• 论著 •

### 膜辅助蛋白CD46 rs1970530遗传变异对肝癌发病风险的影响

1. 1. 华北理工大学附属唐山市工人医院肿瘤科，河北 唐山 063000 ；
2. 华北理工大学生命科学学院，河北 唐山 063210 ；
3. 华北理工大学附属唐山市人民医院核医学检验科，河北 唐山 063000
• 出版日期:2020-09-30 发布日期:2020-10-10
• 通信作者: 张　志　E-mail: zhi1969@163.com
• 基金资助:
国家自然科学基金（81101483）；河北省自然科学基金重点项目（H2017209233）；唐山市科技创新团队培养计划（14130225B）。

### Genetic variation in membrane cofactor protein CD46 rs1970530 affects the risk of hepatocellular carcinoma

XU Shanshan 1 , SONG Qinqin 1 , WU Hongjiao 2 , FU Ning 2 , CHE Lingyu 2 , JIN Min 1 , LIU Chong 1 , HAN Sugui 3 , ZHANG Xuemei 2 , ZHANG Zhi 1

1. 1. Department of Oncology, Tangshan Gongren Hospital, North China University of Science and Technology, Tangshan 063000, Hebei Province, China; 2. College of Life Sciences, North China University of Science and Technology, Tangshan 063210, Hebei Province, China; 3. Nuclear Medicine Laboratory, Tangshan People’s Hospital, North China University of Science and Technology, Tangshan 063000, Hebei Province, China
• Published:2020-09-30 Online:2020-10-10
• Contact: ZHANG Zhi E-mail: zhi1969@163.com
• Supported by:

Abstract: Background and purpose: Membrane cofactor protein CD46 protects host cells from complement-dependent cytotoxicity and may be a potential molecular marker for hepatocellular carcinoma (HCC). This study aimed to investigate the correlation between the gene expression of CD46 with the genetic variation of CD46 promoter and the risk of HCC. Methods: The difference in CD46 expression between HCC tissues and normal liver tissues was analyzed by gene expression profiling interactive analysis (GEPIA) online website. From 2011 to 2015, 240 HCC patients were confirmed by pathological diagnosis in Tangshan Gongren Hospital, North China University of Science and Technology and Tangshan People’s Hospital, North China University of Science and Technology, and 500 healthy people enrolled as the control group in the same period. Genotyping was performed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to detect genotype frequencies and allele frequencies between two groups. The relationship between the genetic variation of CD46 rs1970530 and the risk of HCC was evaluated. Results: The expression of CD46 was significantly different between HCC tissues and normal tissues (P<0.05). Unconditional logistic regression analysis indicated the difference in CD46 rs1970530 genotype carrying at least one G allele was statistically significant between the case group and the control group (OR=0.666, 95% CI: 0.448-0.990, P<0.05), and there was also a significant difference in the frequency of the allele G (OR=0.689, 95% CI: 0.478-0.994, P<0.05). The stratification analysis showed that CD46 rs1970530 genotype carrying at least one G allele could reduce the risk of HCC in the advanced age group (>60 years) (P=0.048) and men (P=0.023), however, there was no difference in women and younger age groups between the HCC and the healthy control (≤60 years) (P>0.05). Smoking stratification analysis indicated that there was no significant correlation between rs1970530 mutation and HCC susceptibility (P>0.05). Conclusion: The high gene expression of CD46 in HCC and the genetic variation of CD46 rs1970530 affect the risk of HCC.