Methylation status and expression of RASSF10 gene in gastric cardia adenocarcinoma

崔建利; 郭炜; 郭艳丽; 沈素朋; 董稚明

doi:10.3969/j.issn.1007-3969.2014.08.002

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Methylation status and expression of RASSF10 gene in gastric cardia adenocarcinoma

更新时间：2025-12-31

- Methylation status and expression of RASSF10 gene in gastric cardia adenocarcinoma
- China Oncology Vol. 24, Issue 8, Pages: 568-574(2014)
- 作者机构：
  
  河北医科大学第四医院河北省肿瘤研究所病理研究室,河北,石家庄,050011
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1007-3969.2014.08.002
  CLC：
- Published Online：07 November 2014，
  
  Published：07 November 2014
- 稿件说明：
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崔建利,郭炜,郭艳丽,沈素朋,董稚明.

崔建利, 郭炜, 郭艳丽, et al. Methylation status and expression of RASSF10 gene in gastric cardia adenocarcinoma[J]. China Oncology, 2014, 24(8): 568-574.
崔建利,郭炜,郭艳丽,沈素朋,董稚明. DOI： 10.3969/j.issn.1007-3969.2014.08.002.

崔建利, 郭炜, 郭艳丽, et al. Methylation status and expression of RASSF10 gene in gastric cardia adenocarcinoma[J]. China Oncology, 2014, 24(8): 568-574. DOI： 10.3969/j.issn.1007-3969.2014.08.002.

摘要

背景与目的：Ras相关域家族10基因(Ras-association domain family 10，RASSF10)在多种肿瘤组织中具有肿瘤抑制功能，然而在贲门腺癌组织中的研究尚未见报道。检测贲门腺癌(gastric cardia adenocarcinoma，GCA)组织中RASSF10基因的甲基化状态及表达情况，进一步探讨RASSF10基因在GCA发生、发展中的作用。方法：分别应用甲基化特异性聚合酶链式反应(methylation specific polymerase chain reaction，MSP)、逆转录-聚合酶链式反应(reverse transcription-polymerase chain reaction，RT-PCR)及免疫组织化学方法检测81例GCA患者癌组织及癌旁正常组织中RASSF10基因的甲基化状态及mRNA和蛋白表达情况。结果：RASSF10基因在GCA组织中的启动子区甲基化率(64.2%，52/81)显著高于癌旁正常组织(21.0%，17/81，P0.05)，并与肿瘤组织的TNM分期、分化程度及淋巴结转移相关(P均0.05)。RASSF10基因在GCA组织中的mRNA表达量(0.57±0.05)显著低于癌旁正常组织(0.78±0.02，P0.05)，并与肿瘤组织的TNM分期及淋巴结转移相关(P均0.05)。RASSF10蛋白在GCA组织中的阳性表达率(31.1%，26/81)明显低于癌旁正常组织(71.6%，58/81，P0.05)，并与肿瘤组织的TNM分期、分化程度及淋巴结转移相关(P均0.05)。RASSF10基因在GCA组织中的甲基化率与其蛋白表达呈明显的负相关。结论：RASSF10基因启动子区的异常高甲基化导致的基因沉默可能是GCA发生的机制之一。

Abstract

Background and purpose: RASSF10 acts as a kind of tumor suppressor in various tumor tissues

but researches in cardiac adenocarcinoma has not been reported. This study aimed to detect the methylation status and expression of Ras-association domain family 10 (RASSF10) in gastric cardia adenocarcinoma (GCA)

and explore its role in occurrence and development of GCA. Methods: Methylation specific polymerase chain reaction (MSP)

reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry method were respectively used to detect methylation status

mRNA expression and protein expression of RASSF10 in 81 GCA tissues and corresponding normal tissues.Results: The promoter methylation frequency of RASSF10 in GCA tissues (64.20%

52/81) was significantly higher than that in corresponding normal tissues (20.99%

17/81

P0.05)

and was closely correlated with TNM stages

differential degree and lymph node metastasis (P0.05). RASSF10 mRNA expression in GCA tissues (0.57±0.05) was significantly lower than that in corresponding normal tissues (0.78±0.02

P0.05)

and was closely correlated with TNM stages and lymph node metastasis (P0.05). Protein expression of RASSF10 in GCA tissues (31.10%

26/81) was significantly lower than that in corresponding normal tissues (71.60%

58/81

P0.05)

and was closely correlated with TNM stages

differential degree and lymph node metastasis (P0.05). The promoter methylation frequency of RASSF10 in GCA tissues was inversely related to its protein expression.Conclusion: Inactivation of RASSF10 caused by aberrant methylation in the promoter region may be closely correlated with the GCA tumorgenesis.

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