单细胞RNA测序技术揭示卵巢高钙血症型小细胞癌复发病灶的细胞分子特征

康茗贻; 郜意; 胥婧; 康玉; 徐丛剑

doi:10.19401/j.cnki.1007-3639.2022.11.004

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单细胞RNA测序技术揭示卵巢高钙血症型小细胞癌复发病灶的细胞分子特征

专题论著 | 更新时间：2025-12-31

- 单细胞RNA测序技术揭示卵巢高钙血症型小细胞癌复发病灶的细胞分子特征
- Single-cell RNA sequencing reveals tissue architecture in small cell carcinoma of the ovary, hypercalcemic type
- 中国癌症杂志 2022年32卷第11期页码：1065-1073
- 作者机构：
  
  1. 复旦大学附属妇产科医院妇科，上海 200011
  2. 上海市女性生殖内分泌相关疾病重点实验室，上海 200011
  3. 复旦大学上海医学院妇产科学系，上海 200032
- 作者简介：
  
  [ "康茗贻（ORCID: 0000-0003-3469-1754），博士，住院医师。" ]
  康玉（ORCID: 0000-0003-1197-4727），博士，主任医师。
- 基金信息：
  
  上海申康医院发展中心临床科技创新项目(SHDC12020108);上海市老龄化和妇儿健康研究专项(2020YJZX0202)
- DOI：10.19401/j.cnki.1007-3639.2022.11.004
  中图分类号： R737.31
- 收稿：2022-10-20，
  
  修回：2022-11-17，
  
  纸质出版：2022-11-30
- 稿件说明：
移动端阅览
康茗贻, 郜意, 胥婧, 等. 单细胞RNA测序技术揭示卵巢高钙血症型小细胞癌复发病灶的细胞分子特征[J]. 中国癌症杂志, 2022,32(11):1065-1073.

Mingyi KANG, Yi GAO, Jing XU, et al. Single-cell RNA sequencing reveals tissue architecture in small cell carcinoma of the ovary, hypercalcemic type[J]. China Oncology, 2022, 32(11): 1065-1073.
康茗贻, 郜意, 胥婧, 等. 单细胞RNA测序技术揭示卵巢高钙血症型小细胞癌复发病灶的细胞分子特征[J]. 中国癌症杂志, 2022,32(11):1065-1073. DOI： 10.19401/j.cnki.1007-3639.2022.11.004.

Mingyi KANG, Yi GAO, Jing XU, et al. Single-cell RNA sequencing reveals tissue architecture in small cell carcinoma of the ovary, hypercalcemic type[J]. China Oncology, 2022, 32(11): 1065-1073. DOI： 10.19401/j.cnki.1007-3639.2022.11.004.

摘要

背景与目的：

卵巢高钙血症型小细胞癌（small cell carcinoma of the ovary

hypercalcemic type，SCCOHT）是高度恶性的、极为罕见的原发性未分化卵巢癌，本研究旨在分析SCCOHT肿瘤微环境及细胞分子特征，探究潜在药物靶标。

方法：

收集1例SCCOHT患者盆腔复发病灶，应用单细胞RNA测序（single-cell RNA sequencing，scRNA-seq）寻找关键细胞亚群及潜在药物靶点，进一步在细胞学层面进行验证。

结果：

SCCOHT复发病灶中有诱导性多能干细胞（induced pluripotent stem cell，iPSC）、神经施万细胞、神经上皮细胞和巨噬细胞4种细胞亚群和12个细胞亚簇。iPSCs细胞亚群显著上调的基因主要富集于细胞周期调控，其中

PLK

1基因上调最为显著。

PLK

1蛋白在SCCOHT组织切片中呈阳性表达，在SCCOHT细胞系中表达水平升高。

结论：

揭示了SCCOHT的肿瘤微环境由iPSC、神经施万细胞、神经上皮细胞及巨噬细胞组成，鉴定出iPSC关键细胞亚群，寻找到

PLK

1基因作为潜在的治疗靶点。

Abstract

Background and purpose:

Small cell carcinoma of the ovary

hypercalcemic type (SCCOHT) is a highly malignant

extremely rare primary undifferentiated ovarian cancer. This study aimed to reveal the tumor microenvironment and cellular and molecular characteristics of SCCOHT

and to explore potential drug targets.

Methods:

We collected recurrent pelvic lesions from one patient with SCCOHT

and applied single-cell RNA sequencing (scRNA-seq) to explore key cell subsets and potential drug targets. We further verified them at the cytological level.

Results:

There were four cell subsets and twelve cell subclusters of induced pluripotent stem cell (iPSC)

neural Schwann cells

neuroepithelial cells and macrophages in the recurrent lesions of SCCOHT. The expression of genes significantly up-regulated by iPSC cell subgroups were mainly concen

trated in cell cycle regulation

among which

PLK

1 was the most significantly up-regulated gene. PLK1 protein was positively expressed in SCCOHT tissue sections and increased in SCCOHT cell lines.

Conclusion:

It was revealed that the tumor microenvironment of SCCOHT was composed of iPSCs

neural Schwann cells

neuroepithelial cells and macrophages. The key cell subsets of iPSCs were identified

and

PLK

1 gene was found as a potential therapeutic target.

关键词

Keywords

references

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