• 论著 •

### 白细胞介素35和白细胞介素37与结直肠癌临床病理学特征及预后的相关性研究

1. 1. 北华大学医学院病理教研室，吉林 吉林 132000 ；
2. 上海交通大学医学院附属同仁医院病理科，上海 200336 ；
3. 上海市长宁区疾病预防控制中心，上海 200050
• 出版日期:2020-06-30 发布日期:2020-07-16
• 通信作者: 董志恒　E-mail: 2754855687@qq.com
• 基金资助:
上海市自然科学基金（16ZR1432600）。

### Correlation between interleukin 35 or interleukin 37 and clinicopathology and prognosis of colorectal cancer

CHEN Feier 1,2 , ZHANG Feng 2 , TAN Zhenyu 2 , XIA Qinghua 3 , BAO Shisan 2 , TAO Kun 2 , DONG Zhiheng 1

1. 1. Discipline of Pathology, Beihua University School of Medicine, Jilin 132000, Jilin Province, China; 2. Department of Pathology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, China; 3. Center for Disease Control and Prevention of Changning District, Shanghai 200050, China
• Published:2020-06-30 Online:2020-07-16
• Contact: DONG Zhiheng E-mail: 2754855687@qq.com

Abstract: Background and purpose: Colorectal cancer (CRC) is the third most common cancer in the world. Recently, it is suggested that interleukin (IL)-35 and/or IL-37 are associated with CRC, but the precise underlying mechanism has not been fully explored. The aim of this study was to explore the correlation between IL-35 or IL-37 and clinicopathology of CRC, and to determine whether IL-35 or IL-37 could be used as prognosis biomarker for CRC patients. Methods: Tissue array was generated from both CRC (n=191) and the matched non-cancer tissues (n=141) from the same patient’s adjacent tissues from 2013 to 2017 in Department of Pathology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine. Quantitative analysis for IL-35 and IL-37 was performed on these immunohistochemically (IHC) labelled tissues using Image-Pro Plus software, for comparison between cancer and non-cancer tissues. We investigated the correlation between IL-35/IL-37 in the CRC tissues and pathology, as well as prognosis. Results: The expression of IL-35 was decreased by 50% in CRC tissues compared with non-cancer tissues (P<0.000 1). In contrast, the expression of IL-37 was increased by 40% in CRC tissues compared with non-cancer tissues (P=0.012). Furthermore, the expression of IL-35, but not IL-37, in cancer tissues (HR=0.39, 95% CI: 0.16-0.97, P=0.04) was an independent predictor of postoperative survival in CRC patients, using the multivariate survival analysis. Conclusion: The expression of IL-35 or IL-37 may be involved in the development of CRC. The expression of IL-35 is believed to be an independent predictor of postoperative survival in CRC patients. The precise mechanism of IL-35 and IL-37 in the development of CRC in vivo will be determined in future.