Progress of single-cell sequencing in solid tumors

郑小翠; 汪希鹏

doi:10.19401/j.cnki.1007-3639.2019.07.009

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Progress of single-cell sequencing in solid tumors

更新时间：2026-01-27

- Progress of single-cell sequencing in solid tumors
- China Oncology Vol. 29, Issue 7, Pages: 535-539(2019)
- 作者机构：
  
  上海交通大学医学院附属新华医院妇产科,上海,200092
- 作者简介：
- 基金信息：
- DOI：10.19401/j.cnki.1007-3639.2019.07.009
  CLC： R730.4
- Published Online：12 July 2019，
  
  Published：12 July 2019
- 稿件说明：
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郑小翠,汪希鹏 . 单细胞测序技术在实体瘤研究中的应用进展[J]. 中国癌症杂志, 2019, 29(7): 535-539.

郑小翠, 汪希鹏. Progress of single-cell sequencing in solid tumors[J]. China Oncology, 2019, 29(7): 535-539.
郑小翠,汪希鹏 . 单细胞测序技术在实体瘤研究中的应用进展[J]. 中国癌症杂志, 2019, 29(7): 535-539. DOI： 10.19401/j.cnki.1007-3639.2019.07.009.

郑小翠, 汪希鹏. Progress of single-cell sequencing in solid tumors[J]. China Oncology, 2019, 29(7): 535-539. DOI： 10.19401/j.cnki.1007-3639.2019.07.009.

摘要

肿瘤异质性是实体瘤组织的重要特征之一，其多样性和复杂性给实体瘤的基础研究和临床诊治带来了极大的困难。因此，鉴别实体瘤内及其微环境中不同的细胞亚群及与之相关的基因表达水平就显得十分重要。单细胞测序技术是利用DNA二代测序技术对单个细胞的DNA、RNA或DNA甲基化水平进行分析，分别揭示其基因组、转录组和表观遗传学特征，了解单个细胞功能和所处的状态。单细胞测序分析不仅能进一步鉴定实体瘤内细胞的异质性，还有助于阐明肿瘤发生、发展、转移、耐药和免疫逃逸等分子作用机制，从而使实体瘤的临床诊治和转归预测变得更加准确。简要总结目前单细胞测序技术的发展，对单细胞测序在实体瘤研究中的应用与进展进行综述。

Abstract

Heterogeneity is one of the most important characteristics of solid tumors. Therefore

it is essential to identify different cell subsets and related gene expression levels in solid tumor and its microenvironment. Single-cell sequencing uses DNA second-generation sequencing technology to analyze the DNA

RNA or DNA methylation level of a single cell

reveal its genome

transcriptome and epigenetic characteristics

and understand the state and function of a single cell. Single-cell sequencing can not only further identify the heterogeneity of cells in solid tumors

but also help clarify the molecular mechanisms of tumor development

metastasis

drug resistance and immune escape

making clinical diagnosis

treatment and prognosis of solid tumors more accurate. This article briefly summarized the advances of single-cell sequencing

and reviewed the application and progress of single-cell sequencing in solid tumor research.

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