The expression and clinical significance of microRNA-192 and ZEB2 in colorectal cancer

吴巍芸; 周宇

doi:10.3969/j.issn.1007-3969.2014.07.005

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The expression and clinical significance of microRNA-192 and ZEB2 in colorectal cancer

更新时间：2025-12-31

- The expression and clinical significance of microRNA-192 and ZEB2 in colorectal cancer
- China Oncology Vol. 24, Issue 7, Pages: 507-511(2014)
- 作者机构：
  
  广东医学院附属医院消化内科,广东,湛江,524001
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1007-3969.2014.07.005
  CLC：
- Published Online：29 July 2014，
  
  Published：29 July 2014
- 稿件说明：
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吴巍芸,周宇. miR-192与ZEB2在结直肠癌中的表达及其临床意义[J]. 中国癌症杂志, 2014, 24(7): 507-511.

吴巍芸, 周宇. The expression and clinical significance of microRNA-192 and ZEB2 in colorectal cancer[J]. China Oncology, 2014, 24(7): 507-511.
吴巍芸,周宇. miR-192与ZEB2在结直肠癌中的表达及其临床意义[J]. 中国癌症杂志, 2014, 24(7): 507-511. DOI： 10.3969/j.issn.1007-3969.2014.07.005.

吴巍芸, 周宇. The expression and clinical significance of microRNA-192 and ZEB2 in colorectal cancer[J]. China Oncology, 2014, 24(7): 507-511. DOI： 10.3969/j.issn.1007-3969.2014.07.005.

摘要

背景与目的：微小RNA(microRNA，miRNA)是一类具有重要调控作用的非编码小分子RNA，在多种疾病的发生、发展中起重要作用。本研究通过检测结直肠癌(colorectal cancer，CRC)组织中miR-192及E盒结合锌指蛋白2(zinc finger E-box binding homeobox 2，ZEB2) mRNA、蛋白表达，分析miR-192的异常表达与CRC的临床病理特征的关系及与ZEB2的相关性。方法：选择CRC组织和癌旁组织(距肿瘤边缘≥5 cm)各30例，结直肠腺瘤组织25例，正常结直肠组织15例。实时定量PCR(quantitative real-time polymerase chainreation，qRT-PCR)法检测上述各组中miR-192及ZEB2 mRNA表达；蛋白质印迹法(Western blot)检测ZEB2蛋白表达；对CRC组织中miR-192与临床病理特征关系及前者与ZEB2的相关性进行分析。结果：CRC组miR-192表达明显下调，ZEB2 mRNA和蛋白表达明显上调，分别与癌旁组、结直肠腺瘤组及正常组相比，差异均有统计学意义(P0.05)；miR-192的表达下调与CRC的淋巴结转移(P=0.021)和远处转移(P=0.023)相关。在CRC组织中，miR-192与ZEB2蛋白的表达呈显著的负相关关系(r=-0.365，P0.05)。结论：miR-192在CRC组织中的表达下调与肿瘤的转移有关，且可能通过ZEB2参与CRC的发生、发展过程。

Abstract

Background and purpose: MicroRNA(miRNA) is a class of small non-coding RNA playing an important regulatory role in many diseases. In this study

we explore the levels of miR-192 and zinc finger E-box binding homeobox 2 (ZEB2) in CRC

the clinical significance of miR-192 and the correlation between the expression of miR-192 and ZEB2 protein. Methods: The expression levels of miR-192 and ZEB2 mRNA in 30 colorectal carcinoma samples

30 corresponding cancer-adjacent tissue samples (from the edge of tumor ≥5 cm)

25 colorectal adenoma samples

and 15 normal tissue samples were quantified using quantitative real-time polymerase chain reaction (qRT-PCR). ZEB2 protein expression was determined using Western blot. The relationship between the miR-192 and clinicopathological factors

miR-192 and ZEB2 protein expression was analyzed. Results: Significant upregulation of miR-192 expression and reduction of ZEB2 mRNA and protein expression were identified in CRC tissues

compared to cancer-adjacent tissues

colorectal adenoma samples

and normal tissues (P0.05). Low miR-192 levels were significantly associated with lymph node (P=0.021) and distant metastasis (P=0.023). An inverse relationship between miR-192 and ZEB2 protein expression was identified in CRC group (r=-0.365

P0.05). Conclusion: MiR-192 downexpression was correlated with CRC metastasis. MiR-192 may play a role in the development and progression of CRC through ZEB2.

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