The expression of miR-199a-3p in breast cancer and its effect

马大昌; 陈　诚; 吴多明

doi:10.19401/j.cnki.1007-3639.2016.06.001

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The expression of miR-199a-3p in breast cancer and its effect

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- The expression of miR-199a-3p in breast cancer and its effect
- China Oncology Vol. 26, Issue 6, Pages: 481-486(2016)
- 作者机构：
  
  兰州大学第一医院乳腺病科,甘肃,兰州,730000
- 作者简介：
- 基金信息：
- DOI：10.19401/j.cnki.1007-3639.2016.06.001
  CLC：
- Published Online：28 July 2016，
  
  Published：28 July 2016
- 稿件说明：
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马大昌,陈　诚,吴多明,等. miR-199a-3p在乳腺癌中的表达及其作用[J]. 中国癌症杂志, 2016, 26(6): 481-486.

马大昌, 陈　诚, 吴多明. The expression of miR-199a-3p in breast cancer and its effect[J]. China Oncology, 2016, 26(6): 481-486.
马大昌,陈　诚,吴多明,等. miR-199a-3p在乳腺癌中的表达及其作用[J]. 中国癌症杂志, 2016, 26(6): 481-486. DOI： 10.19401/j.cnki.1007-3639.2016.06.001.

马大昌, 陈　诚, 吴多明. The expression of miR-199a-3p in breast cancer and its effect[J]. China Oncology, 2016, 26(6): 481-486. DOI： 10.19401/j.cnki.1007-3639.2016.06.001.

摘要

背景与目的：多种微小RNA(microRNA，miRNA)在乳腺癌中异常表达，在乳腺癌的发生、发展中起重要作用。miRNA可能是治疗乳腺癌的新靶点。该研究旨在探讨miR-199a-3p在乳腺癌中的表达水平，及其对乳腺癌癌细胞增殖和凋亡的影响。方法：运用实时定量聚合酶链式反应(quantitative real-time polymerasechain reaction，QRT-PCR)检测乳腺癌患者癌组织、癌旁正常组织、人乳腺癌细胞和人乳腺细胞中miRNA-199a-3p的表达水平，miR-199a-3p mimic(或inhibitor)转染乳腺癌细胞MDA-MB-231过表达(或沉默)miR-199a-3p的表达后，通过MTT法检测细胞增殖能力，Hoechst染色法和caspase-3活力测试检测细胞凋亡情况。结果：相对癌旁正常组织和人正常乳腺细胞，miR-199a-3p在乳腺癌患者癌组织和人乳腺癌细胞中表达下调。在MDAMB-231中转染miR-199a-3p mimic过表达miR-199a-3p可抑制细胞增殖，促进其凋亡；在MDA-MB-231中转染miR-199a-3p inhibitor沉默miR-199a-3p可促进细胞增殖，抑制其凋亡。结论：miR-199a-3p在乳腺癌中表达下调，并通过调节乳腺癌细胞增殖和凋亡发挥抑癌作用。

Abstract

Background and purpose: Multiple microRNAs (miRNAs) are abnormally expressed in breast cancer and play an important role in the regulation of breast cancer. miRNAs may be a new target for the treatment of breast cancer. This study aimed to investigate the expression of miR-199a-3p in breast cancer and the effect of miR-199a-3p on proliferation and apoptosis of breast cancer. Methods: Real-time PCR was used to test the expression of miR-199a-3p in breast cancer tissues

normal breast tissues

breast cancer cells and normal breast cells. Overexpression (or silencing the expression) of miR-199a-3p was conducted by transfecting MDA-MB-231 with miR-199a-3p mimics (or inhibitors). The proliferation of MDA-MB-231 was detected by MTT method. The apoptosis of MDA-MB-231 was investigated by Hoechst staining and caspase-3 activity assay kit. Results: Compared to corresponding non-tumor breast tissues (or normal breast cell HBL-100)

lower levels of miR-199a-3p were expressed in breast cancer tissues or breast cancer cells. Overexpression of miR-199a-3p induced by miR-199a-3p mimic inhibited the proliferation and promoted the apoptosis of MDA-MB-231

while silencing the expression of miR-199a-3p induced by miR-199a-3p inhibito increased the proliferation and suppressed the apoptosis of MDA-MB-231. Conclusion: The expression of miR-199a-3p is lower in breast cancer

which shows its tumor suppression effect by regulating the proliferation and apoptosis of breast cancer cells.

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