LncRNA ANCR regulates the biological behavior of gastric cancer cells through miR-331 expression

刘玉华; 魏　蔚; 崔淑萍

doi:10.19401/j.cnki.1007-3639.2019.01.003

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LncRNA ANCR regulates the biological behavior of gastric cancer cells through miR-331 expression

更新时间：2026-01-27

- LncRNA ANCR regulates the biological behavior of gastric cancer cells through miR-331 expression
- China Oncology Vol. 29, Issue 1, Pages: 19-25(2019)
- 作者机构：
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- DOI：10.19401/j.cnki.1007-3639.2019.01.003
  CLC： R735.2
- Published Online：01 February 2019，
  
  Published：01 February 2019
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刘玉华,魏　蔚,崔淑萍. LncRNA ANCR调控miR-331表达影响胃癌细胞的生物学行为[J]. 中国癌症杂志, 2019, 29(1): 19-25.

刘玉华, 魏　蔚, 崔淑萍. LncRNA ANCR regulates the biological behavior of gastric cancer cells through miR-331 expression[J]. China Oncology, 2019, 29(1): 19-25.
刘玉华,魏　蔚,崔淑萍. LncRNA ANCR调控miR-331表达影响胃癌细胞的生物学行为[J]. 中国癌症杂志, 2019, 29(1): 19-25. DOI： 10.19401/j.cnki.1007-3639.2019.01.003.

刘玉华, 魏　蔚, 崔淑萍. LncRNA ANCR regulates the biological behavior of gastric cancer cells through miR-331 expression[J]. China Oncology, 2019, 29(1): 19-25. DOI： 10.19401/j.cnki.1007-3639.2019.01.003.

摘要

背景与目的：胃癌是导致癌症死亡的第二大原因，是东亚、东欧及中南美洲部分地区最常见的胃肠道恶性肿瘤。该研究旨在探讨长链非编码RNA（long-chain noncoding RNA，lncRNA）抗分化非编码RNA（antidifferentiation noncoding RNA，ANCR）靶向miR-331调节胃癌细胞的增殖、侵袭行为及其机制。方法：河南省肿瘤医院2016年1月1日—2016年12月1日获得60例临床胃癌样本和20例对应的癌旁组织标本（距癌组织2 cm）。实时荧光定量聚合酶链反应（real-time fluorescence quantitative polymerase chain reaction，RTFQ-PCR）检测ANCR在胃癌组织和不同胃癌细胞株中的表达情况；分析ANCR的表达和胃癌患者临床病理学特征之间的关系；平板克隆实验检测ANCR对胃癌细胞增殖能力的影响；Transwell侵袭实验检测ANCR对胃癌细胞侵袭能力的影响；裸鼠成瘤实验检测ANCR对胃癌细胞肿瘤异种移植的影响；双荧光素酶报告基因检测ANCR与miR-331之间的相互作用。结果：胃癌组织中ANCR的表达（4.18±0.28）高于正常组织（1.45±0.15），差异有统计学意义（t=12.36，P=0.045）；与其他胃癌细胞株相比，BGC-823和MGC-803细胞中ANCR的表达水平较高（9.12±0.52），差异有统计学意义（P=0.019）；抑制ANCR的表达可以减弱胃癌细胞的增殖能力（98.8±10.4）和侵袭能力（175.9±5.7）；抑制ANCR的表达后胃癌细胞在裸鼠体内异种移植能力（223.4±13.4）弱于NC组（115.6±10.7），差异有统计学意义（t=13.28，P=0.014）；双荧光素酶实验证实ANCR可以直接调控miR-331的表达及荧光活性。结论：ANCR可以靶向调节miR-331的表达影响胃癌细胞的增殖、侵袭行为，影响胃癌细胞在裸鼠体内的生长情况。

Abstract

Background and purpose: Gastric cancer is the second leading cause of cancer death. It is the most common gastrointestinal malignant tumor in East Asia

Eastern Europe and parts of Central and South America. This study aimed to explore the role of long-chain noncoding RNA (lncRNA) antidifferentiation noncoding RNA (ANCR) targeting miR-331 in regulating the proliferation and invasion of gastric cancer cells and its mechanism. Methods: Sixty cases of clinical gastric cancer samples and 20 cases of corresponding non-tumor tissue specimens were collected in Henan Cancer Hospital from January 1

2016 to December 1

2016. The expression levels of ANCR in gastric cancer tissues and different gastric cancer cell lines were detected by real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR). The relationship between the expression of ANCR and the clinicopathological features of gastric cancer patients was analyzed. The effect of ANCR on the proliferation of gastric cancer cells was detected by plate clone assay. Transwell assay was used to detect the effect of ANCR on the invasiveness of gastric cancer cells. Tumor formation assay in nude mouse models detected effect of ANCR on xenograft. The dual luciferase reporter assay detected the interaction between ANCR and miR-331. Results: The expression of ANCR in gastric cancer tissues was significantly higher than that in normal tissues (4.18±0.28 vs 1.45±0.15

t=12.36

P=0.045). Compared with other gastric cancer cell lines

the expression levels of ANCR in BGC-823 and MGC-803 cells were significantly higher (9.12±0.52

t=14.51

P=0.019). Inhibiting the expression of ANCR attenuated the proliferation (98.8±10.4) and invasive ability (175.9±5.7) of the gastric cancer cells. The ability of gastric cancer cells to form xenograft in nude mice was inhibited by suppressing the expression of ANCR (223.4±13.4 vs 115.6±10.7

t=13.28

P=0.014). Dual luciferase assay results confirmed that ANCR could directly regulate the expression of miR-331 and its fluorescence activity. Conclusion: ANCR can regulate the expression of miR-331 and affect the proliferation and invasion of gastric cancer cells.

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