Genetic variation in membrane cofactor protein CD46 rs1970530 affects the risk of hepatocellular carcinoma

徐珊珊; 宋琴琴; 仵红娇; 付　宁; 车玲玉; 金　敏; 刘　冲; 韩素桂; 张雪梅; 张　志

doi:10.19401/j.cnki.1007-3639.2020.09.003

您当前的位置：

首页 >

文章列表页 >

Genetic variation in membrane cofactor protein CD46 rs1970530 affects the risk of hepatocellular carcinoma

更新时间：2026-01-27

- Genetic variation in membrane cofactor protein CD46 rs1970530 affects the risk of hepatocellular carcinoma
- China Oncology Vol. 30, Issue 9, Pages: 656-660(2020)
- 作者机构：
  
  1. 华北理工大学附属唐山市工人医院肿瘤科,河北,唐山,063000
  2. 华北理工大学生命科学学院,河北,唐山,063210
  3. 华北理工大学附属唐山市人民医院核医学检验科,河北,唐山,063000
- 作者简介：
- 基金信息：
- DOI：10.19401/j.cnki.1007-3639.2020.09.003
  CLC： R735.7
- Published Online：10 October 2020，
  
  Published：10 October 2020
- 稿件说明：
移动端阅览
徐珊珊 , 宋琴琴 , 仵红娇 , 付　宁 , 车玲玉 , 金　敏 , 刘　冲 , 韩素桂 , 张雪梅 , 张　志 . 膜辅助蛋白CD46 rs1970530遗传变异对肝癌发病风险的影响[J]. 中国癌症杂志, 2020, 30(9): 656-660.

徐珊珊, 宋琴琴, 仵红娇, et al. Genetic variation in membrane cofactor protein CD46 rs1970530 affects the risk of hepatocellular carcinoma[J]. China Oncology, 2020, 30(9): 656-660.
徐珊珊 , 宋琴琴 , 仵红娇 , 付　宁 , 车玲玉 , 金　敏 , 刘　冲 , 韩素桂 , 张雪梅 , 张　志 . 膜辅助蛋白CD46 rs1970530遗传变异对肝癌发病风险的影响[J]. 中国癌症杂志, 2020, 30(9): 656-660. DOI： 10.19401/j.cnki.1007-3639.2020.09.003.

徐珊珊, 宋琴琴, 仵红娇, et al. Genetic variation in membrane cofactor protein CD46 rs1970530 affects the risk of hepatocellular carcinoma[J]. China Oncology, 2020, 30(9): 656-660. DOI： 10.19401/j.cnki.1007-3639.2020.09.003.

摘要

背景与目的：膜辅助蛋白CD46可保护宿主细胞免受补体依赖的细胞毒性作用，研究表明，CD46可能作为肝细胞癌（hepatocellular carcinoma，HCC）患者的潜在生物标志物。探讨CD46基因表达及启动子区遗传变异与HCC发病风险的关系。方法：通过基因表达谱交互分析（gene expression profiling interactive analysis，GEPIA）在线网站分析HCC组织和正常肝组织CD46表达的差异；2011—2015年在华北理工大学附属唐山市工人医院和华北理工大学附属唐山市人民医院经病理学检查确诊且未经放化疗的240例HCC患者作为病例组，对照组为同时期入院体检的500名健康人群。采用聚合酶链反应-限制性片段长度多态性分析（polymerase chain reaction-restriction fragment length polymorphism，PCR-RFLP）法进行基因分型，检测两组基因型频率和等位基因频率，评估CD46 rs1970530遗传变异与HCC发病风险的关系。结果：CD46在HCC组织与正常组织中的表达差异有统计学意义（P0.05）。经非条件logistic回归分析发现，CD46 rs1970530至少携带一个G等位基因型在病例组及对照组之间差异有统计学意义（OR=0.666，95% CI：0.448~0.990，P0.05）；两组间等位基因G频率差异有统计学意义（OR=0.689，95% CI：0.478~0.994，P0.05）。分层分析结果显示，至少携带一个G等位基因者可降低高年龄组（60岁）（P=0.048）和男性（P=0.023）人群的HCC发病风险，在女性和低年龄组（≤60岁）中差异无统计学意义（P0.05）。按吸烟状态进行分层分析，rs1970530变异与HCC易感性无明显关联（P0.05）。结论：HCC中CD46基因高表达及CD46 rs1970530遗传变异影响HCC发病风险。

Abstract

Background and purpose: Membrane cofactor protein CD46 protects host cells from complement-dependent cytotoxicity and may be a potential molecular marker for hepatocellular carcinoma (HCC). This study aimed to investigate the correlation between the gene expression of CD46 with the genetic variation of CD46 promoter and the risk of HCC. Methods: The difference in CD46 expression between HCC tissues and normal liver tissues was analyzed by gene expression profiling interactive analysis (GEPIA) online website. From 2011 to 2015

240 HCC patients were confirmed by pathological diagnosis in Tangshan Gongren Hospital

North China University of Science and Technology and Tangshan People’s Hospital

North China University of Science and Technology

and 500 healthy people enrolled as the control group in the same period. Genotyping was performed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to detect genotype frequencies and allele frequencies between two groups. The relationship between the genetic variation of CD46 rs1970530 and the risk of HCC was evaluated. Results: The expression of CD46 was significantly different between HCC tissues and normal tissues (P0.05). Unconditional logistic regression analysis indicated the difference in CD46 rs1970530 genotype carrying at least one G allele was statistically significant between the case group and the control group (OR=0.666

95% CI: 0.448-0.990

P0.05)

and there was also a significant difference in the frequency of the allele G (OR=0.689

95% CI: 0.478-0.994

P0.05). The stratification analysis showed that CD46 rs1970530 genotype carrying at least one G allele could reduce the risk of HCC in the advanced age group (60 years) (P=0.048) and men (P=0.023)

however

there was no difference in women and younger age groups between the HCC and the healthy control (≤60 years) (P0.05). Smoking stratification analysis indicated that there was no significant correlation between rs1970530 mutation and HCC susceptibility (P0.05). Conclusion: The high gene expression of CD46 in HCC and the genetic variation of CD46 rs1970530 affect the risk of HCC.

关键词

Keywords

references

Views

1432

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

PARP1 promotes the progression of hepatocellular carcinoma by regulating expression of POU2F2

Significance of infiltration of M1 tumor-associated macrophages in hepatocellular carcinoma

Progress in research, diagnosis, and treatment of hepatocellular carcinoma in 2022

Mechanism of LINC00601 in regulating sensitivity of hepatocellular carcinoma cells to oxaliplatin chemotherapy

Plasma exosomal CD48 protein as a candidate diagnostic biomarker for hepatocellular carcinoma

Related Author

Ziqiang WEN

Junliang LAN

Bo ZHOU

Qiwei XU

Feng XIAO

Tonglin XU

Lin ZHU

Jingwen XIAO

Related Institution

Department of Hepatobiliary Surgery, Linfen People’s Hospital, The Seventh Clinical Medical College of Shanxi Medical University

Clinical Medicine, Major of Medical College Nantong University

Department of Surgery, the Third People’s Hospital of Nantong, Affiliated Nantong Hospital Three of Nantong University

Department of Pathology, the Third People's Hospital of Nantong, Affiliated Nantong Hospital Three of Nantong University

Liver Cancer Institute, Zhongshan Hospital, Fudan University

AI问答

⁰