Fig. 1

Abnormal expression of CPT1A in breast cancer tissue promotes tumor metastasis A, B: The expression of CPT1A mRNA in breast cancer tissues (n=1 085), normal breast tissues (n=291) and breast cancer tissues with different stages was analyzed by GEPIA. C: Using TCGA GEPIA to generate Kaplan-Meier diagram of breast cancer patients. The data of 1 069 breast cancer patients were divided into two groups according to the median percentile of CPT1A expression. D, E: Western blot representation and quantitative analysis of the expression level of CPT1A after overexpression. F, G: Transwell were used to determine the invasive ability of breast cancer cells expressing CPT1A or negative control vector. H, I: Western blot representation and quantitative analysis of the expression level of CPT1A after shRNA knockdown. J, K: Transwell were used to determine the invasive ability of breast cancer cells or negative control cells after CPT1A knock-down. *: P<0.05, compared with normal breast tissues; ***: P<0.001, compared with NC group."

Fig. 2

CPT1A promotes breast cancer cell metastasis by regulating mitochondrial division A, B: Mitotracker living cell mitochondrial imaging and average mitochondrial branch length of cells overexpressing CPT1A or negative control. C, D: Mitotracker living cell mitochondrial imaging and average mitochondrial branch length of shRNA knock-down or negative control cells. E, F: Mitotracker mitochondrial imaging and average mitochondrial branching length of CPT1A overexpressing MDA-MB-231 cells treated with Mdivi-1 and M-1. G, H: Transwell was used to determine the invasive ability of CPT1A cells overexpressed MDA-MB-231 treated with Mdivi-1 and M-1. *: P<0.05, compared with NC group; **: P<0.01, compared with NC group; ***: P<0.001, compared with DMSO group."

Fig. 3

CPT1A activates PGC-1α through ERK1/2 A: Antibody array scanning of MDA-MB-231 cells overexpressing CPT1A. B, C: Western blot analysis and quantitative analysis of ERK1/2-PGC-1α expression in MDA-MB-231 cells over-expressing CPT1A. D, E: Western blot analysis and quantitative analysis of ERK1/2-PGC-1α expression in MDA-MB-231 cells treated with different doses of PD325901 for 8 h. *: P<0.05, compared with 0 nmol/L group; **: P<0.01, compared with NC group or 0 nmol/L group; ***: P<0.001, compared with NC group or 0 nmol/L group."

Fig. 4

CPT1A promotes mitochondrial oxidative phosphorylation A, B: Real-time OCR tracing of MDA-MB-231 cells overexpressing CPT1A and negative control and statistics of respiratory parameters. C, D: Real-time OCR tracing of MDA-MB-231 cells with CPT1A knockdown and negative control and statistics of respiratory parameters. E, F: Western blot analysis of the expression and quantitative analysis of PGC-1α and mitochondrial DNMT1 in MDA-MB-231 cells that overexpressed CPT1A and knocked down CPT1A. G: The expression level of mitochondrial DNA coding gene was measured by Taqman assay, and the data was standardized to 18s rRNA. *: P<0.05, compared with NC group; **: P<0.01, compared with NC group; ***: P<0.001, compared with NC group."