中国癌症杂志 ›› 2021, Vol. 31 ›› Issue (11): 1110-1114.doi: 10.19401/j.cnki.1007-3639.2021.11.010

• 综述 • 上一篇    下一篇

单细胞转录组测序及其在乳腺癌中的应用

韩香臣,李小光,胡 欣   

  1. 复旦大学附属肿瘤医院乳腺外科,复旦大学上海医学院肿瘤学系,上海 200032
  • 出版日期:2021-11-30 发布日期:2021-12-03
  • 通信作者: 胡 欣 E-mail: xinhu@fudan.edu.cn
  • 基金资助:
    国家自然科学基金(81803555)。

Single-cell RNA sequencing and its application in breast cancer

HAN Xiangchen, LI Xiaoguang, HU Xin   

  1. Department of Breast Surgery, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China
  • Published:2021-11-30 Online:2021-12-03
  • Contact: HU Xin E-mail: xinhu@fudan.edu.cn

摘要: 乳腺癌占全部女性癌症的17.1%,位于中国女性癌症发病率首位。尽管目前根据乳腺癌分子分型采用的个体化治疗策略使乳腺癌治疗取得了阶段性突破,但是肿瘤异质性、耐药、转移等问题依然阻碍我们进一步提升临床疗效。单细胞转录组测序是一项能在单细胞水平上进行无偏倚、高通量、高分辨率全转录组学分析的新技术。该技术能以单细胞分辨率分析肿瘤内部基因表达特征,对认识乳腺癌具有不可替代的作用。就单细胞测序技术在肿瘤异质性、肿瘤微环境、转移、耐药等方面的应用进行综述,以期更加深刻地了解该技术并为乳腺癌研究提供全新思路。

关键词: 单细胞转录组测序, 乳腺癌, 肿瘤异质性, 肿瘤微环境

Abstract: Breast cancer, accounting for 17.1% of the female cancers, is the most frequently diagnosed malignancy among women in China. Current treatment strategies based on the breast cancer subtype have remarkably improved the prognosis, however, obstacles remain. It’s of great importance to elucidate problems, including the tumor heterogeneity, metastases and therapeutic resistance, to promote therapeutic efficacy. Single-cell RNA sequencing is a novel method that enables unbiased, high-throughput and high-resolution transcriptomic analysis at the single-cell level. It could profile intratumor gene expression features at single-cell resolution and improve our understanding of breast cancer. This review illustrated the application of single-cell RNA sequencing in breast cancer from diverse aspects to obtain further knowledge of this emerging technology and provided new insights into breast cancer research.

Key words: Single-cell RNA sequencing, Breast cancer, Tumor heterogeneity, Tumor microenvironment