Effect of long noncoding RNA FLJ30679 on proliferation and migration of oral squamous cell carcinoma cells

Rongqi SUN; Ning SONG; Wentian ZHENG; Xinyue ZHANG; Minmin LI; Hui GONG; Yingying JIANG

doi:10.19401/j.cnki.1007-3639.2024.05.001

您当前的位置：

首页 >

文章列表页 >

Effect of long noncoding RNA FLJ30679 on proliferation and migration of oral squamous cell carcinoma cells

Article | 更新时间：2025-12-31

- Effect of long noncoding RNA FLJ30679 on proliferation and migration of oral squamous cell carcinoma cells
- China Oncology Vol. 34, Issue 5, Pages: 439-450(2024)
- 作者机构：
  
  山东第二医科大学口腔医学院，山东潍坊 261053
- 作者简介：
- 基金信息：
  
  Shandong Provincial Natural Science Foundation(ZR2023LSW019)
- DOI：10.19401/j.cnki.1007-3639.2024.05.001
  CLC： R739.85
- Received：07 October 2023，
  
  Revised：2024-02-20，
  
  Published：30 May 2024
- 稿件说明：
移动端阅览
Rongqi SUN, Ning SONG, Wentian ZHENG, et al. Effect of long noncoding RNA FLJ30679 on proliferation and migration of oral squamous cell carcinoma cells[J]. China Oncology, 2024, 34(5): 439-450.
DOI：

Rongqi SUN, Ning SONG, Wentian ZHENG, et al. Effect of long noncoding RNA FLJ30679 on proliferation and migration of oral squamous cell carcinoma cells[J]. China Oncology, 2024, 34(5): 439-450. DOI： 10.19401/j.cnki.1007-3639.2024.05.001.

摘要

背景与目的：

长链非编码RNA（long noncoding RNA，lncRNA）能够调控基因转录、mRNA剪切、稳定和翻译，是多种生物学过程中的重要调节因子。本研究旨在探讨lncRNA FLJ30679在口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）中的表达、与临床病理学特征的关系及对OSCC恶性生物学行为的影响。

方法：

通过UCSC Xena数据库分析FLJ30679在头颈部鳞状细胞癌（head and neck squamous cell carcinoma，HNSCC）组织中的表达及与临床病理学特征的关系。通过实时荧光定量聚合酶链反应（real-time fluorescence quantitative polymerase chain reaction，RTFQ-PCR）检测FLJ30679在OSCC细胞系中的表达，通过RNA核质分离实验明确其亚细胞定位；采用FLJ30679 Smart Silencer建立FLJ30679敲减组（SS-FLJ30679），采用过表达质粒建立FLJ30679过表达组（FLJ30679）。通过细胞计数试剂盒-8（cell counting kit-8，CCK-8）和transwell迁移实验检测FLJ30679表达改变对OSCC细胞增殖和迁移能力的影响。通过RTFQ-PCR和蛋白质印迹法（Western blot）检测OSCC细胞中的FLJ30679表达改变对上皮-间充质转化（epithelial-mesenchymal transition，EMT）相关基因表达的影响。通过Western blot检测FLJ30679表达改变对磷脂酰肌醇3-激酶（phosphoinositide 3-kinase，PI3K）/蛋白激酶B（protein kinase，AKT）通路的影响。

结果：

数据库分析显示，FLJ30679在HNSCC组织中的表达高于正常组织（

0.01），且其表达与不良预后有关（

0.01）。FLJ30679在6种OSCC细胞系中均高表达，且主要定位于细胞核。与对照组相比，FLJ30679敲减组的OSCC细胞增殖和迁移能力显著降低（

0.01），FLJ30679过表达组的OSCC细胞增殖和迁移能力显著升高（

0.001）。FLJ30679敲减导致E-钙黏蛋白（E-cadherin）的mRNA和蛋白表达水平上调（

0.01），而N-钙黏蛋白（N-cadherin）和波形蛋白（vimentin）的mRNA和蛋白表达水平下调（

0.01）；FLJ30679过表达导致E-cadherin的蛋白表达水平下调（

0.001），而N-cadherin和vimentin的mRNA和蛋白表达水平上调（

0.05）。FLJ30679敲减导致磷酸化PI3K（phosphorylated-PI3K，p-PI3K）和磷酸化AKT（phosphorylated-AKT，p-AKT）的蛋白表达水平下调（

0.001），FLJ30679过表达导致p-PI3K和p-AKT的蛋白表达水平上调（

0.01）。

结论：

FLJ30679在OSCC细胞和组织中表达上调，能够促进OSCC细胞增殖和迁移，这可能与FLJ30679激活PI3K/AKT通路，促进EMT的发生有关。

Abstract

Background and purpose:

Long noncoding RNA (lncRNA) can regulate gene transcription

mRNA shear

stabilization and translation

and it is an important regulatory factor in a var

iety of biological processes. This study aimed to investigate the expression and clinical features of lncRNA FLJ30679 in oral squamous cell carcinoma (OSCC) and its effect on the malignant biological behavior of OSCC.

Methods:

The expression of FLJ30679 in head and neck squamous cell carcinoma (HNSCC) tissues and normal tissues was analyzed by the UCSC Xena database for expression and prognosis. The expression of FLJ30679 in OSCC cell lines was detected by real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR). The subcellular localization of FLJ30679 in OSCC cells was detected by RNA nuclear-cytoplasmic fractionation assays. FLJ30679 Smart Silencer was used to establish the FLJ30679 knockdown group (SS-FLJ30679)

and overexpression plasmid of FLJ30679 was used to establish FLJ30679 overexpression group (FLJ30679). The effects of altered FLJ30679 expression on the proliferative and migration capacity of OSCC cells were examined by cell counting kit-8 (CCK-8) and transwell migration assays. RTFQ-PCR and Western blot were used to determine the effect of altered FLJ30679 expression on the expression of epithelial-mesenchymal transition (EMT)-related genes in OSCC cells. The effects of altered FLJ30679 expression on the phosphoinositide 3-kinase (PI3K)/protein kinase (AKT) pathway were detected by Western blot.

Results:

Online query of database showed that FLJ30679 expression was higher in HNSCC tissues compared to normal tissues (

0.01). HNSCC patients with higher FLJ30679 expression had lower overall survival (

0.01). The RTFQ-PCR results showed that FLJ30679 was expressed at a higher level in six OSCC cell lines compared with normal cells

and was predominantly localized in the nucleus. The ability of OSCC cells in the SS-FLJ30679 group to proliferate and migrate was significantly lower compared with the SS-NC group (

0.01). OSCC cells in the FLJ30679 overexpression group had significantly higher proliferativ

e and migratory capacities than those in the vector group (

0.001). RTFQ-PCR and Western blot results showed that FLJ30679 knockdown resulted in upregulation of mRNA and protein expression levels of E-cadherin (

0.01) and downregulation of mRNA and protein expression levels of N-cadherin and vimentin (

0.01). FLJ30679 overexpression resulted in downregulation of protein expression levels of E-cadherin (

0.01) and upregulation of mRNA and protein expression levels of N-cadherin and vimentin (

0.05). Western blot results showed that knockdown of FLJ30679 resulted in decreased protein expression levels of phosphorylated-PI3K (p-PI3K) and phosphorylated-AKT (p-AKT) (

0.001)

and overexpression of FLJ30679 resulted in increased protein expression levels of p-PI3K and p-AKT (

0.01).

Conclusion:

The expression of FLJ30679 was increased in OSCC tissues and cells. It promoted the proliferation and migration ability of OSCC cells

which may be caused by FLJ30679 promoting EMT via PI3K/AKT pathway.

关键词

Keywords

references

CHAI A W Y , LIM K P , CHEONG S C . Translational genomics and recent advances in oral squamous cell carcinoma [J ] . Semin Cancer Biol , 2020 , 61 : 71 - 83 . DOI: S1044-579X(19)30260-3 http://doi.org/S1044-579X(19)30260-3

PANARESE I , AQUINO G , RONCHI A , et al. Oral and oropharyngeal squamous cell carcinoma: prognostic and predictive parameters in the etiopathogenetic route [J ] . Expert Rev Anticancer Ther , 2019 , 19 ( 2 ): 105 - 119 .

HE J Y , YE W , KOU N , et al. MicroRNA-29b-3p suppresses oral squamous cell carcinoma cell migration and invasion via IL32/AKT signalling pathway [J ] . J Cell Mol Med , 2020 , 24 ( 1 ): 841 - 849 . DOI: 10.1111/jcmm.14794 http://doi.org/10.1111/jcmm.14794

KURIHARA-SHIMOMURA M , SASAHIRA T , NAKAMURA H , et al. Zinc finger AN1-type containing 4 is a novel marker for predicting metastasis and poor prognosis in oral squamous cell carcinoma [J ] . J Clin Pathol , 2018 , 71 ( 5 ): 436 - 441 .

KOPP F , MENDELL J T . Functional classification and experimental dissection of long noncoding RNAs [J ] . Cell , 2018 , 172 ( 3 ): 393 - 407 . DOI: S0092-8674(18)30048-5 http://doi.org/S0092-8674(18)30048-5

ULITSKY I , BARTEL D P . lincRNAs: genomics, evolution, and mechanisms [J ] . Cell , 2013 , 154 ( 1 ): 26 - 46 . DOI: 10.1016/j.cell.2013.06.020 http://doi.org/10.1016/j.cell.2013.06.020

GUTTMAN M , RINN J L . Modular regulatory principles of large non-coding RNAs [J ] . Nature , 2012 , 482 ( 7385 ): 339 - 346 .

PUDERECKI M , SZUMIŁO J , MARZEC-KOTARSKA B . Novel prognostic molecular markers in lung cancer [J ] . Oncol Lett , 2020 , 20 ( 1 ): 9 - 18 . DOI: 10.3892/ol.2020.11541 http://doi.org/10.3892/ol.2020.11541

CHEN M W , LIU B Q , XIAO J B , et al. A novel seven-long non-coding RNA signature predicts survival in early stage lung adenocarcinoma [J ] . Oncotarget , 2017 , 8 ( 9 ): 14876 - 14886 . DOI: 10.18632/oncotarget.14781 http://doi.org/10.18632/oncotarget.14781

GOLDMAN M J , CRAFT B , HASTIE M , et al. Visualizing and interpreting cancer genomics data via the Xena platform [J ] . Nat Biotechnol , 2020 , 38 ( 6 ): 675 - 678 . DOI: 10.1038/s41587-020-0546-8 http://doi.org/10.1038/s41587-020-0546-8

蒋英英 , 陈曦 , 石雨 , 等 . 长链非编码RNA COL11A1-208对口腔鳞癌细胞增殖及侵袭的影响 [J ] . 解放军医学杂志 , 2022 , 47 ( 9 ): 851 - 862 .

JIANG Y Y , CHEN X , SHI Y , et al. Effect of long non-coding RNA COL11A1-208 on proliferation and invasion in oral squamous cell carcinoma cells [J ] . Med J Chin People’s Liberation Army , 2022 , 47 ( 9 ): 851 - 862 .

LI J H , LIU S , ZHOU H , et al. starBase v2.0: decoding miRNA-ceRNA, miRNA-ncRNA and protein-RNA interaction networks from large-scale CLIP-Seq data [J ] . Nucleic Acids Res , 2014 , 42 (Database issue): D92-D97.

TANG Z F , LI C W , KANG B X , et al. GEPIA: a web server for cancer and normal gene expression profiling and interactive analyses [J ] . Nucleic Acids Res , 2017 , 45 ( W1 ): W98-W102.

LI X , WANG C X , ZHANG H , et al. circFNDC3B accelerates vasculature formation and metastasis in oral squamous cell carcinoma [J ] . Cancer Res , 2023 , 83 ( 9 ): 1459 - 1475 .

GAO C F , GUO C C , LIU K , et al. LncRNA HOTAIR promotes proliferation and migration of OSCC cells via targeting miR-126 [J ] . Minerva Gastroenterol , 2022 , 68 ( 2 ): 245 - 247 .

SHI L J , YANG Y Q , LI M Y , et al. LncRNA IFITM4P promotes immune escape by up-regulating PD-L1 via dual mechanism in oral carcinogenesis [J ] . Mol Ther , 2022 , 30 ( 4 ): 1564 - 1577 . DOI: 10.1016/j.ymthe.2022.01.003 http://doi.org/10.1016/j.ymthe.2022.01.003

YANG Y X , CHEN D , LIU H , et al. Increased expression of lncRNA CASC9 promotes tumor progression by suppressing autophagy-mediated cell apoptosis via the AKT/mTOR pathway in oral squamous cell carcinoma [J ] . Cell Death Dis , 2019 , 10 ( 2 ): 41.

TAO D T , ZHANG Z X , LIU X , et al. LncRNA HOTAIR promotes the invasion and metastasis of oral squamous cell carcinoma through metastasis-associated gene 2 [J ] . Mol Carcinog , 2020 , 59 ( 4 ): 353 - 364 .

JIANG Y Y , WU K , CAO W , et al. Long noncoding RNA KTN1-AS1 promotes head and neck squamous cell carcinoma cell epithelial-mesenchymal transition by targeting miR-153-3p [J ] . Epigenomics , 2020 , 12 ( 6 ): 487 - 505 .

CHEN L L . Linking long noncoding RNA localization and function [J ] . Trends Biochem Sci , 2016 , 41 ( 9 ): 761 - 772 .

PASTUSHENKO I , BLANPAIN C . EMT transition states during tumor progression and metastasis [J ] . Trends Cell Biol , 2019 , 29 ( 3 ): 212 - 226 . DOI: S0962-8924(18)30201-0 http://doi.org/S0962-8924(18)30201-0

贾利晴 , 葛小路 , 姜琳 , 等 . LncRNA PKD2-2-3对肺腺癌细胞增殖、克隆形成、迁移及侵袭能力的影响 [J ] . 中国癌症杂志 , 2023 , 33 ( 8 ): 717 - 725 . DOI: 10.19401/j.cnki.1007-3639.2023.08.001 http://doi.org/10.19401/j.cnki.1007-3639.2023.08.001

JIA L Q , GE X L , JIANG L , et al. Effects of lncRNA PKD2-2-3 on cell proliferation, clone formation, migration, and invasion of lung adenocarcinoma [J ] . China Oncology , 2023 , 33 ( 8 ): 717 - 725 . DOI: 10.19401/j.cnki.1007-3639.2023.08.001 http://doi.org/10.19401/j.cnki.1007-3639.2023.08.001

THIERY J P , ACLOQUE H , HUANG R Y J , et al. Epithelial-mesenchymal transitions in development and disease [J ] . Cell , 2009 , 139 ( 5 ): 871 - 890 . DOI: 10.1016/j.cell.2009.11.007 http://doi.org/10.1016/j.cell.2009.11.007

LING Z H , CHENG B , TAO X A . Epithelial-to-mesenchymal transition in oral squamous cell carcinoma: challenges and opportunities [J ] . Int J Cancer , 2021 , 148 ( 7 ): 1548 - 1561 . DOI: 10.1002/ijc.33352 http://doi.org/10.1002/ijc.33352

JIANG Y Y , CAO W , WU K , et al. LncRNA LINC00460 promotes EMT in head and neck squamous cell carcinoma by facilitating peroxiredoxin-1 into the nucleus [J ] . J Exp Clin Cancer Res , 2019 , 38 ( 1 ): 365.

YOKOYAMA K , KAMATA N , HAYASHI E , et al. Reverse correlation of E-cadherin and snail expression in oral squamous cell carcinoma cells in vitro [J ] . Oral Oncol , 2001 , 37 ( 1 ): 65 - 71 .

HOXHAJ G , MANNING B D . The PI3K-AKT network at the interface of oncogenic signalling and cancer metabolism [J ] . Nat Rev Cancer , 2020 , 20 ( 2 ): 74 - 88 . DOI: 10.1038/s41568-019-0216-7 http://doi.org/10.1038/s41568-019-0216-7

XU W T , YANG Z , LU N H . A new role for the PI3K/Akt signaling pathway in the epithelial-mesenchymal transition [J ] . Cell Adh Migr , 2015 , 9 ( 4 ): 317 - 324 .

YE B , JIANG L L , XU H T , et al. Expression of PI3K/AKT pathway in gastric cancer and its blockade suppresses tumor growth and metastasis [J ] . Int J Immunopathol Pharmacol , 2012 , 25 ( 3 ): 627 - 636 .

FAN Q C , TIAN H , WANG Y , et al. Integrin-α5 promoted the progression of oral squamous cell carcinoma and modulated PI3K/AKT signaling pathway [J ] . Arch Oral Biol , 2019 , 101 : 85 - 91 .

CHAI S L , WEN Z H , ZHANG R X , et al. CCL25/CCR9 interaction promotes the malignant behavior of salivary adenoid cystic carcinoma via the PI3K/AKT signaling pathway [J ] . PeerJ , 2022 , 10 : e13844 .

LI Q , LI B , LU C L , et al. LncRNA LINC01857 promotes cell growth and diminishes apoptosis via PI3K/mTOR pathway and EMT process by regulating miR-141-3p/MAP4K4 axis in diffuse large B-cell lymphoma [J ] . Cancer Gene Ther , 2021 , 28 ( 9 ): 1046 - 1057 . DOI: 10.1038/s41417-020-00267-4 http://doi.org/10.1038/s41417-020-00267-4

Views

1659

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of SEC14L1P1 on proliferation and migration of oral squamous cell carcinoma cells

Study on the mechanism of DDX6 promoting proliferation and migration of nasopharyngeal carcinoma cells by regulating stability of CKMT1A mRNA

A study on mechanism of GOLM1 regulating PI3K/AKT/mTOR signaling pathway to promote proliferation, invasion and migration of lung adenocarcinoma cells

Effect ofPTP1Bgene on the proliferation and migration of human gastric cancer cell lines

Inhibition ofYAPgene by siRNA suppresses the proliferation and migration of T24 bladder cancer cells

Related Author

Wentian ZHENG

Hui GONG

Xinyue ZHANG

Jiayi HAO

Yajie WANG

Yingying JIANG

Jiayan XIONG

Wei LEI

Related Institution

Medical School of Nantong University

Department of Otolaryngology Head and Neck Surgery, Second Affiliated Hospital of Soochow University

Research Institute of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Nantong University

Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Nantong University

Department of Pathology, Karamay Central Hospital, Karamay 834000, Xinjiang Uygur Autonomous Region

AI问答

⁰