Detection of<em>GATA5</em>gene methylation in plasma and stool of colorectal cancer and the clinical diagnosis

张学松; 张谢; 黄诗良; 陆宏娜; 王丹萍; 黄志刚

doi:10.3969/j.issn.1007-3969.2014.07.004

您当前的位置：

首页 >

文章列表页 >

Detection ofGATA5gene methylation in plasma and stool of colorectal cancer and the clinical diagnosis

更新时间：2025-12-31

- Detection ofGATA5gene methylation in plasma and stool of colorectal cancer and the clinical diagnosis
- China Oncology Vol. 24, Issue 7, Pages: 501-506(2014)
- 作者机构：
  
  1. 浙江省宁波市医疗中心李惠利医院消化内科,浙江,宁波,315040
  2. 宁波大学医学院,浙江,宁波,315211
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1007-3969.2014.07.004
  CLC：
- Published Online：29 July 2014，
  
  Published：29 July 2014
- 稿件说明：
移动端阅览
张学松,张谢,黄诗良,陆宏娜,王丹萍,黄志刚.

张学松, 张谢, 黄诗良, et al. Detection ofGATA5gene methylation in plasma and stool of colorectal cancer and the clinical diagnosis[J]. China Oncology, 2014, 24(7): 501-506.
张学松,张谢,黄诗良,陆宏娜,王丹萍,黄志刚. DOI： 10.3969/j.issn.1007-3969.2014.07.004.

张学松, 张谢, 黄诗良, et al. Detection ofGATA5gene methylation in plasma and stool of colorectal cancer and the clinical diagnosis[J]. China Oncology, 2014, 24(7): 501-506. DOI： 10.3969/j.issn.1007-3969.2014.07.004.

摘要

背景与目的：结直肠癌(colorectal cancer，CRC)是全球发病率第三，死亡率第四的恶性肿瘤。遗传学及表观遗传学的改变引发抑癌基因甲基化表达沉默是CRC发生的重要原因，本研究旨在探讨血浆、粪便GATA5启动子甲基化检测在CRC临床诊断中的应用价值。方法：收集34例健康体检者和43例CRC患者的血浆和配对的粪便标本。采用甲基化特异性PCR法(methylation specific PCR method，MSP)检测其血浆、粪便中GATA5甲基化水平，并分析其与临床病理特征相关性。结果：MSP结果显示CRC患者血浆、粪便中GATA5启动子甲基化率(60.74%、76.60%)高于健康者发生率(26.47%、32.35%)，差异均有统计学意义(P值分别为0.006 7，0.000 2)。CRC患者血浆甲基化发生率与临床分期(P=0.000 5)、淋巴结转移(P=0.020)密切相关，而粪便GATA5甲基化水平与临床病理特征无统计学意义。结论：检测粪便GATA5甲基化水平并辅以血浆GATA5甲基化水平可作为一种简单、非侵入、敏感及特异的方法应用于CRC患者的临床诊断。

Abstract

Background and purpose: Colorectal cancer (CRC) is a malignancy which is the third incidence and the fourth mortality in the worldwide. The main reason for the development of CRC is that the changes of genetic and epigenetic causes the tumor suppressor gene methylation silencing. This study aimed to investigate the plasma and stool GATA5 gene promoter methylation was detected in clinical diagnosis of CRC. Methods: To collect the paired plasma and stool specimens of 34 cases of healthy and 43 cases of patients with CRC. Methylation specific PCR (MSP) was respectively detected the GATA5 gene methylation levels of GATA5 gene in plasma and stool. And then separately analyzed their correlations with clinical and pathological parameters in gastric carcinoma. Results: The result of MSP showed that GATA5 gene promoter methylation rates in plasma and stool of CRC patients were 60.74%

76.60%

respectively

were higher than those of healthy persons (6.47%

32.35%). And the differences were statistically significant (P=0.006 7

P=0.000 2

respectively). GATA5 gene methylation rates in plasma of CRC patients were closely related to clinical stage (P=0.000 5) and lymph node metastasis(P=0.020)

while GATA5 gene methylation rates in stool of CRC patients had no significant with clinical and pathological parameters. Conclusion: Detection of faecal GATA5 gene methylation level and supplemented plasma GATA5 gene methylation level can become a simple

non-invasive

sensitive and specific method for clinical diagnosis of CRC.

关键词

Keywords

references

Views

2212

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Detection of HIC1 promoter methylation in prostate cancer using MSP and BSP methods

The relationship between methylation of ERCC1 gene in peripheral blood and in gastric cancer tissues

Targeted therapy and immunotherapy combined with chemotherapy bring effective response to AFP positive colon cancer patients: a case report and literature review

Mechanisms underlying tepoxalin-mediated regulation of cell growth and chemoresistance via ABCB1 in colorectal cancer

Research progress and prospects of exercise intervention in comprehensive management of colorectal cancer

Related Author

单孟林

丁　滔

郑江花

王红兵

陈卫昌

LIU Miao

LI Jinze

WU Chensi

Related Institution

复旦大学附属公共卫生临床中心医学检验科

上海交通大学附属第六人民医院南院泌尿外科

苏州大学附属第一医院消化内科

徐州医学院附属医院肿瘤内科

Department of Gastroenterology, the Fourth Hospital of Hebei Medical University

AI问答

⁰

Detection ofGATA5gene methylation in plasma and stool of colorectal cancer and the clinical diagnosis

DOI：10.3969/j.issn.1007-3969.2014.07.004

摘要

Abstract

关键词

Keywords

references