Effect of glucagon-like peptide-1 receptor agonists on growth and energy metabolism in human medullary thyroid cancer cells

王　红; 张斯亮; 关海霞

doi:10.19401/j.cnki.1007-3639.2016.06.002

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Effect of glucagon-like peptide-1 receptor agonists on growth and energy metabolism in human medullary thyroid cancer cells

更新时间：2025-12-31

- Effect of glucagon-like peptide-1 receptor agonists on growth and energy metabolism in human medullary thyroid cancer cells
- China Oncology Vol. 26, Issue 6, Pages: 487-491(2016)
- 作者机构：
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- DOI：10.19401/j.cnki.1007-3639.2016.06.002
  CLC：
- Published Online：28 July 2016，
  
  Published：28 July 2016
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王　红,张斯亮,关海霞. 胰高糖素样肽-1受体激动剂对人甲状腺髓样癌细胞生长和能量代谢的影响[J]. 中国癌症杂志, 2016, 26(6): 487-491.

王　红, 张斯亮, 关海霞. Effect of glucagon-like peptide-1 receptor agonists on growth and energy metabolism in human medullary thyroid cancer cells[J]. China Oncology, 2016, 26(6): 487-491.
王　红,张斯亮,关海霞. 胰高糖素样肽-1受体激动剂对人甲状腺髓样癌细胞生长和能量代谢的影响[J]. 中国癌症杂志, 2016, 26(6): 487-491. DOI： 10.19401/j.cnki.1007-3639.2016.06.002.

王　红, 张斯亮, 关海霞. Effect of glucagon-like peptide-1 receptor agonists on growth and energy metabolism in human medullary thyroid cancer cells[J]. China Oncology, 2016, 26(6): 487-491. DOI： 10.19401/j.cnki.1007-3639.2016.06.002.

摘要

背景与目的：胰高糖素样肽-1(glucagon like peptide-1，GLP-1)受体激动剂是一种新型降糖药。在研发过程中，发现其可增加啮齿类动物患甲状腺C细胞肿瘤的风险。因此，该药物对人类甲状腺的影响引人关注。本研究旨在探讨GLP-1受体激动剂对人甲状腺髓样癌(medullary thyroid cancer，MTC)细胞增殖、降钙素的分泌和能量代谢的影响。方法：体外培养人MTC细胞系(TT)。分别以0、1、10和100 nmol/L艾塞那肽和利拉鲁肽处理细胞24、48和72 h后，采用细胞计数试剂盒(cell counting kit-8，CCK-8)检测GLP-1受体激动剂艾塞那肽和利拉鲁肽处理后细胞增殖情况；采用降钙素试剂盒测定细胞培养上清液中降钙素水平的变化；采用Seahorse能量代谢分析仪检测细胞糖酵解及线粒体呼吸的变化。结果：实验组细胞增殖率与对照组相比，差异无统计学意义(P0.05)，降钙素的量与对照组相比，差异无统计学意义(P0.05)，不同浓度艾塞那肽和利拉鲁肽处理细胞24 h后，与对照组相比，实验组艾塞那肽和利拉鲁肽对MTC细胞能量代谢并无明显影响(P0.05)，随着艾塞那肽和利拉鲁肽处理时间延长，TT细胞糖酵解和线粒体呼吸并无明显改变(P0.05)。结论：GLP-1受体激动剂对人MTC发生、发展无明显促进作用，未来仍需大规模临床数据进一步证实GLP-1受体激动剂的安全性。

Abstract

Background and purpose: This study aimed to investigate the effect of glucagon-like peptide-1 receptor agonists on proliferation

secretion of calcitonin and energy metabolism of medullary thyroid cancer (MTC) cell. Methods: The MTC cell line (TT) was cultured in vitro. After treatment with exenatide and liraglutide (0

10 and 100 nmol/L) for 24

48 and 72 h

the proliferation of TT was analyzed by CCK-8 kit

the calcitonin was measured by calcitonin assay kits

and the energy metabolism of TT was measured by Seahorse XF instrument. Results: When compared with control group

neither exenatide nor liraglutide had effects on proliferation of TT (P0.05); the calcitonin levels did not change significantly after treatment with GLP-1 receptor agonists (P0.05). Exenatide and liraglutide did not alter glycolysis and mitochondrial respiration in TT cells in a dose- and time-dependent manner. Conclusion: GLP-1 receptor agonists have no effect on the development of TT. Further collection of the safety data of exenatide and liraglutide on thyroid is still needed.

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