The effect of miR-340 on proliferation and apoptosis of breast cancer cell MDA-MB231

欧阳倩雯; 曹亚丽

doi:10.3969/j.issn.1007-3969.2014.07.007

您当前的位置：

首页 >

文章列表页 >

The effect of miR-340 on proliferation and apoptosis of breast cancer cell MDA-MB231

更新时间：2025-12-31

- The effect of miR-340 on proliferation and apoptosis of breast cancer cell MDA-MB231
- China Oncology Vol. 24, Issue 7, Pages: 517-520(2014)
- 作者机构：
  
  江西省南昌市第三医院乳腺科,江西,南昌,330009
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1007-3969.2014.07.007
  CLC：
- Published Online：29 July 2014，
  
  Published：29 July 2014
- 稿件说明：
移动端阅览
欧阳倩雯,曹亚丽. miR-340对乳腺癌MDA-MB231细胞增殖和凋亡的影响[J]. 中国癌症杂志, 2014, 24(7): 517-520.

欧阳倩雯, 曹亚丽. The effect of miR-340 on proliferation and apoptosis of breast cancer cell MDA-MB231[J]. China Oncology, 2014, 24(7): 517-520.
欧阳倩雯,曹亚丽. miR-340对乳腺癌MDA-MB231细胞增殖和凋亡的影响[J]. 中国癌症杂志, 2014, 24(7): 517-520. DOI： 10.3969/j.issn.1007-3969.2014.07.007.

欧阳倩雯, 曹亚丽. The effect of miR-340 on proliferation and apoptosis of breast cancer cell MDA-MB231[J]. China Oncology, 2014, 24(7): 517-520. DOI： 10.3969/j.issn.1007-3969.2014.07.007.

摘要

背景与目的：既往研究表明，微小RNA-340(miR-340)能负性调控多种肿瘤的进展，但其在乳腺癌细胞增殖和凋亡中的研究较少，本研究旨在探讨miR-340对乳腺癌MDA-MB231细胞增殖和凋亡的作用。方法：利用脂质体LipofectamineTM2000将pre-miR-340或anti-miR-340瞬时转染至乳腺癌MDA-MB231细胞，通过RT-PCR检测miR-340 mRNA的水平，蛋白质印迹法(Western blot)检测cleaved-caspase-3蛋白的表达，MTT比色法检测细胞增殖的抑制情况，流式细胞仪检测细胞凋亡。结果：Pre-miR-340增加了MDA-MB231细胞中miR-340表达，同时增加cleaved-caspase-3蛋白表达、抑制MDA-MB231细胞增殖并促进其凋亡。而anti-miR-340抑制了MDAMB231细胞中miR-340表达，并且抑制cleaved-caspase-3蛋白表达，促进了MDA-MB231细胞增殖，抑制了MDAMB231细胞的凋亡。结论：miR-340转染后能上调MDA-MB231细胞中cleaved-caspase-3蛋白的表达从而抑制细胞增殖，促进其凋亡。

Abstract

Background and purpose: MicroRNA-340 (miR-340) has been demonstrated to play a role of negative regulation in many kinds of tumor

however

there are few reports about the relationship between miR-340 in proliferation and apoptosis of breast cancer cell. This study was aimed to explore the effect of miR-340 on proliferation and apoptosis of breast cancer cell MDA-MB231. Methods: The pre-miR-340 or anti-miR-340 were transiently transfected into breast cancer cell MDA-MB231 with LipofectamineTM2000. miR-340 level was detected by RT-PCR. The Western blot was performed to detect the protein level of cleaved-caspase-3. The inhibition rate of cell proliferation was evaluated by MTT assay. The cell apoptosis was studied by flow cytometry. Results: The pre-miR-340 facilitated the expression of miR-340 in MDA-MB231 cells. The pre-miR-340 enhanced the protein level of cleaved-caspase-3

inhibited the proliferation of MDA-MB231 cells and increased its apoptosis. On the contrary

the expression of miR-340 was inhibited by anti-miR-340 in MDA-MB231 cells. The protein level of cleaved-caspase-3 was reduced after the antimiR-340-transfected MDA-MB231 cells. Anti-miR-340 promoted the proliferation of MDA-MB231 cells. Decreased apoptosis of MDA-MB231 cells was observed by flow cytometry. Conclusion: The overexpression of miR-340 can effectively inhibit the proliferation and increase the apoptosis of MDA-MB231 cells

which may be explained by upregulating of protein cleaved-caspase-3 level.

关键词

Keywords

references

Views

1697

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Mechanistic study on PLK4 regulation of invasion, proliferation and apoptosis in oral squamous cell carcinoma

The influence ofPRUNE2 gene point mutation on proliferation, apoptosis, invasion and migration of prostate cancer DU145 cells

miR-122-5p inhibits the growth of esophageal cancer cells and transplanted tumors by targeting CREB1

Effect of glioma-associated oncogene homolog 2 silencing on proliferation and apoptosis of colon cancer cell line SW620

ARHGAP4 promotes growth of hepatocellular carcinoma cells by regulating HK2 expression

Related Author

Huan QIN

Jie WANG

Jie YANG

Yingjie HUA

Huijuan QU

Honghai JI

曹达龙

朱文恺

Related Institution

Department of Stomatology, Affiliated Hospital of Shandong Second Medical University

Shouguang Stomatological Hospital

School of Stomatology, Shandong Second Medical University

复旦大学附属肿瘤医院泌尿外科，复旦大学上海医学院肿瘤学系

福建医科大学附属泉州第一医院胸外科

AI问答

⁰