最新刊期
卷 27 ， 期 10 ， 2017
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- 摘要：Background and purpose: The expression of Fibulin-5, a tumor suppressor, is reduced in lung cancer tissues. High mobility group box 1 (HMGB1), overexpressed in lung cancer, can promote the invasion and metastasis of the tumor. This study aimed to investigate the molecular mechanism of Fibulin-5 inhibiting the proliferation and metastasis of lung cancer cells. Method: Firstly the expressions of Fibulin-5 and HMGB1 in lung epithelial cells and lung cancer cells were detected. Then transfection reagents were used to transfect Fibulin-5 plasmid and HMGB1 siRNA into A549 cells, which achieved Fibulin-5 overexpression and HMGB1 down-regulation. MTT was used to detect the cell proliferation. Transwell was used to examine the ability of cell invasion and migration. HMGB1 mRNA expression was detected by real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR). The secretion of HMGB1 protein was analyzed by enzyme-linked immunosorbent assay (ELISA). Western blot was used to detect the levels of HMGB1, cyclin D1, matrix metalloproteinases (MMPs), and TLR4/NF-κB pathway related proteins. Results: In A549 cell, Fibulin-5 expression was down-regulated and HMGB1 expression was up-regulated. After overexpression of Fibulin-5 or knockdown of HMGB1 in A549 cells, the levels of HMGB1, cyclin D1, MMP2, MMP7 and MMP9 were significantly reduced; the abilities of lung cancer cell proliferation, invasion and migration were notably suppressed (P<0.05). In addition, Fibulin-5 overexpression decreased the protein expressions of TLR4, MyD88, p-p65, increased the expression of IκBα (P<0.05). Conclusion: Fibulin-5 may inhibit lung cancer cell proliferation, invasion and migration via inhibiting the expression of HMGB1 and its downstream TLR4/NF-κB pathway.关键词：Fibulin 5;High mobility group box 1;TLR4/NF-κB pathway;Lung cancer;Invasion;Migration2|2552|0更新时间：2025-12-31
- 摘要：Background and purpose: Interleukin-6/signal transducer and activator of transcription 3 (IL-6/STAT3) pathway is overactivated and overexpressed in many malignant tumors, including leukemia, head and neck squamous cell carcinoma, multiple melanoma, breast cancer, and prostate cancer. However, it is rare in the study of large cell lung cancer. The aim of this study was to investigate the effects and mechanisms of IL-6/STAT3 signaling pathway related genes and the downstream genes in large cell lung cancer NL9980 cell lines. Methods: Various concentrations of IL-6 were administered to 5 groups of NL9980 cell lines respectively (0, 1.0, 5.0, 10.0 and 20.0 ng/mL). The changes of proliferation of the NL9980 cell lines were detected by methylthiazol tetrazolium (MTT) assay. Real-time PCR was used to detect mRNA expression changes of the IL-6/STAT3 pathway related genes (including IL-6 and STAT3 genes) and the downstream genes Bcl-2, VEGF, CYCD1. Correlation test with IL-6/STAT3 was done. Results: IL-6 could promote the proliferation of NL9980 cells lines, and the optimal concentration was 5 ng/mL (F=8.11, P<0.05). Various concentrations of IL-6 were administered to NL9980 cell lines. Expressions of IL-6 and STAT3 genes showed obvious increases (4.78±0.09, 5.17±0.05, P<0.05), and the peak level was found in the 5 ng/mL group. The downstream genes Bcl-2, VEGF, CYCD1 also showed obvious increases (4.52±0.14, 4.12±0.12, 3.98±0.17), the peak level was found in the 5 ng/mL group, and other groups did not change significantly (P>0.05). Correlation test showed Bcl-2, VEGF, CYCD1 genes were correlated with IL-6 (r=0.952, r=0.836, r=0.880) and STAT3 (r=0.995, r=0.746, r=0.800) positively. Conclusion: IL-6 could promote the apoptosis of NL9980 cells via activating IL-6/STAT3 signaling pathway and upregulating the downstream genes Bcl-2, VEGF, CYCD1.关键词：Interleukin-6;STAT3;Large cell lung cancer;Mechanism2|1813|0更新时间：2025-12-31
- 摘要：Background and purpose: Papillary thyroid cancer (PTC) is one of the most common carcinomas in humans. Galectin-3 is a multifunctional protein which regulates different process of tumorigenesis. Galectin-3 is widely distributed in various cancer tissues and the sera of cancer patients. The aim of this study was to explore the role of Galectin-3 in promoting PTC cells migration. Methods: Immunohistochemical analyses were performed on 40 PTC tissues and scores were calculated. PTC B-cpap cells were harvested after serum-free culture, and the extracellular and intracellular levels of Galectin-3 were measured. CCK-8 assay and wound healing assay were conducted after inhibition of Galectin-3 with two different methods. The transformation of cell morphology after inhibition of intracellular Galectin-3 was analyzed using immunofluorescence and confocal laser scanning. Results: Compared with those PTC patients without cervical lymph node metastasis, immunohistochemical scores of Galectin-3 were significantly higher in those with cervical lymph node metastases (P<0.05). The inhibition of extracellular and intracellular Galectin-3 attenuated the migration ability of cancer cells, but had different impact on cell proliferation. Transformation of cell morphology was also observed after inhibition of intracellular Galectin-3. Conclusion: Galectin-3 expression in tumor tissues was related to PTC metastasis. Inhibition of extracellular and intracellular galectin-3 could significantly decrease PTC cell migration.关键词：Thyroid neoplasm;Galectin-3;Cell migration assay2|2417|0更新时间：2025-12-31
- 摘要：Background and purpose: Fallopian tube carcinoma is a rare malignant tumor of gynecology with a poor prognosis. In this paper, the clinical and pathological factors affecting the prognosis of fallopian tube carcinoma were analyzed retrospectively. Methods: Effects of clinical and pathological factors on prognosis in 62 patients with fallopian tube carcinoma were retrospectively evaluated. Kaplan-Meier method was used to calculate the survival time, and log-rank test was used for comparison between groups. COX proportional hazards model was used to analyze the significant factors of single factor analysis. Results: The median survival time of 62 patients with fallopian tube carcinoma was 74 months, the 3-year survival rate was 78% and the 5-year survival rate was 63%. International Federation of Gynaecology and Obstetrics (FIGO) stage, postoperative residual tumor size, and number of courses of chemotherapy were the independent prognostic factors in patients with fallopian tube carcinoma. The median survival time, 3-year and 5-year survival rates of P53-negative patients were higher than those of P53-positive patients. The average ratio of Ki-67 was 58.41%, and the median survival time, 3-year and 5-year survival rates of patients with Ki- 67≤58% numerically were higher than those of patients with Ki-67>58%. Conclusion: FIGO stage, postoperative residual tumor size, and number of courses of chemotherapy are the independent prognostic factors in patients with fallopian tube carcinoma. Early diagnosis and early treatment are the key factors to improve the prognosis. Striving for the greatest degree of thoroughness of cytoreductive surgery, followed by an effective and full course of chemotherapy, can improve the survival rate of patients with fallopian tube carcinoma. Pathological examinations, especially the expression levels of p53 protein and Ki-67, have a certain significance in judging the prognosis of fallopian tube carcinoma.关键词：Fallopian tube carcinoma;Clinical;Pathology;Prognosis2|2941|0更新时间：2025-12-31
- 摘要：Background and purpose: Brain tumor is a major fatal neurological disease after cerebral apoplexy. The current study aimed to analyze the incidence and mortality of brain tumors among residents in former Luwan District of Shanghai from Jan. 2004 to Dec. 2011. Methods: The data of brain tumors in permanent residents of former Luwan District were collected from the database of cancer registration and management system in Shanghai. The incidence and mortality of brain tumors were calculated. The rates were standardized by the demographic composition developed in the Fifth Nationwide Census in the year 2000. The temporal trend in the incidence and mortality of brain tumors was assessed. Results: A total of 292 cases of brain tumors were diagnosed from Jan. 2004 to Dec. 2011, accounting for 2.80% of the total patients with malignant diseases. A total of 148 cases died of brain tumors during this period. There were no significant differences in brain tumor incidence and mortality between males and females. There were some fluctuations in the incidence and mortality rates of brain tumors for both males and females from 2004 to 2011. Conclusion: The occurrence of brain tumors and death happened in almost all age categories among permanent residents in former Luwan District of Shanghai from 2004 to 2011. Studying the epidemiology and mechanisms of brain tumor occurrence and progression can aid in the development of effective prevention and treatment strategies, and improve prognosis.关键词：Brain tumors;Incidence;Mortality;Vital statistics2|1750|0更新时间：2025-12-31
- 摘要：Background and purpose: Breast cancer is one of the most common malignant tumors among women. Intravoxel incoherent motion (IVIM) is a functional imaging technique based on the theory of biexponential model developed in recent years, which can exhibit diffusion and perfusion effects. This study aimed to investigate the reproducibility of IVIM parameters and the value in the diagnosis of breast lesions, comparing with quantitative dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) parameters, to explore whether correlations exist among perfusion parameters. Methods: A prospective study was performed in 56 patients with pathologically confirmed benign lesions (n=30), malignant tumors (n=31). All patients underwent DCE-MRI and IVIM imaging. Two radiologists measured the images independently to obtain the parameters from IVIM (D, D*, f ) and DCE-MRI (Ktrans, Kep, Ve). The data consistency between two observers was calculated with intraclass correlation coefficient (ICC). All parameters were statistically compared between benign lesions and malignant tumors using independent sample t test. The receiver operating characteristic (ROC) curve was used to evaluate the ability of the parameters in differentiation of benign and malignant breast lesions. Pearson correlation analyses were used among the IVIM and DCE-MRI parameters. Results: The agreement between 2 radiologists was good for all parameters (ICC≥0.730, P<0.05). D value was significantly lower in malignant lesions than in benign lesions, while the f , Ktrans and Kep values were higher than those in benign lesions (P<0.05). The D* and Ve values showed no differences between benign and malignant breast lesions (P>0.05). The areas under the ROC curve for D, f, Ktrans and Kep were 0.896, 0.677, 0.796 and 0.847, respectively. There was no significant difference in the area under ROC curve between D and Ktrans, neither between the D and Kep (P>0.05). The D value showed higher specificity (86.67%) with the threshold of 1.07×10-3 mm2/s. The Kep value showed higher sensitivity (98.36%) with the threshold of 0.54/min. The f value exhibited a positive correlation with Ktrans and Kep value with r of 0.373 and 0.360 (P<0.05), respectively. D* was also poorly correlated positively with Ktrans with an r of 0.294. Conclusion: The IVIM parameters showed good reproducibility between different radiologists. Both IVIM and DCE-MRI could be helpful in differentiating between benign and malignant breast lesions with positive correlation of the perfusion fraction.关键词：Breast neoplasms;Magnetic resonance imaging;Comparative study;Reproducibility2|1852|0更新时间：2025-12-31
- 摘要：Background and purpose: The apparent diffusion coefficient (ADC) values are the quantitative parameters of diffusion weighted imaging (DWI) which are influenced by many factors, such as b values and region of interest (ROI), and have become the concerns of research. Our study aimed to determine the measurement consistency of ADC in rectal cancer based on different ROIs. Methods: Eighty patients with histologically proven rectal cancer were examined using echo-planar DW MRI values (b value=800 s/mm2). ADC values were measured on ADC map. Mean tumor ADCs were measured according to three distinct slice protocols: ‘whole-slices’,‘single-slice of largest tumor’ and ‘tumor parts of largest independent tumor-containing slices and two adjacent slices’. The freehand ROI was drawn along the border of the high signal of the tumor on the b=800 images to cover the entire tumor area of each slice. Another ROI was drawn using circle tool to cover the entire tumor solid parts as large as possible. After 2 weeks of repeating the measurement between the two physicians. Differences in ADC values were measured using one-way ANOVA. The two protocols were compared for differences in ADC values using paired t-test. We calculated intra- and inter-observer variability using intraclass correlation coefficient (ICC) and Bland-Altman plot. Results: No significant difference was observed by ROIs for three distinct slice protocols (P>0.05). The ADC values obtained by ROIs for the outlined were higher than the ADC values obtained by ROIs for the center analysis (P<0.05). Intraand inter-observer ICC were 0.931, 0.803; 0.913, 0.822 by whole-slices-center and outline ROI respectively. The intraobserver 95% limits of consistency of ADC values were (-0.089 3 to 0.083 1)×10-3mm2/ s (P: Mean=0, P>0.05), (-0.066 8 to 0.096 3)×10-3mm2/s (P<0.05) with center and outline ROI, respectively. The inter-observer 95% limits of consistency of perfusion parameters were (-0.127 5 to 0.141 6)×10-3mm2/s (P>0.05), (-0.112 0 to 0.150 2)×10-3mm2/s (P<0.05) with center and outline ROI, respectively. Bland-Altman plot showed that whole-slices-center ROI could provide more reproducible and stable results. Conclusion: There was no statistically significant difference among three distinct slice protocols analysis of ADC values in rectal cancer. ADC values by whole-slices-center ROI protocol appropriately improved intra- and inter-observer consistency and could provide more reproducible and stable results with less time. ADC values by whole-slices-outlined ROI protocol appropriately indicated the overall situation, but needed more time.关键词：Rectal cancer;Diffusion weighted imaging;Apparent diffusion coefficient;Region of interest2|1811|0更新时间：2025-12-31
- 摘要：Background and purpose: Multifocal/multicentric (MF/MC) breast cancer is frequently encountered in clinical practice. Most studies comparing the heterogeneity of lesions are based on pathological outcomes after upfront surgery. We sought to describe the histological and immunohistochemical characteristics of each lesion in patients with MF/MC breast cancer diagnosed by core needle biopsy. Methods: We retrospectively reviewed the tumor type, grade, and immunohistochemical characteristics ［estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER-2) and Ki-67］ of ipsilateral MF/MC breast cancer patients and assessed the heterogeneity among the lesions. Molecular phenotype was determined by immunohistochemistry. Results: A total of 75 MF/MC breast cancer patients were included. At the time of the manuscript writing, 51 patients proved to be lymph node positive either by fine needle aspiration cytology or sentinel lymph node biopsy. Intertumoral heterogeneity in tumor type and grade was detected in 9 of 75 patients (12.0%). Intertumoral heterogeneity in ER, PR, HER-2 and Ki-67 was detected in 1 (1.3%), 10 (13.3%), 1 (1.3%) and 9 (12.0%) patients, respectively. Intertumoral heterogeneity in molecular phenotype was detected in 10 patients (13.3%). Immunohistochemistry tests carried out according to current guideline may result in suboptimal treatment in 4 patients (5.3%). Conclusion: Histological and immunohistochemical heterogeneity can be found among lesions of ipsilateral MF/MC breast cancer diagnosed by core needle biopsy. Immunohistochemistry tests carried out in the index tumor only may result in suboptimal treatment in these patients.关键词：Breast neoplasms;Multifocality;Multicentricity;Intertumoral heterogeneity;Molecular phenotype2|1977|0更新时间：2025-12-31
- 摘要：Background and purpose: The risk of arm lymphedema in locally advanced breast cancer patients treated with neoadjuvant chemotherapy and local treatment (e.g., surgical procedure and radiation therapy) has not been fully clarified so far. This study was conducted to retrospectively investigate the incidence of lymphedema and associated risk factors in patients treated with neo-adjuvant chemotherapy. Methods: A total of 103 breast cancer patients who underwent neo-adjuvant chemotherapy followed by surgery with axillary lymph node (ALN) dissection from Jan. 2007 to Dec. 2012 were retrospectively analyzed. All patients received postoperative breast/chest wall and supraclavicular field radiation therapy. The determination of lymphedema was based on arm circumference measurement. Results: At a median follow-up time of 4.5 years, 41 patients developed lymphedema. The overall 4.5-year cumulative incidence of lymphedema was 39.8%. According to the analysis result, the number of dissected ALN>15 ［hazard ratio (HR)=2.455; P=0.006］ and having full course of neo-adjuvant chemotherapy (HR=2.199; P=0.014) were independent risk factors for lymphedema. Conclusions: More than 1/3 of the patients in our setting developed lymphedema. Those who have the number of dissected ALN>15 and full course of neo-adjuvant chemotherapy are more likely to develop lymphedema. Thus, more frequent surveillance are necessary for them to detect and treat lymphedema at an early stage.关键词：Breast cancer;Lymphedema;Neoadjuvant chemotherapy;Risk factor2|2507|0更新时间：2025-12-31
- 摘要：With the data generated in recent years, the new issues encountered by practicing physicians in endocrine therapy for breast cancer have been warmly discussed. Meanwhile, the hot discussion brought us new ways of thinking. Based on the new evidence and clinical experience, this article rediscussed the application of ovarian function suppression (OFS) and the appropriate choice of endocrine drugs combining with OFS. We focused on the management of the adverse effects in patients using OFS plus aromatase inhibitor, the fertility problems and prolonged endocrine treatment in breast cancer patients.关键词：Breast cancer;Endocrine therapy;Treatment2|4395|0更新时间：2025-12-31
- 摘要：Renal cancer is one of the most common genitourinary carcers. However, 25% patients present with locally invasive or metastatic renal cancer at the time of diagnosis, and one third of patients who received esection of localized disease will have a recurrence or progression. Recently, immunotherapy for renal cancer has made significant progress. Especially, some immunotherapeutic agents (such as immune checkpoint inhibitor, tumor vaccine) have been proved to improve the prognosis of patients with metastatic renal cancer. In this review, the effects of immunotherapeutic agents in the treatment of renal cancer were summarized.关键词：Metastatic renal cancer;Immunotherapy;Immune checkpoint inhibitor;Tumor vaccine2|3102|0更新时间：2025-12-31
- 摘要：Prostate cancer is one of the most common malignant tumors. Although (prostate-specific antigen, PSA) screening and multidisciplinary therapy had largely improved the therapy effects, new biological markers are needed to achieve individual treatment for prostate cancer. Circulating tumor biomarkers originate from primary tumor tissues and have close relationship with cancer metastases and prognosis. This review summarized the circulating tumor biomarkers detection methods and relevant clinical research in recent years.关键词：Prostate cancer;Circulating tumor biomarkers;Detection;Individual treatment2|2684|0更新时间：2025-12-31
- 关键词：Renal clear cell carcinoma;Metastatic carcinoma of the thyroid gland;Surgery2|3339|0更新时间：2025-12-31
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