The role of miR-301 in colon cancer cell proliferation and apoptosis

张忠来; 熊欢; 张兵; 朱培谦

doi:10.3969/j.issn.1007-3969.2013.06.006

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The role of miR-301 in colon cancer cell proliferation and apoptosis

更新时间：2025-12-31

- The role of miR-301 in colon cancer cell proliferation and apoptosis
- China Oncology Vol. 23, Issue 6, Pages: 432-438(2013)
- 作者机构：
  
  南昌大学第二附属医院胃肠外科,江西,南昌,330006
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1007-3969.2013.06.006
  CLC：
- Published Online：13 November 2014，
  
  Published：2013
- 稿件说明：
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张忠来,熊欢,张兵,朱培谦. miR-301在结肠癌细胞增殖和凋亡中的作用[J]. 中国癌症杂志, 2013, 23(6): 432-438.

张忠来, 熊欢, 张兵, et al. The role of miR-301 in colon cancer cell proliferation and apoptosis[J]. China Oncology, 2013, 23(6): 432-438.
张忠来,熊欢,张兵,朱培谦. miR-301在结肠癌细胞增殖和凋亡中的作用[J]. 中国癌症杂志, 2013, 23(6): 432-438. DOI： 10.3969/j.issn.1007-3969.2013.06.006.

张忠来, 熊欢, 张兵, et al. The role of miR-301 in colon cancer cell proliferation and apoptosis[J]. China Oncology, 2013, 23(6): 432-438. DOI： 10.3969/j.issn.1007-3969.2013.06.006.

摘要

背景与目的：miR-301在多种恶性肿瘤中表达上调，然而其在结肠癌中的表达及功能尚不清楚。本研究旨在检测微小RNA miR-301在结肠癌组织中的表达，并在体内外研究miR-301反义寡核苷酸技术(ASO)对结肠癌细胞增殖和凋亡的影响。方法：运用荧光定量PCR定量分析120例结肠癌患者癌组织及对应癌旁组织中miR-301的表达；通过miR-301ASO降低结肠癌SW620细胞中miR-301的表达，采用MTT、克隆形成实验、流式细胞技术及体内实验观察miR-301ASO对SW620细胞产生的生物学效应。结果：在120例结肠癌患者中，63.33%(76/120)的结肠癌组织miR-301表达明显高于对应癌旁组织(P=0.00)；与对照组miR-301的表达量(0.50±0.07)相比，miR-301ASO组可以显著降低miR-301的表达(0.09±0.01，P=0.00)；MTT实验结果显示，转染miR-301 ASO组24、48、96 h SW620细胞的存活数量均明显低于对照组(P0.05)；克隆形成实验结果显示，miR-301ASO组克隆形成率(5.33%±0.74%)较对照组(33.33%±8.38%)显著降低(P=0.00)；体内研究进一步证实miR-301ASO可以抑制肿瘤细胞的增殖，从而导致肿瘤生长较对照组慢(P=0.01)，肿瘤的体积较对照组明显减小(P=0.01)；流式细胞仪检测显示，转染miR-301ASO组SW620细胞凋亡指数(15.68±1.46)较随机染转染ASO组(3.36±0.88)明显增高(P=0.02)；另外，降低miR-301的表达发现Bcl-2的mRNA和Bcl-2蛋白均明显下降(P=0.00，P=0.00)。结论：miR-301在结肠癌组织中表达上调，降低miR-301的表达可有效抑制结肠癌细胞生长、促进细胞凋亡。miR-301有可能成为结肠癌基因表达调控的新靶点。

Abstract

Background and purpose: The miR-224 in a variety of malignant tumors is overexpression

however

its expression and function in colon cancer are not clear. The aim of this study was to investigate the expression of miR-301 in colon cancer tissues and demonstrate the regulative effects of miR-301 ASO on the proliferation and apoptosis of colon cancer cell in vitro and in vitro. Methods: The expression of miR-301 in 120 colon cancer tissues and their adjacent tissues was detected by real-time quantitative PCR method. After transfection with miR-301ASO

the biological effects of miR-301 in SW620 cells were measured by MTT assay

the colony formation experiment

flow cytometry and the in vivo experiment. Results: The expression level of miR-301 was found to be overexpressed in 63.33% (76/120) of the colon cancer cases (P0.05). miR-301 expression in SW620 cells (transfection with miR-301 ASO

0.09±0.01) was significantly less than control group (0.50±0.07

P=0.00). MTT assay results showed that SW620 cells survived rate at 24

48 and 96 h decreased greatly after transfection with miR-301ASO (P=0.00). Clone formation assay revealed that miR-301 ASO group colony formation rate (5.33%±0.74%) was significantly lower than the control group (33.33%±8.38%

P=0.00). In vivo study further confirmed that miR-301ASO could inhibit the proliferation of SW620 cells (P0.05)

and miR-301ASO group grew substantially slow compared with the negative control group (P=0.00). Flow cytometry indicated that the apoptotic index in miR-301 ASO group (15.68±1.46) was significantly higher than the control group (3.36±0.88

P=0.02). In addition

the Bcl2 mRNA and protein were significantly decreased after reduce the expression of miR-301 (P=0.00

P=0.00). Conclusion: MiR-301 was overexpressed in human colon cancer. Reduce the expression of miR-301 can effectively inhibit the growth of colon cancer cells and promote apoptosis. MiR-301 may become a new target for the regulation of gene expression in colon cancer.

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