Down-regulation of microRNA-187* expression in colorectal cancer and its roles in promoting cell apoptosis

刘博; 田延锋; 赵增仁; 樊智彬; 张丽静; 贺新奇; 高利飞

doi:10.3969/j.issn.1007-3969.2013.09.002

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Down-regulation of microRNA-187* expression in colorectal cancer and its roles in promoting cell apoptosis

更新时间：2025-12-31

- Down-regulation of microRNA-187* expression in colorectal cancer and its roles in promoting cell apoptosis
- China Oncology Vol. 23, Issue 9, Pages: 703-708(2013)
- 作者机构：
  
  河北医科大学第一医院普通外科,河北,石家庄,050031
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1007-3969.2013.09.002
  CLC：
- Published Online：20 February 2014，
  
  Published：2013
- 稿件说明：
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刘博,田延锋,赵增仁,樊智彬,张丽静,贺新奇,高利飞. 微小RNA-187*在结直肠癌组织中表达及其对结肠癌细胞凋亡的影响[J]. 中国癌症杂志, 2013, 23(9): 703-708.

刘博, 田延锋, 赵增仁, et al. Down-regulation of microRNA-187* expression in colorectal cancer and its roles in promoting cell apoptosis[J]. China Oncology, 2013, 23(9): 703-708.
刘博,田延锋,赵增仁,樊智彬,张丽静,贺新奇,高利飞. 微小RNA-187*在结直肠癌组织中表达及其对结肠癌细胞凋亡的影响[J]. 中国癌症杂志, 2013, 23(9): 703-708. DOI： 10.3969/j.issn.1007-3969.2013.09.002.

刘博, 田延锋, 赵增仁, et al. Down-regulation of microRNA-187* expression in colorectal cancer and its roles in promoting cell apoptosis[J]. China Oncology, 2013, 23(9): 703-708. DOI： 10.3969/j.issn.1007-3969.2013.09.002.

摘要

背景与目的：微小RNA(microRNA

miRNA)在细胞分化、细胞周期及凋亡过程中发挥重要作用。miRNA通过扩增、缺失、突变和沉默等机制影响癌症的发生、发展。本研究探讨miR-187*在结直肠癌组织中的表达和临床意义，以及上调miR-187*表达量对结肠癌细胞凋亡的影响。方法：采用实时荧光定量反转录聚合酶链反应(real-time quantitative reverse transcription-PCR，real-time PCR)的方法在40例结直肠癌患者组织标本中检测miR-187*的表达水平，结合病理资料分析其临床意义。HCT116细胞转染miR-187*mimics后，用Annexin-Ⅴ FITC/PI流式细胞术检测miR-187*对凋亡的影响。结果：miR-187*在结直肠癌组织中表达量为0.165(0.106，0.428)，明显低于正常黏膜组织表达量［0.334(0.211，0.712)］，差异有统计学意义(P0.05)，且在黏液癌及高龄患者中下调更明显(P0.05)。将miR-187*mimics转染至HCT116中可提高其表达量，通过流式技术检测发现，与对照组早期凋亡率[(23.010±1.279)%]相比较，实验组早期凋亡率［(26.748±1.098)%］升高，差异有统计学意义(P＜0.05)。结论：miR-187*在结直肠癌组织中低表达，并且与组织学类型及年龄有关；miR-187*表达上调可促进HCT116早期凋亡；miR-187*具有潜在抑癌作用。

Abstract

Background and purpose: MicroRNAs (miRNAs) play an important role in tumor biological behavior. miRNAs are down-regulated or up-regulated in various cancer types

triggering abnormal cell differentiation

proliferation and apoptosis. This study was designed to investigate the expression and clinical significance of miR-187* in colorectal cancer (CRC)

and further to investigate its roles in promoting cell apoptosis. Methods: The expressions of miR-187* in 40 CRC cases were examined by real-time quantitative reverse transcription-PCR (qRT-PCR). The relationship between miR-187* expression and clinical features of CRC was analyzed. HCT116 cells were transfected with a miR-187* mimic and the apoptosis of the transfected cells were examined by flow cytometry (FCM). Results: The expression of miR-187* was down-regulated in CRC tissues 0.165 (0.106

0.428) compared with those in normal tissues 0.334 (0.211

0.712) (P0.05)

especially in mucinous carcinoma and older age CRC (P0.05). Transfection of HCT116 cells with a miR-187* mimic up-regulated the expression of miR-187* and increased cell early apoptosis (P0.05). Conclusion: The expression level of miR-187* was lower in CRC. miR-187* expression correlates with histological type and age. Transfection of HCT116 cells with a miR-187* mimic accelerates apoptosis of tumor cells

suggesting that miR-187* is a potent tumor suppressor.

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