The inhibitory effects of celastrol on proliferation, invasion and migration of human pancreatic cancer cell line PANC-1

高　琦; 郭　艳; 魏小娟

doi:10.19401/j.cnki.1007-3639.2019.01.004

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The inhibitory effects of celastrol on proliferation, invasion and migration of human pancreatic cancer cell line PANC-1

更新时间：2026-01-27

- The inhibitory effects of celastrol on proliferation, invasion and migration of human pancreatic cancer cell line PANC-1
- China Oncology Vol. 29, Issue 1, Pages: 26-31(2019)
- 作者机构：
  
  新乡市中心医院消化内科,河南,新乡,453000
- 作者简介：
- 基金信息：
- DOI：10.19401/j.cnki.1007-3639.2019.01.004
  CLC： R285
- Published Online：01 February 2019，
  
  Published：01 February 2019
- 稿件说明：
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高　琦,郭　艳,魏小娟. 雷公藤红素对人胰腺癌细胞PANC-1增殖、侵袭和迁移的抑制作用[J]. 中国癌症杂志, 2019, 29(1): 26-31.

高　琦, 郭　艳, 魏小娟. The inhibitory effects of celastrol on proliferation, invasion and migration of human pancreatic cancer cell line PANC-1[J]. China Oncology, 2019, 29(1): 26-31.
高　琦,郭　艳,魏小娟. 雷公藤红素对人胰腺癌细胞PANC-1增殖、侵袭和迁移的抑制作用[J]. 中国癌症杂志, 2019, 29(1): 26-31. DOI： 10.19401/j.cnki.1007-3639.2019.01.004.

高　琦, 郭　艳, 魏小娟. The inhibitory effects of celastrol on proliferation, invasion and migration of human pancreatic cancer cell line PANC-1[J]. China Oncology, 2019, 29(1): 26-31. DOI： 10.19401/j.cnki.1007-3639.2019.01.004.

摘要

背景与目的：雷公藤红素（celastrol）是雷公藤的主要活性成分，现代研究发现其具有广泛的抗癌活性，对胰腺癌细胞凋亡有一定的促进作用。该研究旨在探讨celastrol对胰腺癌细胞PANC-1增殖、侵袭和迁移的作用。方法：将PANC-1细胞分为PANC-1组、celastrol（5 μmol/L）组、celastrol（10 μmol/L）组和celastrol（20 μmol/L）组，分别用0、5、10和20 μmol/L的celastrol处理PANC-1细胞，细胞计数试剂盒（cell counting kit-8，CCK-8）检测细胞增殖倍数，Transwell检测各组PANC-1细胞的侵袭能力，划痕实验检测各组细胞迁移能力。蛋白质印迹法（Western blot）检测Ki-67、血管内皮生长因子（vascular endothelial growth factor，VEGF）和基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9）的蛋白表达水平。结果：与PANC-1组比较，celastrol（5 μmol/L）组、celastrol（10 μmol/L）组和celastrol（20 μmol/L）组细胞增殖倍数明显降低，细胞增殖标记蛋白Ki-67的蛋白表达水平与PANC-1组比较也明显降低；同时，celastrol（5 μmol/L）组、celastrol（10 μmol/L）组和celastrol（20 μmol/L）组侵袭细胞数与PANC-1组相比明显减少；此外，与PANC-1组比较，celastrol（5 μmol/L）组、celastrol（10 μmol/L）组和celastrol（20 μmol/L）组划痕闭合率显著降低，VEGF和MMP-9的蛋白表达水平也显著低于PANC-1组。结论：Celastrol能抑制人胰腺癌细胞PANC-1增殖、侵袭及迁移。

Abstract

Background and purpose: Celastrol is the primary active component of Tripterygium wilfordii. The present studies indicate that celastrol displays extensive antitumor activity and promotes apoptosis in pancreatic cancer. This study aimed to investigate the effect of celastrol on cell proliferation

invasion and migration of human pancreatic cancer cell line PANC-1. Methods: PANC-1 cells were divided into PANC-1 group

celastrol (5 μmol/L) group

celastrol (10 μmol/L) group and celastrol (20 μmol/L) group

and the cells were treated with different levels of celastrol (5

10 and 20 μmol/L). Cell counting kit-8 (CCK-8) was used to evaluate cell proliferation. The invasion ability was determined using Transwell assay

and cell migration ability was determined by wound healing assay. The expressions of proteins

including Ki-67

vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) were measured by Western blot. Results: Compared with PANC-1 group

the cell proliferation was decreased in celastrol (5 μmol/L) group

celastrol (10 μmol/L) group and celastrol (20 μmol/L) group

and the protein level of Ki-67 was decreased significantly in celastrol-treated groups compared with PANC-1 group. Meanwhile

cell invasion was decreased in celastrol (5 μmol/L) group

celastrol (10 μmol/L) group and celastrol (20 μmol/L) group compared with PANC-1 group. In addition

compared with PANC-1 group

the wound closure rates in celastrol (5 μmol/L) group

celastrol (10 μmol/L) group and celastrol (20 μmol/L) group were decreased notably

and the protein levels of VEGF and MMP-9 were decreased compared with PANC-1 group. Conclusion: Celastrol can inhibit cell proliferation

invasion and migration of human pancreatic cancer cell line PANC-1.

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