The radiosensitizing effects of cryptotanshinone on H22 hepatoma-bearing mice

叶因涛; 王晨; 宋晓坤; 张洁; 高宁

doi:10.3969/j.issn.1007-3969.2014.01.005

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The radiosensitizing effects of cryptotanshinone on H22 hepatoma-bearing mice

更新时间：2025-12-31

- The radiosensitizing effects of cryptotanshinone on H22 hepatoma-bearing mice
- China Oncology Vol. 24, Issue 1, Pages: 29-34(2014)
- 作者机构：
  
  天津医科大学肿瘤医院药学部，国家肿瘤临床医学研究中心,天津市肿瘤防治重点实验室,天津,300060
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1007-3969.2014.01.005
  CLC：
- Published Online：14 February 2014，
  
  Published：14 February 2014
- 稿件说明：
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叶因涛,王晨,宋晓坤,张洁,高宁. 隐丹参酮对肝癌H22荷瘤小鼠放射增敏作用的影响[J]. 中国癌症杂志, 2014, 24(1): 29-34.

叶因涛, 王晨, 宋晓坤, et al. The radiosensitizing effects of cryptotanshinone on H22 hepatoma-bearing mice[J]. China Oncology, 2014, 24(1): 29-34.
叶因涛,王晨,宋晓坤,张洁,高宁. 隐丹参酮对肝癌H22荷瘤小鼠放射增敏作用的影响[J]. 中国癌症杂志, 2014, 24(1): 29-34. DOI： 10.3969/j.issn.1007-3969.2014.01.005.

叶因涛, 王晨, 宋晓坤, et al. The radiosensitizing effects of cryptotanshinone on H22 hepatoma-bearing mice[J]. China Oncology, 2014, 24(1): 29-34. DOI： 10.3969/j.issn.1007-3969.2014.01.005.

摘要

背景与目的：醌类化合物可使肿瘤细胞周期发生变化，影响放疗的敏感性。本研究旨在探讨隐丹参酮(cryptotanshinone)对荷H22肝癌小鼠的放射增敏作用及对细胞周期和凋亡的影响。方法：建立小鼠H22肝癌模型，分为空白对照组、单照射(irradiation，IR)组、高浓度隐丹参酮组及低、中、高浓度隐丹参酮联合IR组。经照射后，观察荷瘤鼠肿瘤生长状况，记录肿瘤照射后生长时间，计算肿瘤生长延缓时间和增敏系数。照后22 d处死小鼠，剥瘤称重，计算抑瘤率。流式细胞仪检测细胞周期和凋亡的变化。结果：不同浓度的隐丹参酮联合放疗均能抑制肿瘤生长，并具有较好的放射增敏作用，其增敏比分别为1.22、1.43和2.19。隐丹参酮可使肝癌H22细胞周期的构成发生明显的变化，使S期的细胞比例降低，出现明显的G

/M期阻滞，并诱导细胞凋亡。结论：隐丹参酮对肝癌H22荷瘤小鼠具有较高的抑瘤作用和放射增敏作用。其增敏效应使细胞生长停滞在G

/M期并诱导细胞凋亡，使S期细胞比例降低，从而加强射线对肿瘤细胞的杀伤能力。

Abstract

Background and purpose: Quinonoids can change the cell cycle distribution of tumor cells

and effect the radiosensitizing. This study aimed to investigate the radiosensitization effects

cell cycle and apoptosis of cryptotanshinone on H22 hepatoma-bearing mice. Methods: The mouse hepatoma H22 model was established

then divided into blank control group

irradiation alone group

high dose of cryptotanshinone group

cryptotanshinone (low

medium and high) + IR groups. After irradiated

observed the growth of tumor’s conditions

record epigenetic tumor irradiation time

calculated the delay time of tumor growth and enhancement factor (EF). After 22 days

mice were killed

stripped tumor

and calculated the inhibition rate. The cell cycle distribution and

apoptosis were measured by flow cytometry. Results: Cryptotanshinone (low

medium and high) groups inhibited the tumor growth better than the blank group

and had the significant radiosensitizing effect. The enhancement factor was 1.22

1.43

2.19

respectively. Cells were treated with cryptotanshinone which had significant effects on cell cycle

and induced apoptosis

which indicated significant G

/M phase arrest and a decrease in S phase. Conclusion: Cryptotanshinone inhibited the tumor growth and had the radiosensitizing effects on H22 hepatoma-bearing mice. One of the mechanism may be that it might make significant G

/M phase arrest and S phase decreased

and induced apoptosis. So cells were more sensitive to radiation.

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