中国癌症杂志 ›› 2019, Vol. 29 ›› Issue (7): 535-539.doi: 10.19401/j.cnki.1007-3639.2019.07.009

• 综述 • 上一篇    下一篇

单细胞测序技术在实体瘤研究中的应用进展

郑小翠  综述 ,汪希鹏  审校   

  1. 上海交通大学医学院附属新华医院妇产科,上海 200092
  • 出版日期:2019-07-30 发布日期:2019-07-12
  • 通信作者: 汪希鹏 E-mail: wangxipeng@xinhuamed.com.cn
  • 基金资助:
    国家自然科学基金(81874103)。

Progress of single-cell sequencing in solid tumors

ZHENG Xiaocui, WANG Xipeng   

  1. Department of Obstetrics and Gynecology, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
  • Published:2019-07-30 Online:2019-07-12
  • Contact: WANG Xipeng E-mail: wangxipeng@xinhuamed.com.cn

摘要: 肿瘤异质性是实体瘤组织的重要特征之一,其多样性和复杂性给实体瘤的基础研究和临床诊治带来了极大的困难。因此,鉴别实体瘤内及其微环境中不同的细胞亚群及与之相关的基因表达水平就显得十分重要。单细胞测序技术是利用DNA二代测序技术对单个细胞的DNA、RNA或DNA甲基化水平进行分析,分别揭示其基因组、转录组和表观遗传学特征,了解单个细胞功能和所处的状态。单细胞测序分析不仅能进一步鉴定实体瘤内细胞的异质性,还有助于阐明肿瘤发生、发展、转移、耐药和免疫逃逸等分子作用机制,从而使实体瘤的临床诊治和转归预测变得更加准确。简要总结目前单细胞测序技术的发展,对单细胞测序在实体瘤研究中的应用与进展进行综述。

关键词: 单细胞测序, 实体瘤异质性, 转移, 耐药, 免疫

Abstract: Heterogeneity is one of the most important characteristics of solid tumors. Therefore, it is essential to identify different cell subsets and related gene expression levels in solid tumor and its microenvironment. Single-cell sequencing uses DNA second-generation sequencing technology to analyze the DNA, RNA or DNA methylation level of a single cell, reveal its genome, transcriptome and epigenetic characteristics, and understand the state and function of a single cell. Single-cell sequencing can not only further identify the heterogeneity of cells in solid tumors, but also help clarify the molecular mechanisms of tumor development, metastasis, drug resistance and immune escape, making clinical diagnosis, treatment and prognosis of solid tumors more accurate. This article briefly summarized the advances of single-cell sequencing, and reviewed the application and progress of single-cell sequencing in solid tumor research.

Key words: Single-cell sequencing, Heterogeneity of solid tumors, Metastasis, Drug resistance, Immunity