miR-21 downregulation attenuates cell proliferation, migration and invasion in nasopharyngeal carcinoma

周克兵; 谷刚; 曹昕

doi:10.3969/j.issn.1007-3969.2013.11.002

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miR-21 downregulation attenuates cell proliferation, migration and invasion in nasopharyngeal carcinoma

更新时间：2025-12-31

- miR-21 downregulation attenuates cell proliferation, migration and invasion in nasopharyngeal carcinoma
- China Oncology Vol. 23, Issue 11, Pages: 863-867(2013)
- 作者机构：
  
  南华大学附属南华医院急诊科,湖南,衡阳,421002
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1007-3969.2013.11.002
  CLC：
- Published Online：18 February 2014，
  
  Published：18 February 2013
- 稿件说明：
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周克兵,谷刚,曹昕. 下调miR-21表达抑制鼻咽癌CNE2细胞增殖和侵袭[J]. 中国癌症杂志, 2013, 23(11): 863-867.

周克兵, 谷刚, 曹昕. miR-21 downregulation attenuates cell proliferation, migration and invasion in nasopharyngeal carcinoma[J]. China Oncology, 2013, 23(11): 863-867.
周克兵,谷刚,曹昕. 下调miR-21表达抑制鼻咽癌CNE2细胞增殖和侵袭[J]. 中国癌症杂志, 2013, 23(11): 863-867. DOI： 10.3969/j.issn.1007-3969.2013.11.002.

周克兵, 谷刚, 曹昕. miR-21 downregulation attenuates cell proliferation, migration and invasion in nasopharyngeal carcinoma[J]. China Oncology, 2013, 23(11): 863-867. DOI： 10.3969/j.issn.1007-3969.2013.11.002.

摘要

背景与目的：miR-21在人类多种肿瘤中异常高表达。本研究探讨干扰miR-21表达对鼻咽癌CNE2细胞增殖、迁移和侵袭的影响。方法：使用脂质体将miR-21 inhibitor转染CNE2细胞，以无关序列(NC inhibitor)作为阴性对照。采用qRT-PCR技术验证miR-21 inhibitor转染的CNE2细胞中miR-21的表达水平；通过MTS法、细胞划痕、Transwell侵袭实验观察下调miR-21表达对CNE2细胞增殖、迁移和侵袭的影响。结果：转染miR-21 inhibitor的CNE2细胞中miR-21的表达明显下调，并且呈浓度依赖性。表明转染miR-21 inhibitor能有效抑制CNE2细胞中miR-21的表达。转染miR-21 inhibitor的CNE2细胞与对照细胞相比，增殖速度明显减慢，差异有统计学意义(P0.05)。细胞划痕实验显示，下调miR-21表达抑制CNE2细胞迁移(P0.05)。Transwell侵袭实验结果显示，下调miR-21表达抑制CNE2细胞侵袭(P0.05)。结论：miR-21能促进鼻咽癌细胞增殖、迁移和侵袭，其可能在鼻咽癌的发生、发展中发挥重要作用。

Abstract

Key words: Nasopharyngeal carcinoma

MiR-21

Cell proliferation

Cell invasion

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