The effects of plumbagin on proliferation and metastasis in human liver cancer SK-hep-1 cells

曹晓淬; 王卉; 张红梅; 刘怡雯; 卢忠心

doi:10.3969/j.issn.1007-3969.2013.09.05

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The effects of plumbagin on proliferation and metastasis in human liver cancer SK-hep-1 cells

更新时间：2025-12-31

- The effects of plumbagin on proliferation and metastasis in human liver cancer SK-hep-1 cells
- China Oncology Vol. 23, Issue 9, Pages: 721-727(2013)
- 作者机构：
  
  华中科技大学同济医学院附属武汉市中心医院检验科,湖北,武汉,430014
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1007-3969.2013.09.05
  CLC：
- Published Online：20 February 2014，
  
  Published：2013
- 稿件说明：
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曹晓淬,王卉,张红梅,刘怡雯,卢忠心. 白花丹醌对人肝癌SK-hep-1细胞增殖及侵袭的影响[J]. 中国癌症杂志, 2013, 23(9): 721-727.

曹晓淬, 王卉, 张红梅, et al. The effects of plumbagin on proliferation and metastasis in human liver cancer SK-hep-1 cells[J]. China Oncology, 2013, 23(9): 721-727.
曹晓淬,王卉,张红梅,刘怡雯,卢忠心. 白花丹醌对人肝癌SK-hep-1细胞增殖及侵袭的影响[J]. 中国癌症杂志, 2013, 23(9): 721-727. DOI： 10.3969/j.issn.1007-3969.2013.09.05.

曹晓淬, 王卉, 张红梅, et al. The effects of plumbagin on proliferation and metastasis in human liver cancer SK-hep-1 cells[J]. China Oncology, 2013, 23(9): 721-727. DOI： 10.3969/j.issn.1007-3969.2013.09.05.

摘要

背景与目的：白花丹醌是中药白花丹的主要活性成分，对多种肿瘤细胞具有杀伤作用。本研究旨在观察白花丹醌对人肝癌SK-hep-1细胞增殖及侵袭的影响，并初步探讨其作用机制。方法：在体外应用MTS法、软琼脂克隆形成实验、流式细胞术及Transwell小室观察白花丹醌对细胞增殖及侵袭的影响；并通过RTPCR检测白花丹醌对p21、MMP-2及MMP-9的mRNA表达影响。结果：白花丹醌能够明显抑制肝癌SK-hep-1细胞的增殖和克隆形成，且具有剂量依赖性，其半数抑制率为22.04 μ mol/L。细胞周期分析显示，白花丹醌处理后S期细胞数减少，G

期细胞增多；并且白花丹醌能够抑制SK-hep-1细胞的黏附和侵袭转移。RT-PCR结果显示白花丹醌能够促进p21表达，而抑制MMP-2及MMP-9的表达。结论：白花丹醌可能是通过上调p21及下调MMP-2/MMP-9的表达水平，抑制人肝癌SK-Hep-1细胞增殖和侵袭。

Abstract

Background and purpose: Plumbagin is the main active components of traditional Chinese medicine of plumbago zeylanica. The present studies show that plumbagin has a killing effect on tumor cells. This study aimed to investigate the function and primary mechanism of plumbagin on invasion and metastasis of human liver cancer SK-hep-1 cells. Methods: With the treatment of plumbagin in vitro

cell proliferation and adhesion of SK-hep-1 cells were detected by MTS staining

cell cycle of SK-hep-1 cells were detected by flow cytometry

the selfrenewal and propagation abilities of SK-Hep-1 cells were conducted by colony formation assay

invasion in cells were performed using transwell invasion assay

and the p21 and MMP-2/9 mRNA levels were detected by real-time RT-PCR. Results: Wit

h the treatment of plumbagin

SK-Hep-1 cells proliferation was decreased with plumbagin concentrationdependency and the IC

value of plumbagin in SK-Hep-1 cells was 22.04 mmol/L. The colony formation ability of SKHep-1 cells was decreased and the percentage of cells in G

phase was increased in a dose-dependent manner

as compared to control. The cell adhesion and invasion abilities were decreased. The real-time RT-PCR showed that p21 mRNA expression was increased and the MMP-2/9 mRNA was decreased. Conclusion: Plumbagin could suppress the proliferation and invasiveness of human liver cancer SK-hep-1 cells in vitro

and these effects may be by up-regulation of p21 and down-regulation of MMP-2 and MMP-9.

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