中国癌症杂志 ›› 2016, Vol. 26 ›› Issue (12): 974-980.doi: 10.19401/j.cnki.1007-3639.2016.12.003

• 论著 • 上一篇    下一篇

降调ACSS2对非小细胞肺癌A549细胞增殖、凋亡和迁移的影响

卢晓霞1,2,常 淑3,毕明宏1,王雅萍1   

  1. 1. 蚌埠医学院第一附属医院肿瘤内科,安徽 蚌埠 233000 ;
    2. 蚌埠医学院组织移植安徽省重点实验室,安徽 蚌埠 233000 ;
    3. 蚌埠医学院第一附属医院图书馆,安徽 蚌埠 233000
  • 出版日期:2016-12-30 发布日期:2017-01-23
  • 通信作者: 毕明宏 E-mail: bmh2003@126.com

The influence of ACSS2 knockdown on the proliferation, apoptosis and migration of NSCLC cell line A549

LU Xiaoxia1,2, CHANG Shu3, BI Minghong1, WANG Yaping1   

  1. 1. Department of Medical Oncology, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, Anhui Province, China; 2. Anhui Key Laboratory of Tissue Transplantation of Bengbu Medical College, Bengbu 233000, Anhui Province, China; 3. Department of Library, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, Anhui Province, China
  • Published:2016-12-30 Online:2017-01-23
  • Contact: BI Minghong E-mail: bmh2003@126.com

摘要: 背景与目的:代谢水平的改变是肿瘤细胞生长的主要特征之一,有研究证实,乙酰辅酶A合成酶2(cytosolic acetyl-CoA synthetase 2,ACSS2)在肿瘤细胞的代谢中起着至关重要的作用。本研究拟通过RNA干扰技术抑制非小细胞肺癌(non-small cell lung cancer,NSCLC)A549细胞中ACSS2的表达,探讨ACSS2对A549细胞增殖、凋亡和迁移的影响。方法:设计并合成针对ACSS2的特异性干扰片段ACSS2-siRNA及与ACSS2没有同源性的阴性对照,瞬时转染NSCLC A549细胞,通过实时荧光定量聚合酶链反应(real-time fluorescent quantitative polymerase chain reaction,RTFQ-PCR)检测ACSS2 mRNA的表达情况,四甲基偶氮唑盐(methyl thiazolyl tetrazolium,MTT)实验检测转染组与对照组细胞的增殖情况,流式细胞仪检测细胞的凋亡率,细胞划痕实验检测细胞迁移能力。结果:通过转染体外合成的干扰片段ACSS2-siRNA,NSCLC A549细胞中ACSS2 mRNA呈显著低表达。ACSS2-siRNA干扰组的细胞较对照组增殖活性明显减弱,凋亡增加,迁移能力减弱。结论:降调ACSS2表达能显著抑制A549细胞增殖、迁移能力,促进凋亡尤其是早期凋亡明显增加。

关键词: 肺肿瘤, 乙酰辅酶A合成酶2, RNA干扰

Abstract: Background and purpose: Metabolism change is one of the main characteristics of the tumor development. Many studies have confirmed that cytosolic acetyl-CoA synthetase 2 (ACSS2) plays a critical role in hydrocarbon metabolism of cancer cells. This study aimed to explore the effect of ACSS2 on cellular proliferation, apoptosis and migration of A549 cells by RNA interference. Methods: The ACSS2 interference fragment ACSS2-siRNA and negative control were designed and synthesized for RNA interference followed by the transient transfection in non-small cell lung cancer (NSCLC) cell line A549. Real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR) was used to detect ACSS2 mRNA expression. Methyl thiazolyl tetrazolium (MTT), flow cytometry and wound healing assay were used to detect cell proliferation, apoptosis rate and migration. Results: The expression of ACSS2 mRNA was significantly decreased after transfection with the interference fragment ACSS2-siRNA in NSCLC cell line A549. The proliferation and migration activity of ACSS2-siRNA treated cells were decreased significantly compared with the control group. The apoptosis rate, especially the early apoptosis, was increased.. Conclusion: Knockdown of the ACSS2 expression in NSCLC cell line A549 can significantly inhibit the cell proliferation, migration ability and pro- mote the apoptosis rate, especially early apoptosis. This study indicates that ACSS2 may contribute to the progression of human lung adenocarcinoma and may have the potential to serve as a novel therapeutic target.

Key words: Lung neoplasm, Cytosolic acetyl-CoA synthetase 2, RNA interference