Background and purpose: At present

the incidence of primary breast cancer in female populations in China is gradually increasing

which has a great impact on women's physical and mental health. Therefore

the characteristics of malignant calcified mammography and their correlation with expressions of vascular endothelial growth factor (VEGF) and E-cadherin in primary breast cancer were systematically analyzed to provide targeted guidance for clinical diagnosis and treatment. Methods: A retrospective analysis of 243 patients with primary breast cancer confirmed by surgery and pathology was performed in Changzhou Second People’s Hospital from Mar. 2015 to Apr. 2019. All patients received mammography before surgery. And after the operation

pathological and immunohistochemical examinations were carried out

and the surgical and pathological results were used as the gold standard to analyze the diagnosis results of this group. Results: In this group of 243 patients

there were 118 cases with malignant calcification and 125 cases without malignant calcification. The results showed that the Twist protein was significantly correlated with breast cancer malignant calcification

morphology and quantity. Besides

the Twist protein expression was positively correlated with the VEGF expression in breast cancer tissues (P0.05)

and the positive expression of Twist protein was negatively correlated with the positive expression of E-cadherin (P0.05). Conclusion: The expressions of Twist protein

VEGF and E-cadherin can provide a biological basis for the diagnosis of malignant calcified molybdenum and palladium in breast cancer

which has clinical significance.