Mechanism of circular RNA hsa_circ_0012779 expression in nasopharyngeal carcinoma and its influence on cell biological behavior

Pingchuan ZHANG; Mingyu DU; Chengyun YAO; Xia HE; Li YIN

doi:10.19401/j.cnki.1007-3639.2023.05.004

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Mechanism of circular RNA hsa_circ_0012779 expression in nasopharyngeal carcinoma and its influence on cell biological behavior

Article | 更新时间：2025-12-31

- Mechanism of circular RNA hsa_circ_0012779 expression in nasopharyngeal carcinoma and its influence on cell biological behavior
- China Oncology Vol. 33, Issue 5, Pages: 445-451(2023)
- 作者机构：
  
  1. 南京医科大学附属肿瘤医院，江苏省肿瘤医院，江苏省肿瘤防治研究所放疗科，江苏南京 210009
  2. 南京医科大学第四临床医学院，江苏南京 210000
- 作者简介：
  
  YIN Li.
- 基金信息：
- DOI：10.19401/j.cnki.1007-3639.2023.05.004
  CLC： R739.6
- Received：04 November 2022，
  
  Revised：2023-03-04，
  
  Published：30 May 2023
- 稿件说明：
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Pingchuan ZHANG, Mingyu DU, Chengyun YAO, et al. Mechanism of circular RNA hsa_circ_0012779 expression in nasopharyngeal carcinoma and its influence on cell biological behavior[J]. China Oncology, 2023, 33(5): 445-451.
DOI：

Pingchuan ZHANG, Mingyu DU, Chengyun YAO, et al. Mechanism of circular RNA hsa_circ_0012779 expression in nasopharyngeal carcinoma and its influence on cell biological behavior[J]. China Oncology, 2023, 33(5): 445-451. DOI： 10.19401/j.cnki.1007-3639.2023.05.004.

摘要

背景与目的：

环状RNA（circular RNA，circRNA）在多种肿瘤的发展过程中发挥重要的调节作用。然而，circRNA在鼻咽癌中的异常表达和生物学功能仍不清楚。本研究旨在探讨hsa_circ_0012779对鼻咽癌细胞生物学行为的影响及其分子机制。

方法：

通过实时荧光定量聚合酶链反应（real-time fluorescence quantitative polymerase chain reaction，RTFQ-PCR）检测正常人鼻咽上皮细胞NP69及鼻咽癌细胞系CNE2、5-8F、HNE1、SUNE1中hsa_circ_0012779的表达。采用细胞计数试剂盒-8（cell counting kit-8，CCK-8）实验和transwell侵袭实验检测hsa_circ_0012779对鼻咽癌细胞增殖和侵袭能力的影响。采用蛋白质印迹法（Western blot）检测hsa_circ_0012779对ELAV样蛋白1（ELAV like protein 1，ELAVL1）表达的影响，通过RNA下拉实验验证hsa_circ_0012779与ELAVL1的直接结合。

结果：

hsa_circ_0012779在鼻咽癌组织和细胞中高表达，敲减hsa_circ_0012779能抑制鼻咽癌细胞增殖和侵袭，hsa_circ_0012779与RNA结合蛋白ELAVL1结合促进其表达并且共定位于细胞质中。同时，敲减hsa_circ_0012779对鼻咽癌细胞的影响能被ELAVL1过表达逆转。

结论：

hsa_circ_0012779促进ELAVL1的表达，进而促进鼻咽癌细胞增殖和侵袭，影响鼻咽癌的发生、发展。

Abstract

Background and purpose:

Circular RNA (circRNA) plays an important regulatory role in the development of a variety of tumors. However

the abnormal expression and biological function of circRNA in nasopharyngeal carcinoma remain unclear. This study aimed to explore the effect of hsa_circ_0012779 on the biological behavior of nasopharyngeal carcinoma cells and its molecular mechanism.

Methods:

The expression of hsa_circ_0012779 in human immortalized nasopharyngeal epithelial cell line NP69 and nasopharyngeal carcinoma cell lines CNE2

5-8F

HNE1 and SUNE1 was detected by real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR). Cell counting kit-8 (CCK-8) assay and transwell invasion assay were used to detect the effect of hsa_circ_0012779 on the proliferation and invasion ability of nasopharyngeal carcinoma cells. The protein level of ELAV like pr

otein 1 (ELAVL1) in NPC cells with hsa_circ_0012779 knockdown was detected by Western blot. The binding of hsa_circ_0012779 and ELAVL1 was verified by RNA pull-down assay.

Results:

hsa_circ_0012779 was highly expressed in nasopharyngeal carcinoma tissues and cells. Knockdown hsa_circ_0012779 could inhibit the proliferation and invasion of nasopharyngeal carcinoma cells. hsa_circ_0012779 bound to RNA-binding protein ELAVL1 to promote its expression and colocalization in cytoplasm. In the meanwhile

the effect of knockdown hsa_circ_0012779 on nasopharyngeal carcinoma cells could be reversed by the overexpression of ELAVL1.

Conclusion:

hsa_circ_0012779 promote the expression of ELAVL1 and thus promote the proliferation and invasion of nasopharyngeal carcinoma

and influence the occurrence and development of nasopharyngeal carcinoma.

关键词

Keywords

references

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