T-G-C纳米材料联合声动力学治疗喉癌的研究

郑丹苹; 钱炜; 郑楚杰

doi:10.19401/j.cnki.1007-3639.2022.05.009

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T-G-C纳米材料联合声动力学治疗喉癌的研究

论著 | 更新时间：2025-12-31

- T-G-C纳米材料联合声动力学治疗喉癌的研究
- A study on T-G-C nanomaterials combined with sonodynamic therapy in the treatment of laryngeal cancer
- 中国癌症杂志 2022年32卷第5期页码：436-444
- 作者机构：
  
  1. 江苏大学附属人民医院耳鼻咽喉科,江苏镇江 212000
  2. 南京中医药大学附属常州市中医院耳鼻咽喉科,江苏常州 21300
- 作者简介：
  
  [ "郑丹苹（ORCID: 0000-0002-0215-8237）,硕士在读 E-mail: ncuzdp@163.com" ]
  钱炜（ORCID: 0000-0002-2385-7029）,博士,教授,江苏大学附属人民医院（临床医学院）副院长,江苏省特聘医学专家E-mail: dwqian@yahoo.com
- 基金信息：
- DOI：10.19401/j.cnki.1007-3639.2022.05.009
  中图分类号： R739.65
- 收稿：2022-02-14，
  
  修回：2022-05-03，
  
  纸质出版：2022-05-30
- 稿件说明：
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郑丹苹, 钱炜, 郑楚杰. T-G-C纳米材料联合声动力学治疗喉癌的研究[J]. 中国癌症杂志, 2022,32(5):436-444.

Danping ZHENG, Wei QIAN, Chujie ZHENG. A study on T-G-C nanomaterials combined with sonodynamic therapy in the treatment of laryngeal cancer[J]. China Oncology, 2022, 32(5): 436-444.
郑丹苹, 钱炜, 郑楚杰. T-G-C纳米材料联合声动力学治疗喉癌的研究[J]. 中国癌症杂志, 2022,32(5):436-444. DOI： 10.19401/j.cnki.1007-3639.2022.05.009.

Danping ZHENG, Wei QIAN, Chujie ZHENG. A study on T-G-C nanomaterials combined with sonodynamic therapy in the treatment of laryngeal cancer[J]. China Oncology, 2022, 32(5): 436-444. DOI： 10.19401/j.cnki.1007-3639.2022.05.009.

摘要

背景与目的：

喉癌是目前难治性的耳鼻咽喉科恶性肿瘤

常采用放化疗联合手术治疗

但该

疗法对患者的5年生存率和死亡率改善不大

且常伴随喉部功能丧失和药物抵抗。本研究旨在探索纳米声敏剂T-G-C颗粒介导的声动力学治疗（sonodynamic therapy

SDT）在抑制人喉癌细胞中的作用

为喉癌的治疗提供一种新型非侵入性的治疗方法。

方法：

基于已经合成的T-G-C纳米材料

在激光扫描共聚焦显微镜下评价Tu686细胞摄取纳米材料的情况

在超声存在下用细胞计数试剂盒-8（cell counting kit-8

CCK-8）和Annexin

#x02164;-异硫氰酸荧光素（fluorescein isothiocyanate

FITC）试剂评估细胞存活和凋亡的情况。构建人喉癌细胞系Tu686的BALB/c裸小鼠移植瘤模型

通过注射不同类型药物和进行肿瘤区域超声辐照

在治疗期间记录小鼠体重和肿瘤变化

分析比较治疗效果;应用H-E、TUNEL、Ki-67及DHE染色等方法比较肿瘤细胞凋亡和活性氧（reactive oxygen species

ROS）产生情况。

结果：

纳米材料与细胞温育24 h未见明显毒性

仅在超声刺激下导致细胞存活率下降

细胞主要以早期凋亡和晚期凋亡为主。动物实验中仅在第1、3、5和7天经过瘤内注射药物和超声处理

发现仅T-G-C+超声组小鼠肿瘤增长显著被抑制

抑瘤率为100%

且经16 d的治疗

肿瘤区域经超声辐照后可见明显的红色荧光

证实抑瘤作用的核心是ROS的产生。

结论：

生物相容性高的T-G-C纳米颗粒在超声刺激时

激发了SDT和压电治疗的协同作用

通过ROS促进喉癌细胞早期和晚期凋亡

为喉癌提供了一种联合治疗方法

有望促进多学科治疗在耳鼻咽喉科中的应用。

Abstract

Background and purpose:

Laryngeal cancer is a refractory malignant tumor in otolaryngology. Radiotherapy and chemotherapy combined with surgery are often used

however

this treatment method has little improvement on the 5-year survival rate and mortality of patients

and is often accompanied by loss of laryngeal function and drug resistance. This study aimed to explore the effect of sonodynamic therapy (SDT) mediated by nanoacoustic sensitizer T-G-C particles on inhibiting human laryngeal cancer cells

and to provide a new non-invasive treatment for laryngeal cancer.

Methods:

Based on the synthesized T-G-C nanomaterials

the uptake of nanomaterials by Tu686 cells was evaluated under confocal laser scanning microscope

and the cell survival and apoptosis were evaluated by cell counting kit-8 (CCK-8) and Annexin

#x02164;- fluorescein isothiocyanate (FITC) reagent in the presence of ultrasound. BALB/c nude mouse xenograft model was established. The weight and tumor changes of mice were recorded during the treatment by injecting different types of drugs and ultrasonic irradiation in the tumor area

and the treatment effects were analyzed and compared. The apopto

sis and necrosis of tumor cells and the production of reactive oxygen species (ROS) were compared by H-E

TUNEL

Ki-67 and DHE stainings.

Results:

Nanomaterials and cells incubated for 24 hours had no obvious toxicity

only led to the decline of cell survival rate under ultrasonic stimulation

and the cells showed mainly early apoptosis and late apoptosis. In the animal experiment

only after intratumoral injection of drugs and ultrasound treatment on the 1st

3rd

5th and 7th days

the tumor growth of mice in T-G-C+ultrasound group was significantly inhibited

and the tumor inhibition rate is 100%. After 16 d of treatment

obvious red fluorescence could be seen in the tumor area after ultrasound irradiation

which demonstrated that the core of tumor inhibition was the production of ROS.

Conclusion:

Biocompatible T-G-C nanoparticles stimulate the synergy of SDT and piezoelectric therapy when stimulated by ultrasound

and promote the early and late apoptosis of laryngeal cancer cells through ROS

which provides a combined treatment method for laryngeal cancer and promotes the application of multidisciplinary therapy in department of otolaryngology.

关键词

Keywords

references

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