TMCO1对宫颈癌细胞增殖和迁移能力的影响

陈珣, 郑真霞, 阮雪茹

Effects of TMCO1 on proliferation and migration of cervical cancer cells

CHEN Xun, ZHENG Zhenxia, RUAN Xueru

图3 敲减TMCO1抑制宫颈癌HeLa细胞的增殖

Fig. 3 Knocking down TMCO1 inhibited the proliferation of cervical cancer HeLa cells A: Two independent shRNAs targeting the TMCO1 gene sequence were designed and cloned into the pLKO.1 lentiviral vector, which was used to infect the cervical cancer cell line HeLa. After puromycin selection, cells with stable knockdown of TMCO1 were obtained, and cells infected with the empty vector (pLKO.1) virus were used as controls. The cells were collected and the knockdown of TMCO1, as well as the phosphorylation of cell cycle inhibitory protein p27 and histone H3 were detected by IB. B, C: The above TMCO1 stable knockdown cells and control cells were seeded into 6-well plates at 1 000 cells/well, and the number of clones formed was counted after 10 days of culture. D, E: The above TMCO1 stable knockdown cells and control cells were EdU labeled to detect the number of cells undergoing active DNA synthesis. *: P<0.05, **: P<0.01, ***: P<0.001, compared with empty vector (pLKO.1).