关键词: 慢性粒细胞白血病, 3-磷酸肌醇依赖性激酶, 凋亡信号调节激酶1, 细胞凋亡

Abstract: Background and purpose: 3-phosphoinositide-dependent kinase-1 (PDK1) plays vital role in cell growth, proliferation and survival. Dysfunctional PDK1 involves in various carcinogenesis. This study aimed to determine the abundance of PDK1 in chronic myelogenous leukemia (CML) cells and study the molecular basis of its contribution to anti-apoptotic phenotype. Methods: The expression of protein was detected by Western blot. Flow cytometry was conducted to analyze the apoptotic cells. Clone formation assay and cell counting kit-8 (CCK-8) were employed to examine the inhibition of proliferation. Results: In comparison with heathy donors, PDK1 in CML cells was significantly over-expressed (P<0.05). In K562 and KU812 cell lines, blockage of PDK1 resulted in remarkable reduction of growth and induction of apoptosis (P<0.01), which was further validated by cleavage of the caspase-3 and its substrate poly (ADP-ribose) polymerase (PARP). The PDK1 inhibitor, GSK2334470 lowered the phosphorylation, but not the total PDK1 level (in K562 cells, P<0.05; in KU812 cells, P<0.01). Besides, the decrease in protein kinase B (AKT) and downstream glycogen synthase kinase-3β (GSK-3β) phosphorylation was also found in two cell lines. However, no significant change of β-catenin was detected, consequently, no effect was found on the expression of c-Myc, the targeted gene of this pathway (P>0.05). Moreover, PDK1 inhibition caused evident decrease in apoptosis signal-regulating kinase 1 (ASK1) phosphorylation (P<0.001), which further led to activation of c-Jun N-terminal kinase (JNK) and Bcl-2 interacting mediator of cell death (Bim). Finally, GSK2334470 enhanced the sensitivity of imatinib (P<0.01). Conclusion: PDK1 inhibition triggered cell death by modulation of ASK1/JNK/Bim cascade.

Key words: Chronic myelogenous leukemia, 3-phosphoinositide-dependent kinase-1, Apoptosis signal-regulating kinase 1, Apoptosis