膜辅助蛋白CD46 rs1970530遗传变异对肝癌发病风险的影响

doi:10.19401/j.cnki.1007-3639.2020.09.003

中国癌症杂志 ›› 2020, Vol. 30 ›› Issue (9): 656-660.doi: 10.19401/j.cnki.1007-3639.2020.09.003

膜辅助蛋白CD46 rs1970530遗传变异对肝癌发病风险的影响

徐珊珊 ¹ ，宋琴琴 ¹ ，仵红娇 ²，付　宁 ² ，车玲玉 ² ，金　敏 ¹，刘　冲 ¹，韩素桂 ³ ，张雪梅 ² ，张　志¹

1. 华北理工大学附属唐山市工人医院肿瘤科，河北唐山 063000 ；
2. 华北理工大学生命科学学院，河北唐山 063210 ；
3. 华北理工大学附属唐山市人民医院核医学检验科，河北唐山 063000

出版日期:2020-09-30 发布日期:2020-10-10
通信作者: 张　志　E-mail: zhi1969@163.com
基金资助:
国家自然科学基金（81101483）；河北省自然科学基金重点项目（H2017209233）；唐山市科技创新团队培养计划（14130225B）。

Genetic variation in membrane cofactor protein CD46 rs1970530 affects the risk of hepatocellular carcinoma

XU Shanshan¹ , SONG Qinqin¹ , WU Hongjiao ², FU Ning² , CHE Lingyu ², JIN Min¹ , LIU Chong¹ , HAN Sugui ³ , ZHANG Xuemei ² , ZHANG Zhi ¹

1. Department of Oncology, Tangshan Gongren Hospital, North China University of Science and Technology, Tangshan 063000, Hebei Province, China; 2. College of Life Sciences, North China University of Science and Technology, Tangshan 063210, Hebei Province, China; 3. Nuclear Medicine Laboratory, Tangshan People’s Hospital, North China University of Science and Technology, Tangshan 063000, Hebei Province, China

Published:2020-09-30 Online:2020-10-10
Contact: ZHANG Zhi E-mail: zhi1969@163.com
Supported by:

摘要/Abstract

摘要： 背景与目的：膜辅助蛋白CD46可保护宿主细胞免受补体依赖的细胞毒性作用，研究表明，CD46可能作为肝细胞癌（hepatocellular carcinoma，HCC）患者的潜在生物标志物。探讨CD46基因表达及启动子区遗传变异与HCC发病风险的关系。方法：通过基因表达谱交互分析（gene expression profiling interactive analysis，GEPIA）在线网站分析HCC组织和正常肝组织CD46表达的差异；2011—2015年在华北理工大学附属唐山市工人医院和华北理工大学附属唐山市人民医院经病理学检查确诊且未经放化疗的240例HCC患者作为病例组，对照组为同时期入院体检的500名健康人群。采用聚合酶链反应-限制性片段长度多态性分析（polymerase chain reaction-restriction fragment length polymorphism，PCR-RFLP）法进行基因分型，检测两组基因型频率和等位基因频率，评估CD46 rs1970530遗传变异与HCC发病风险的关系。结果：CD46在HCC组织与正常组织中的表达差异有统计学意义（P<0.05）。经非条件logistic回归分析发现，CD46 rs1970530至少携带一个G等位基因型在病例组及对照组之间差异有统计学意义（OR=0.666，95% CI：0.448~0.990，P<0.05）；两组间等位基因G频率差异有统计学意义（OR=0.689，95% CI：0.478~0.994，P<0.05）。分层分析结果显示，至少携带一个G等位基因者可降低高年龄组（>60岁）（P=0.048）和男性（P=0.023）人群的HCC发病风险，在女性和低年龄组（≤60岁）中差异无统计学意义（P>0.05）。按吸烟状态进行分层分析，rs1970530变异与HCC易感性无明显关联（P>0.05）。结论：HCC中CD46基因高表达及CD46 rs1970530遗传变异影响HCC发病风险。

关键词: 膜辅助蛋白CD46, 肝细胞癌, 单核苷酸多态性, 易感性

Abstract: Background and purpose: Membrane cofactor protein CD46 protects host cells from complement-dependent cytotoxicity and may be a potential molecular marker for hepatocellular carcinoma (HCC). This study aimed to investigate the correlation between the gene expression of CD46 with the genetic variation of CD46 promoter and the risk of HCC. Methods: The difference in CD46 expression between HCC tissues and normal liver tissues was analyzed by gene expression profiling interactive analysis (GEPIA) online website. From 2011 to 2015, 240 HCC patients were confirmed by pathological diagnosis in Tangshan Gongren Hospital, North China University of Science and Technology and Tangshan People’s Hospital, North China University of Science and Technology, and 500 healthy people enrolled as the control group in the same period. Genotyping was performed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to detect genotype frequencies and allele frequencies between two groups. The relationship between the genetic variation of CD46 rs1970530 and the risk of HCC was evaluated. Results: The expression of CD46 was significantly different between HCC tissues and normal tissues (P<0.05). Unconditional logistic regression analysis indicated the difference in CD46 rs1970530 genotype carrying at least one G allele was statistically significant between the case group and the control group (OR=0.666, 95% CI: 0.448-0.990, P<0.05), and there was also a significant difference in the frequency of the allele G (OR=0.689, 95% CI: 0.478-0.994, P<0.05). The stratification analysis showed that CD46 rs1970530 genotype carrying at least one G allele could reduce the risk of HCC in the advanced age group (>60 years) (P=0.048) and men (P=0.023), however, there was no difference in women and younger age groups between the HCC and the healthy control (≤60 years) (P>0.05). Smoking stratification analysis indicated that there was no significant correlation between rs1970530 mutation and HCC susceptibility (P>0.05). Conclusion: The high gene expression of CD46 in HCC and the genetic variation of CD46 rs1970530 affect the risk of HCC.

Key words: Membrane cofactor protein CD46, Hepatocellular carcinoma, Single nucleotide polymorphism, Susceptibility

徐珊珊 , 宋琴琴 , 仵红娇 , 付　宁 , 车玲玉 , 金　敏 , 刘　冲 , 韩素桂 , 张雪梅 , 张　志 . 膜辅助蛋白CD46 rs1970530遗传变异对肝癌发病风险的影响[J]. 中国癌症杂志, 2020, 30(9): 656-660.

XU Shanshan , SONG Qinqin , WU Hongjiao , FU Ning , CHE Lingyu , JIN Min , LIU Chong , HAN Sugui , ZHANG Xuemei , ZHANG Zhi . Genetic variation in membrane cofactor protein CD46 rs1970530 affects the risk of hepatocellular carcinoma[J]. China Oncology, 2020, 30(9): 656-660.

[1]	杨　佳, 刘舒媛, 王莹莹, 李盈甫, 马千里, 李　玙, 王永蓉, 李传印, 谭　芳 . 云南省汉族人群TNF-α基因多态性与非小细胞肺癌发生、发展的相关性[J]. 中国癌症杂志, 2021, 31(7): 616-628.
[2]	肖　锋, 肖静文, 邵建国, 陈丽燕, 顾春燕 . PD-1在肝细胞癌肿瘤浸润淋巴细胞中的表达及与预后的相关性[J]. 中国癌症杂志, 2021, 31(5): 419-427.
[3]	牛　娜, 林岩松 . ⁹⁰ Y微球选择性内放射治疗在肝细胞癌中的应用及研究进展[J]. 中国癌症杂志, 2021, 31(5): 428-434.
[4]	刘　敏, 陈添亮, 郭　娟, 伊雪, 高洪泉, 巫佳翠, 王玉孝 . 蛋白激酶CβⅡ通过IL-6自分泌途径促进肝细胞癌侵袭的研究[J]. 中国癌症杂志, 2021, 31(4): 268-276.
[5]	章　俊, 董宇华, 杨　叶, 张梦琪, 何　常 . Regorafenib联合TAS102新策略治疗肝细胞癌的研究[J]. 中国癌症杂志, 2021, 31(4): 294-301.
[6]	张　欣, 周　成, 周恺乾, 柏乾明, 蒋冬先, 宿杰阿克苏, 侯英勇, 王　征, 纪　元 . 肉瘤样肝细胞癌中MET基因扩增与预后的相关性分析[J]. 中国癌症杂志, 2020, 30(9): 641-649.
[7]	皮思蝶, 郑小川, 毛彬力, 崔　静, 胡　源. SAMHD1通过调控p27表达抑制肝细胞癌细胞增殖的研究[J]. 中国癌症杂志, 2020, 30(7): 489-496.
[8]	蒙秋华, 田　婧, 覃　妍, 蒙秋晓, 董　敏. 蛋白酪氨酸磷酸酶受体D通过STAT3途径影响肝癌细胞PD-L1表达的机制研究[J]. 中国癌症杂志, 2020, 30(4): 261-267.
[9]	虞　梅,梅　琪,韩莹莹,翟凌云,沙红芳,徐祥勇,倪　娟,崔建巍,丁　红 . 超声造影灌注时相分析在监控肝硬化结节演变和恶变中的临床价值[J]. 中国癌症杂志, 2019, 29(9): 709-714.
[10]	马家强，曹春梅，Shyamal Goswami,陈　沁，高　强，张晓明. 肝细胞癌组织ICOS+T细胞的多重免疫组织化学检测及其预后价值研究[J]. 中国癌症杂志, 2019, 29(5): 352-361.
[11]	赵雨笛，谭芳，严志凌，李盈甫，戴书颖，周紫云，张新文，刘舒媛，史荔，姚宇峰. CD44基因多态性与宫颈癌及非小细胞肺癌的相关性[J]. 中国癌症杂志, 2019, 29(4): 277-283.
[12]	彭雪强,杨　良,张小东,等. 自噬相关基因5在肝癌组织中的表达及临床意义[J]. 中国癌症杂志, 2018, 28(5): 321-326.
[13]	武明辉,谭红娜,吴青霞,等. 肝脏磁共振T2WI图像纹理特征预测肝细胞癌患者微血管侵犯的价值[J]. 中国癌症杂志, 2018, 28(3): 191-196.
[14]	肖金成,康鑫鑫,白淇文,等. 经肝动脉化疗栓塞联合射频消融治疗原发性肝细胞癌合并门静脉癌栓的预后影响因素分析[J]. 中国癌症杂志, 2018, 28(3): 222-228.
[15]	王秀月,赵　川,陈　彻,等. 长链非编码RNA作为肝细胞癌诊断新型血清标志物的meta分析[J]. 中国癌症杂志, 2018, 28(3): 229-235.

膜辅助蛋白CD46 rs1970530遗传变异对肝癌发病风险的影响

Genetic variation in membrane cofactor protein CD46 rs1970530 affects the risk of hepatocellular carcinoma

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价