Background and purpose: Recent researches indicate that high grade serous ovarian carcinoma is derived from fallopian tube epithelial malignancy. In this study

we used primary fallopian tube epithelium to establish the immortalized cell lines by silencing of tumor suppression genes and introduction of hTERT

in order to further build malignant cell lines and ovarian cancer animal models. Methods: Primary fallopian tube epithelia were isolated and transfected with a plasmid containing pRb and p53 shRNAs combined with hTERT to establish immortalized cell lines. The resulting cells were validated through examining cell population doublings

p53 and pRb expression

SA-β-gal activity; anchorage-independent growth and in vivo tumorgenesis. Results: We successfully established two immortalized fallopian tube epithelial cell lines: FTE248116/p53i+pRbi+hTERT

FTE312249/p53i+pRbi+hTERT through silencing of p53 and pRb and introduction of hTERT simultaneously. Conclusion: The establishment of immortalized cell lines can be accomplished by introduction of p53 and Rb shRNA and overexpression of hTERT

and these cells may be used to establish the transformed cell lines and ovarian cancer animal models.