG基因型主要增加了CIN的发病风险（OR=1.730

95% CI：1.117 ~ 2.680

0.05）;与CC+CT基因型相比

携带

19 rs2839698 TT基因型患者发生子宫颈癌的风险减少了70.8%（95% CI：0.087 ~ 0.976

0.05）。未发现H19 rs2107425位点与不同级别子宫颈病变相关（

0.05）。

结论：

HOTTIP

基因SNP位点rs2067087与不同级别子宫颈病变相关

其GG基因型可能是宫颈上皮内瘤变发生的危险因素;

19基因SNP位点rs2839698与子宫颈癌发病风险相关

其TT基因型可能是子宫颈癌发生的保护因素。

Abstract

Background and purpose:

Both HOTTIP and H19 are members of long non-coding RNA(lncRNA). They have been found to play an important role in the occurrence

development and migration of tumors. This study aimed to investigate the association between single nucleotide polymorphisms (SNP) of HOTTIP rs2067087

H19 rs2839698 and H19 rs2107425 and the risk of cervical lesions of different grades.

Methods:

The peripheral venous blood and related clinical data of 345 cases of cervical lesions were collected from Nov. 2018 to Dec. 2020 in Yancheng No.1 People's Hospital

including 220 cases of cervical intraepithelial neoplasia (CIN) and 125 cases of cervical cancer (CC). Three hundred and sixty patients without cervical lesions hospitalized during the same period were selected as the co

ntrol. TaqMan real-time fluorescence quantitative polymerase chain reaction (RTFQPCR) was used to genotype the HOTTIP SNP rs2067087

H19 SNP rs2839698 and rs2107425 in the two groups

and

test and unconditional logistic regression were used to analyze the association of the three SNP loci with cervical lesions of different grades.

Results:

Compared with the control group

there were significant differences in genotype distribution of HOTTIP rs2067087 and H19 rs2839698 in patients with cervical lesions. In the recessive model

the frequency of cervical lesions in patients with HOTTIP rs2067087 GG genotype increased by 52.2% (95% CI: 1.021-2.269

0.05). Stratified analysis showed that GG genotype at this locus mainly increased the risk of CIN (OR =1.730

95% CI: 1.117-2.680

0.05); Stratified analysis showed that the GG genotype at this locus mainly increased the risk of CIN (OR=1.730

95% CI:1.117-2.680

0.05). Compared with CC+CT genotype

the risk of cervical cancer in patients with H19 rs2839698 TT genotype was reduced by 70.8% (95% CI: 0.087-0.976

0.05). No correlation was found between H19 rs2107425 and cervical lesions of different grades (

0.05).

Conclusion:

HOTTIP rs2067087 SNP is associated with different levels of cervical lesions

and the GG genotype may be a risk factor for CIN; H19 rs2839698 SNP is associated with the risk of cervical cancer

and the TT genotype may be a protective factor for cervical cancer.

关键词

Keywords

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