关键词: 单细胞测序, 实体瘤异质性, 转移, 耐药, 免疫

Abstract: Heterogeneity is one of the most important characteristics of solid tumors. Therefore, it is essential to identify different cell subsets and related gene expression levels in solid tumor and its microenvironment. Single-cell sequencing uses DNA second-generation sequencing technology to analyze the DNA, RNA or DNA methylation level of a single cell, reveal its genome, transcriptome and epigenetic characteristics, and understand the state and function of a single cell. Single-cell sequencing can not only further identify the heterogeneity of cells in solid tumors, but also help clarify the molecular mechanisms of tumor development, metastasis, drug resistance and immune escape, making clinical diagnosis, treatment and prognosis of solid tumors more accurate. This article briefly summarized the advances of single-cell sequencing, and reviewed the application and progress of single-cell sequencing in solid tumor research.

Key words: Single-cell sequencing, Heterogeneity of solid tumors, Metastasis, Drug resistance, Immunity