中国癌症杂志 ›› 2020, Vol. 30 ›› Issue (6): 456-461.doi: 10.19401/j.cnki.1007-3639.2020.06.008

• 论著 • 上一篇    下一篇

白细胞介素35和白细胞介素37与结直肠癌临床病理学特征及预后的相关性研究

陈飞儿 1,2 ,张 凤 2 ,谈振宇 2 ,夏庆华 3 ,包士三 2 ,陶 琨 2 ,董志恒 1   

  1. 1. 北华大学医学院病理教研室,吉林 吉林 132000 ;
    2. 上海交通大学医学院附属同仁医院病理科,上海 200336 ;
    3. 上海市长宁区疾病预防控制中心,上海 200050
  • 出版日期:2020-06-30 发布日期:2020-07-16
  • 通信作者: 董志恒 E-mail: 2754855687@qq.com
  • 基金资助:
    上海市自然科学基金(16ZR1432600)。

Correlation between interleukin 35 or interleukin 37 and clinicopathology and prognosis of colorectal cancer

CHEN Feier 1,2 , ZHANG Feng 2 , TAN Zhenyu 2 , XIA Qinghua 3 , BAO Shisan 2 , TAO Kun 2 , DONG Zhiheng 1   

  1. 1. Discipline of Pathology, Beihua University School of Medicine, Jilin 132000, Jilin Province, China; 2. Department of Pathology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, China; 3. Center for Disease Control and Prevention of Changning District, Shanghai 200050, China
  • Published:2020-06-30 Online:2020-07-16
  • Contact: DONG Zhiheng E-mail: 2754855687@qq.com

摘要: 背景与目的:结直肠癌(colorectal cancer,CRC)为世界第三常见恶性肿瘤,近年来研究者认为白细胞介素(interleukin,IL)-35和(或)IL-37与CRC的发展有关,但其作用机制尚未阐明。探究IL-35和IL-37在CRC发展中的作用及其可能的机制,分析IL-35和IL-37与CRC患者预后的相关性。方法:收集2013年—2017年在上海交通大学医学院附属同仁医院接受治疗的191例CRC患者手术病理蜡块的肿瘤组织,与其匹配的非癌组织是来源于同一患者的肠癌手术切缘蜡块组织。应用免疫组织化学(immunohistochemistry,IHC)染色法将CRC患者的癌组织与非癌组织染色,并运用Image-Pro Plus将IHC染色阳性部分定量分析,结合随访结果,探讨癌组织与非癌组织中IL-35和IL-37的表达水平与CRC临床病理学特征及预后的相关性。结果:与非癌组织相比,CRC组织中IL-35的表达量减少了50%(P<0.000 1)。CRC组织中IL-37的表达量与非癌组织相比增加了40%(P=0.012)。多因素生存分析显示,癌组织中IL-35(HR=0.39;95% CI:0.16~0.97;P=0.04)的表达水平是CRC患者术后生存的独立预测指标。结论:IL-35和IL-37蛋白的表达水平可能与CRC的发展有关,IL-35的表达水平可能是CRC患者术后生存的独立预测指标。

关键词: 结直肠癌, 白细胞介素35, 白细胞介素37, 预后, 多因素生存分析

Abstract: Background and purpose: Colorectal cancer (CRC) is the third most common cancer in the world. Recently, it is suggested that interleukin (IL)-35 and/or IL-37 are associated with CRC, but the precise underlying mechanism has not been fully explored. The aim of this study was to explore the correlation between IL-35 or IL-37 and clinicopathology of CRC, and to determine whether IL-35 or IL-37 could be used as prognosis biomarker for CRC patients. Methods: Tissue array was generated from both CRC (n=191) and the matched non-cancer tissues (n=141) from the same patient’s adjacent tissues from 2013 to 2017 in Department of Pathology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine. Quantitative analysis for IL-35 and IL-37 was performed on these immunohistochemically (IHC) labelled tissues using Image-Pro Plus software, for comparison between cancer and non-cancer tissues. We investigated the correlation between IL-35/IL-37 in the CRC tissues and pathology, as well as prognosis. Results: The expression of IL-35 was decreased by 50% in CRC tissues compared with non-cancer tissues (P<0.000 1). In contrast, the expression of IL-37 was increased by 40% in CRC tissues compared with non-cancer tissues (P=0.012). Furthermore, the expression of IL-35, but not IL-37, in cancer tissues (HR=0.39, 95% CI: 0.16-0.97, P=0.04) was an independent predictor of postoperative survival in CRC patients, using the multivariate survival analysis. Conclusion: The expression of IL-35 or IL-37 may be involved in the development of CRC. The expression of IL-35 is believed to be an independent predictor of postoperative survival in CRC patients. The precise mechanism of IL-35 and IL-37 in the development of CRC in vivo will be determined in future.

Key words: Colorectal Cancer, Interleukin-35, Interleukin-37, Prognosis, Multivariate analysis