关键词: 肺鳞癌, 肌动蛋白相关蛋白2/3复合体5, 增殖, 迁移, 侵袭

Abstract:

Background and purpose: Actin related protein 2/3 complex subunit 5 (ARPC5) is involved in the packaging of actin, and then affects the mobility of cells. It plays an important role in the formation of invadopodia of cancer cells. This study aimed to investigate the effect of ARPC5 gene-silence on cell proliferation, migration and invasion of lung squamous carcinoma cell line SK-MES-1. Methods: Under the induction of LipofectamineTM RNAiMAX, the recombinant of si-ARPC5 was transfected into SK-MES-1. The experiment set up 3 groups: blank (mock) group, negative control (NC) group and si-ARPC5 transfected group. Cell proliferation, migration and invasion assays were investigated by cell counting kit(CCK-8) method, scratch assay and Transwell Chambers respectively. Results: The result of qRT-PCR and Western blot displayed downregulation of ARPC5 in mRNA and protein level of SK-MES-1 cell. Proliferation rate of SK-MES-1 cells in 72 h after si-ARPC5 transfection was lower than those in mock and NC groups (t=7.993, t=8.681, P<0.05). Compared to the mock and NC groups, the scratch repairing rate of the si-ARPC5 group was lower [(43.32±0.23)% vs (73.11±0.43)% and (76.58±0.88)%] (t=7.348, t=10.614, P<0.05); And the number of penetrating the membrane was significantly reduced [(27±6) vs (101±11) and (92±9)] (t=10.229, t=8.391, P<0.05). Conclusion: Downregulation of ARPC5 gene can inhibit the proliferation, migration, and invasion of SKMES-1 cell.

Key words: Lung squamous cell carcinoma, Actin related protein 2/3 complex subunit 5, Proliferation, Migration, Invasion