Advances in the application of co-culture strategies in organoids

Yu LU; Yumeng XI; Xiaoming HE; Shaokun YANG; Jia ZHANG; Lei WANG; Chaoxing HE; Bai XIANG

doi:10.19401/j.cnki.1007-3639.2023.03.014

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Advances in the application of co-culture strategies in organoids

Review | 更新时间：2025-12-31

- Advances in the application of co-culture strategies in organoids
- China Oncology Vol. 33, Issue 3, Pages: 293-302(2023)
- 作者机构：
  
  1. 河北医科大学药学院，河北石家庄 050017
  2. 石家庄市第四医院药剂科，河北石家庄 050035
  3. 河北医科大学第四医院胸外科，河北石家庄 050011
- 作者简介：
- 基金信息：
- DOI：10.19401/j.cnki.1007-3639.2023.03.014
  CLC： R73-35+1
- Received：13 May 2022，
  
  Revised：2022-08-05，
  
  Published：30 March 2023
- 稿件说明：
移动端阅览
Yu LU, Yumeng XI, Xiaoming HE, et al. Advances in the application of co-culture strategies in organoids[J]. China Oncology, 2023, 33(3): 293-302.
DOI：

Yu LU, Yumeng XI, Xiaoming HE, et al. Advances in the application of co-culture strategies in organoids[J]. China Oncology, 2023, 33(3): 293-302. DOI： 10.19401/j.cnki.1007-3639.2023.03.014.

摘要

类器官作为一种良好的体外研究模型，在生物医学领域中的应用越来越广泛。通过采用各种组织培养技术开发自组装的3D结构，类器官可重现器官固有结构中细胞的高度复杂性，因此被广泛用于研究调节机体发育和疾病的机制、高通量药物筛选及个性化治疗等。为更好地重现微环境内细胞间的相互作用，共培养策略已经扩展到更多的细胞类型，共培养策略的迅速发展使类器官技术的应用前景更加广阔，并为治疗人类疾病和再生医学开辟了新的道路。本文阐述共培养策略在类器官生成中的作用，并重点介绍不同细胞成分及微生物组与类器官共培养的应用，以期为构建开发具有更高体内模拟程度的类器官提供参考和帮助。

Abstract

As a good in vitro research model

organoids are more and more widely used in the biomedical field. By developing self-assembled 3D structures using various tissue culture techniques

organoids can rebuild the high complexity of cells in the inherent structure of the organ

and are therefore unanimously used to study mechanisms regulating body development and disease

high-throughput drug screening

and personalized treatment and so on. To better reca

pitulate cell-to-cell interactions within the microenvironment

co-culture strategies have been extended to more cell types

and their rapid development offers broader prospects for organoids and paves the way for the treatment of human diseases and regenerative medicine. This review discussed the role of co-culture strategies in organoid generation

and focused on the application of various cellular components and microorganisms in organoid construction

thereby providing reference and help for scholars to construct and develop organoids with a higher degree of

in vivo

simulation.

关键词

Keywords

references

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