整合素β3在肺腺癌骨转移中的作用及应用价值
穆佳倩, 滕小艳, 魏丽荣, 仇荣, 桂鹏程, 杜玉珍

The role and application value of integrin β3 in bone metastasis of lung adenocarcinoma
MU Jiaqian, TENG Xiaoyan, WEI Lirong, QIU Rong, GUI Pengcheng, DU Yuzhen
图2 A549 ITGB3过表达稳转细胞株的的构建
Fig. 2 Construction of A549 ITGB3 overexpression stable cell line
A: Western blot was performed to detect ITGB3 expression in different lung cancer cell lines; B-C: ITGB3 overexpression level of A549 cells was detected by flow cytometry and Western blot; D-F: Exosomes secreted by lung adenocarcinoma cells were identified by electron microscopy, NTA and Western blot, and the expression level of ITGB3 in exosomes was detected by Western blot.