TMCO1对宫颈癌细胞增殖和迁移能力的影响

陈珣, 郑真霞, 阮雪茹

Effects of TMCO1 on proliferation and migration of cervical cancer cells

CHEN Xun, ZHENG Zhenxia, RUAN Xueru

图2 过表达TMCO1促进宫颈癌HeLa细胞的增殖

Fig. 2 Overexpression of TMCO1 promotes the proliferation of cervical cancer HeLa cells A: A TMCO1 lentiviral expression vector was infected with the cervical cancer cell line HeLa to obtain a cell line stably expressing TMCO1. The cell line infected with the EV was used as a control, and the expression of TMCO1 was confirmed by IB. B: The stably transfected TMCO1 cells and control cells (EV) were subjected to CCK-8 cell proliferation assay. C, D: The stably transfected TMCO1 cells and control cells (EV) were seeded into 6-well plates at 1 000 cells/well, and the number of clones formed was counted after 10 days of culture. E, F: The stably transfected TMCO1 cells and control cells (EV) were labeled with EdU to detect the number of cells undergoing active DNA synthesis. *: P<0.05, **: P<0.01. EV: Empty vector.