Fig. 5 TMCO1 regulated cell adhesion and signaling transduction
A: The Flag-TMCO1 cells and control cells (EV) in Figure 2 were collected, cell lysates were prepared and trypsinized, and then analyzed by liquid chromatography-tandem mass spectrometry. Data were collected for principal component analysis, and it can be seen that the two Flag-TMCO1 stable expression cell line samples and the three control cell (EV) samples were clustered together, indicating that the stable expression of TMCO1 caused significant changes in the proteomic level. B: The differentially expressed proteins obtained by quantitative analysis of the protein expression profile data obtained in (A) were analyzed and a volcano plot was drawn, and representative proteins with significant differences were annotated. C, D: GSEA-KEGG pathway enrichment analysis was performed on the differentially expressed proteins in the Flag-TMCO1 stable expression cells and control cells (EV) protein expression groups, showing that pathways related to extracellular matrix-adhesion and PI3K-AKT signaling were significantly upregulated in Flag-TMCO1 stable expression cells, while ribosome-related pathways were downregulated.
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