Effect of<em>PTTG1</em>in the invasion of glioma cells

陈为一; 李小龙; 齐岳亮; 李洪利; 尹崇高; 刘晓丽; 张宝刚; 郭文君

doi:10.3969/j.issn.1007-3969.2014.05.002

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Effect ofPTTG1in the invasion of glioma cells

更新时间：2025-12-31

- Effect ofPTTG1in the invasion of glioma cells
- China Oncology Vol. 24, Issue 5, Pages: 329-332(2014)
- 作者机构：
  
  1. 潍坊医学院病理学教研室,山东,潍坊,261053
  2. 潍坊医学院医学研究实验中心,山东,潍坊,261053
  3. 潍坊医学院护理学院,山东,潍坊,261053
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1007-3969.2014.05.002
  CLC：
- Published Online：26 May 2014，
  
  Published：26 May 2014
- 稿件说明：
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陈为一,李小龙,齐岳亮,李洪利,尹崇高,刘晓丽,张宝刚,郭文君.

陈为一, 李小龙, 齐岳亮, et al. Effect ofPTTG1in the invasion of glioma cells[J]. China Oncology, 2014, 24(5): 329-332.
陈为一,李小龙,齐岳亮,李洪利,尹崇高,刘晓丽,张宝刚,郭文君. DOI： 10.3969/j.issn.1007-3969.2014.05.002.

陈为一, 李小龙, 齐岳亮, et al. Effect ofPTTG1in the invasion of glioma cells[J]. China Oncology, 2014, 24(5): 329-332. DOI： 10.3969/j.issn.1007-3969.2014.05.002.

摘要

背景与目的：研究证实垂体肿瘤转化基因1(pituitary tumor transforming gene 1，PTTG1)的表达情况与各类肿瘤细胞的恶性程度有关，但是其在胶质瘤中的作用尚不清楚。本研究旨在探讨PTTG1在恶性胶质瘤侵袭中的作用及其临床意义。方法：应用蛋白质印迹法(Western blot)检测PTTG1蛋白在不同胶质瘤细胞系中的表达；采用小RNA质粒干扰技术抑制U87细胞中PTTG1蛋白的表达，应用Western blot技术检测转染的效率；通过体外侵袭实验检测转染后细胞侵袭力的变化；采用Western blot技术检测经过表皮细胞生长因子(epithelial growth factor，EGF)刺激后敲除PTTG1的细胞组(siPTTG1/U87)与转染空载的细胞组(Scr/U87)中Akt、ARK5磷酸化的情况。结果：PTTG1蛋白在各恶性胶质瘤细胞系中均高表达；敲除PTTG1后U87细胞的侵袭力明显下降；对Scr/U87细胞进行EGF刺激5 min后，Akt、ARK5的磷酸化情况显著增强，而无论有无EGF的刺激，siPTTG1/U87中Akt、ARK5的磷酸化情况都没有明显改变。结论：在恶性胶质瘤细胞中，PTTG1蛋白呈高表达状态并且与侵袭性显著相关，其对侵袭性的调控可能是通过Akt-ARK5通路实现的。

Abstract

Background and purpose: Numerous researches indicated that the expression of pituitary tumor transforming gene1 (PTTG1) was correlated with the severity of glioma tumors. However the specific mechanism of PTTG1 is not clear in glioma. In this study

we explored the role and significance of PTTG1 in the invasion of glioma cells. Methods: Western blot was used to detect the expression of PTTG1 protein in various glioma cell lines. siRNA plasmid was used to transfect U87 cells. Western blot was used to analyze the expression of PTTG1 protein in transfected U87 cells. Matrigel invasion assay was used to detect the invasive ability in the cells being transfected in vitro. Western blot was used to analyze epithelial growth factor (EGF) induced protein phosphorylation of ARK5 and Akt in the cells being transfected PTTG1 plasmid (siPTTG1/U87) and scrambled siRNA (Scr/U87). Results: The expression of PTTG1 protein was higher in all glioma cell lines. After transfection

the invasion of siPTTG1/U87 was obviously decreased after 5 min with EGF stimulation than the Scr/U87

the phosphorylation of ARK5 and Akt was significantly enhanced. However

whether or not the existence of EGF

the phosphorylation of ARK5 and Akt had no differences in siPTTG1/U87. Conclusion: In glioma cells

PTTG1 protein is high expressed and maybe have an important function in glioma cells invasion through Akt-ARK5 signaling pathway.

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Effect ofPTTG1in the invasion of glioma cells

DOI：10.3969/j.issn.1007-3969.2014.05.002

摘要

Abstract

关键词

Keywords

references